Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

1984 ◽  
Vol 39 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Friednch Drawert ◽  
Ralf G. Berger ◽  
Rolf Godelmann ◽  
Susanne Collin ◽  
Wolfgang Barz
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
Straight Chain ◽  
Methylbutyric Acid ◽  
Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantita­tively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

Characterization of Volatile Constituents from Heterotrophic Cell Suspension Cultures of Ruta graveolens

1986 ◽  
Vol 41 (9-10) ◽  
pp. 809-812 ◽  
Author(s):  
Michael Jordan ◽  
Claus H. Rolfs ◽  
Wolfgang Barz ◽  
Ralf G. Berger ◽  
Hubert Kollmannsberger ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Chemical Composition ◽  
Cell Suspension ◽  
Mass Spectroscopy ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Secondary Alcohols ◽  
Ruta Graveolens

Heterotrophic cell suspension cultures of Ruta graveolens were established by reversion of photomixotrophic cultures without any change in the chemical composition of the growth medium. The heterotrophic cultures were qualitatively and quantitatively analyzed by gas- chromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, C6-C8 ketones, acetic acid n-butylester and a series of aliphatic C4-C9 primary and secondary alcohols were found as main constituents. Two isomeric sabinene hydrates were also isolated as new constituents of rue cells. The data are discussed in comparison to results obtained with photomixotrophic cell suspension cultures.

Isolation and characterization of an UDPG-dependent glucosyltransferase activity from Rauwolfia serpentina Benth. cell suspension cultures

1994 ◽  
Vol 72 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Ralf Lutterbach ◽  
Carl Michael Ruyter ◽  
Joachim Stöckigt
Keyword(s):  
Enzyme Activity ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Rauwolfia Serpentina ◽  
Isolation And Characterization ◽  
Membrane Bound ◽  
Plant Families

From cell suspension cultures of Rauwolfia serpentina Benth. a new enzyme activity was isolated and its properties determined. The enzyme is a soluble protein and catalyzes the transfer of a glucose moiety from UDPG to a wide variety of phenolic compounds with p-nitrophenol as one of the best substrates (Km = 1.21 mM, UDPG = 0.54 mM). In contrast to the membrane-bound UDPG: vomilenine-21-OH-β-D-glucosyltransferase from Rauwolfia serpentina cells, this enzyme is not able to glucosylate indole alkaloids. The enzyme activity has been detected in 14 callus cultures belonging to 10 different plant families.

Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures

2007 ◽  
Vol 51 (2) ◽  
pp. 293-302 ◽  
Author(s):  
Song-Jae Lee ◽  
Cheon-Ik Park ◽  
Mi-Young Park ◽  
Hahn-Sun Jung ◽  
Wuk-Sang Ryu ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals Demonstration of lactase activity in culture medium of melon cells

2011 ◽  
Vol 31 (No. 4) ◽  
pp. 132-135
Author(s):  
J. Stano ◽  
K. Mičieta ◽  
E. Tokhtaeva ◽  
M. Valšíková ◽  
M. Koreňová ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Culture Medium ◽  
Agar Plate ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Lactase Activity ◽  
Seedling Roots ◽  
Intracellular Activities

Lactase activity was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple, rapid and reproducible procedure for identification of extracellular lactase is described using callus cultures of seedlings from the tested plant, hairy roots of 2.5 days old seedlings of watermelon germinating on agar plates as well as cell suspension cultures derived from callus cultures. For the determination of intracellular activities of lactase, 6-bromo-2-naphthyl-β-D-galactopyranoside and p-nitrophenyl-β-D-galactopyranoside were used as synthetic substrates. The extracellular lactase activity was determined by evaluating the day-zone in agar medium. The enzyme from watermelon callus cultures and seedling roots, cultivated on agar plates supplemented with 6-bromo-2-naphthyl-2-bromo-β-D-galactopyranoside, hydrolyzed this substrate releasing 6-bromo-naphthyl. By simultaneous coupling with hexazonium p-rosaniline or Fast Blue BB the corresponding azo dye was formed. The parallel extracellular and intracellular activities were determined in cell suspension cultures derived from callus cultures. The results show a 43.8% intracellular and 54.2% extracellular distribution of lactase activity. The described agar plate method enables a rapid, simple and specific detection of plant processes of extracellular lactase.  

scholarly journals Further Characterization of Expression of Auxin-Induced Genes in Tobacco (Nicotiana tabacum) Cell-Suspension Cultures

1993 ◽  
Vol 102 (2) ◽  
pp. 513-520 ◽  
Author(s):  
KJM. Boot ◽  
B. J. van der Zaal ◽  
J. Velterop ◽  
A. Quint ◽  
A. M. Mennes ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Induced Genes
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