Collybial, a New Antibiotic Sesquiterpenoid from Collybia confluens (Basidiomycetes)

1995 ◽  
Vol 50 (3-4) ◽  
pp. 173-180 ◽  
Author(s):  
Brigitte Simon ◽  
Timm Anke ◽  
U. Andersb ◽  
M. Neuhausb ◽  
F. Hansske
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Pinus Koraiensis ◽  
Spectroscopic Methods ◽  
Baby Hamster Kidney ◽  
Gram Positive ◽  
Cytotoxic Effects ◽  
Bhk Cells ◽  
Gram Positive Bacteria ◽  
American Strain ◽  
Vesicular Stomatitis

A new antibiotic was isolated from fermentations of an american strain of Collybia confluens. Its structure was elucidated by spectroscopic methods as 2,10,10-trimethal-4-oxo-tricyclo[7.2.0.02.5]undec-6-en-carbaldehyde (with the relative stereo chemistry IS, 2R, 5R, 9R) (1). The inhibitor, which was named collybial, is structurally related to koraiol, a sesquiterpenoid isolated from Pinus koraiensis (Khan V. A. (1979), Khim. Prir. Soedin 5, 652-658). Collybial inhibited the growth of Gram-positive bacteria at concentrations starting from 21.5 μm . The propagation of vesicular stomatitis virus (VSV) in baby hamster kidney (BHK-21) cells was inhibited by 21.5 μm collybial. Cytotoxic effects on BHK cells were observed at 5 fold higher concentrations.

Intracellular formation of two soluble glycoproteins in BHK cells infected with vesicular stomatitis virus serotype New Jersey

Virology ◽  
1991 ◽  
Vol 180 (2) ◽  
pp. 678-686 ◽  
Author(s):  
Jürgen Gronberg ◽  
Antonie Kruppa ◽  
Peter Paschen ◽  
Joachim Kruppa
Keyword(s):  
New Jersey ◽  
Vesicular Stomatitis Virus ◽  
Bhk Cells ◽  
Virus Serotype ◽  
Vesicular Stomatitis

scholarly journals Identification of a Novel Tripartite Complex Involved in Replication of Vesicular Stomatitis Virus Genome RNA

2003 ◽  
Vol 77 (1) ◽  
pp. 732-738 ◽  
Author(s):  
Ashim K. Gupta ◽  
Daniel Shaji ◽  
Amiya K. Banerjee
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Reverse Genetics ◽  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Wild Type ◽  
Bhk Cells ◽  
P Protein ◽  
Vesicular Stomatitis

ABSTRACT Our laboratory's recent observations that transcriptionally inactive phosphoprotein (P) mutants can efficiently function in replicating vesicular stomatitis virus (VSV) defective interfering particle in a three-plasmid-based (L, P, and N) reverse genetics system in vivo (A. K. Pattnaik, L. Hwang, T. Li, N. Englund, M. Mathur, T. Das, and A. K. Banerjee, J. Virol. 71:8167-8175, 1997) led us to propose that a tripartite complex consisting of L-(N-P) protein may represent the putative replicase for synthesis of the full-length genome RNA. In this communication we demonstrate that such a complex is indeed detectable in VSV-infected BHK cells. Furthermore, coexpression of L, N, and P proteins in Sf21 insect cells by recombinant baculovirus containing the respective genes also resulted in the formation of a tripartite complex, as shown by immunoprecipitation with specific antibodies. A basic amino acid mutant of P protein, P260A, previously shown to be inactive in transcription but active in replication (T. Das, A. K. Pattnaik, A. M. Takacs, T. Li, L. N. Hwang, and A. K. Banerjee, Virology 238:103-114, 1997) was also capable of forming the mutant [L-(N-Pmut)] complex in both insect cells and BHK cells. Sf21 extract containing either the wild-type P protein or the mutant P protein along with the L and N proteins was capable of synthesizing 42S genome-sense RNA in an in vitro replication reconstitution reaction. Addition of N-Pmut or wild-type N-P complex further stimulated the synthesis of the genome-length RNA. These results indicate that the transcriptase and replicase complexes of VSV are possibly two distinct entities involved in carrying out capped mRNAs and uncapped genome and antigenome RNAs, respectively.

Coprinol, a New Antibiotic Cuparane from a Coprinus Species

2001 ◽  
Vol 56 (1-2) ◽  
pp. 31-34 ◽  
Author(s):  
Martin Johansson ◽  
Olov Sterner ◽  
Harald Labischinski ◽  
Timm Anke
Keyword(s):  
Biological Activities ◽  
Parent Compound ◽  
Multidrug Resistant ◽  
Spectroscopic Methods ◽  
Gram Positive ◽  
Gram Positive Bacteria

Abstract Coprinol, a new antibacterial cuparane, was isolated from fermentations of a Coprinus sp. Its biological activities were investigated and its structure was elucidated by spectroscopic methods. The new antibiotic exhibited activitiy against multidrug-resistant Gram -positive bacteria in vitro. Two derivatives were synthesized and their activities compared to the parent compound.

scholarly journals Rapid inhibition of pinocytosis in baby hamster kidney (BHK-21) cells following infection with vesicular stomatitis virus.

1983 ◽  
Vol 97 (5) ◽  
pp. 1444-1451 ◽  
Author(s):  
D K Wilcox ◽  
P A Whitaker-Dowling ◽  
J S Youngner ◽  
C C Widnell
Keyword(s):  
Horseradish Peroxidase ◽  
Vesicular Stomatitis Virus ◽  
Viral Gene Expression ◽  
Wild Type Virus ◽  
Viral Gene ◽  
Temperature Sensitive ◽  
Cell Fractionation ◽  
Baby Hamster Kidney ◽  
Transmembrane Glycoprotein ◽  
Vesicular Stomatitis

Infection of baby hamster kidney cells with vesicular stomatitis virus (VSV) caused a reduced rate of pinocytosis (as judged by the uptake of horseradish peroxidase) after 1 h, and maximum inhibition (60-80%) was observed at 4-6 h. This inhibition occurred 2-3 h before release of virus or changes in cell morphology. Analytical cell fractionation of homogenates of VSV-infected cells indicated that the horseradish peroxidase taken up by pinocytosis was transferred to lysosomes. The inhibition of pinocytosis required viral gene expression: little or no inhibition was detected in cells infected with UV-irradiated virus, wild-type virus in the presence of cycloheximide, or a temperature-sensitive mutant which failed to synthesize viral proteins. When cells were infected with temperature-sensitive viruses with mutations in the five VSV genes, an inhibition of pinocytosis was observed only when the viral transmembrane glycoprotein was present on the surface of the cells.

scholarly journals Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3339
Author(s):  
Jeferson Rodrigo Souza Pina ◽  
João Victor Silva-Silva ◽  
Josiwander Miranda Carvalho ◽  
Heriberto Rodrigues Bitencourt ◽  
Luciano Almeida Watanabe ◽  
...  
Keyword(s):  
Natural Compound ◽  
Peritoneal Macrophages ◽  
Gram Positive ◽  
Cytotoxic Effects ◽  
Gram Positive Bacteria ◽  
Ic50 Value ◽  
Exserohilum Rostratum ◽  
Hepatocarcinoma Cells ◽  
Microbroth Dilution ◽  
Intracellular Amastigote

The natural compound ravenelin was isolated from the biomass extracts of Exserohilum rostratum fungus, and its antimicrobial, antiplasmodial, and trypanocidal activities were evaluated. Ravenelin was isolated by column chromatography and HPLC and identified by NMR and MS. The susceptibility of Gram-positive and Gram-negative bacteria strains to ravenelin was determined by microbroth dilution assay. Cytotoxicity was evaluated in hepatocarcinoma cells (HepG2) and BALB/c peritoneal macrophages by using MTT. SYBR Green I-based assay was used in the asexual stages of Plasmodium falciparum. Trypanocidal activity was tested against the epimastigote and intracellular amastigote forms of Trypanosoma cruzi. Ravenelin was active against Gram-positive bacteria strains, with emphasis on Bacillus subtilis (MIC value of 7.5 µM). Ravenelin’s antiparasitic activities were assessed against both the epimastigote (IC50 value of 5 ± 1 µM) and the intracellular amastigote forms of T. cruzi (IC50 value of 9 ± 2 µM), as well as against P. falciparum (IC50 value of 3.4 ± 0.4 µM). Ravenelin showed low cytotoxic effects on both HepG2 (CC50 > 50 µM) and peritoneal macrophage (CC50 = 185 ± 1 µM) cells with attractive selectivity for the parasites (SI values > 15). These findings indicate that ravenelin is a natural compound with both antibacterial and antiparasitic activities, and considerable selectivity indexes. Therefore, ravenelin is an attractive candidate for hit-to-lead development.

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