Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

2010 ◽  
Vol 65 (7-8) ◽  
pp. 501-507 ◽  
Author(s):  
Wei Wei ◽  
Xiao Dai ◽  
Yong Wang ◽  
Chun-Bao Gou ◽  
Fang Chen
Keyword(s):  
Drought Stress ◽  
Ricinus Communis ◽  
Cloning And Expression ◽  
Transcript Levels ◽  
Reading Frame ◽  
Inositol 1 ◽  
Ricinus Communis L ◽  
Relative Water ◽  
Water Contents ◽  
Phosphate Synthase

A novel 1 L-myo-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) gene, designate rcMIPS, was cloned from Ricinus communis. It contained an open reading frame (ORF) of 1669 bp coding for a peptide of 510 amino acids with a molecular mass of 56 kDa. Sequence anaylsis showed high homology compared to other plant MIPS genes, because it contained typical domains owned by MIPS enzymes. The transcript levels of the rcMIPS gene in leaves, stems, and roots were examined after drought stress for 24, 48, and 72 h. The transcript levels in the leaves, stems, and roots increased significantly compared to the control. Results of the enzyme assay showed a significant correlation between the changes of enzyme activity and the transcript levels of the rcMIPS gene in different organs. Decreased relative water contents (RWC) and increased malondialdehyde (MDA) contents in the leaves represented a stress response against drought stress. Our findings suggest that MIPS plays an important role in the defensive mechanisms of R. communis against drought stress.

Use of remote sensing for drought stress monitoring, yield prediction and varietal evaluation in castor beans (Ricinus communis L.)

2005 ◽  
Vol 26 (24) ◽  
pp. 5525-5534 ◽  
Author(s):  
P. Vijaya Kumar ◽  
Y. S. Ramakrishna ◽  
D. V. Bhaskara Rao ◽  
G. Sridhar ◽  
G. Srinivasa Rao ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Drought Stress ◽  
Ricinus Communis ◽  
Yield Prediction ◽  
Stress Monitoring ◽  
Ricinus Communis L

Identification of reference genes for quantitative RT-PCR analysis of microRNAs and mRNAs in castor bean (Ricinus communis L.) under drought stress

2016 ◽  
Vol 106 ◽  
pp. 101-107 ◽  
Author(s):  
Daniela Cassol ◽  
Fernanda P. Cruz ◽  
Kauê Espindola ◽  
Amanda Mangeon ◽  
Caroline Müller ◽  
...  
Keyword(s):  
Drought Stress ◽  
Reference Genes ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Ricinus Communis L

Pressure–volume curves of shoots and roots of normal and drought conditioned western hemlock seedlings

1980 ◽  
Vol 10 (1) ◽  
pp. 10-16 ◽  
Author(s):  
R. A. Kandiko ◽  
R. Timmis ◽  
J. Worrall
Keyword(s):  
Drought Stress ◽  
Water Content ◽  
Water Deficit ◽  
Western Hemlock ◽  
Relative Water ◽  
Osmotic Potentials ◽  
Water Contents

Pressure–volume curves for western hemlock (Tsugaheterophylla (Raf.) Sarg.) seedlings showed roots to have lower osmotic potentials, at both full turgor and incipient plasmolysis, and lower relative water contents at incipient plasmolysis than shoots. Roots remained turgid under mild water deficit, whereas shoots lost turgor exponentially with water content. Shoots of seedlings given a 2-week drought stress had lower osmotic potentials at full turgor and incipient plasmolysis than shoots of unstressed seedlings; roots showed a similar trend.

New insights into the mechanism underlying Ricinus communis L. tolerance to drought stress during germination

2017 ◽  
Vol 103 ◽  
pp. 99-106 ◽  
Author(s):  
Paulo de Carvalho Teixeira Vasconcelos ◽  
Marta Bruno Loureiro ◽  
Águeda Maria Magalhães Fraga Lima ◽  
Paulo Roberto Ribeiro ◽  
Daniele Takahashi Bernal ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ricinus Communis ◽  
Ricinus Communis L

scholarly journals Photosynthetic capacity and water use efficiency in Ricinus communis (L.) under drought stress in semi-humid and semi-arid areas

2017 ◽  
Vol 89 (4) ◽  
pp. 3015-3029 ◽  
Author(s):  
CLAUDIANA M. DOS SANTOS ◽  
LAURÍCIO ENDRES ◽  
VILMA M. FERREIRA ◽  
JOSÉ V. SILVA ◽  
EDUARDO V. ROLIM ◽  
...  
Keyword(s):  
Drought Stress ◽  
Water Use Efficiency ◽  
Water Use ◽  
Photosynthetic Capacity ◽  
Ricinus Communis ◽  
Arid Areas ◽  
Ricinus Communis L ◽  
Use Efficiency ◽  
Semi Arid

sll1722, an unassigned open reading frame of Synechocystis PCC�6803, codes for l- myo -inositol 1-phosphate synthase

Planta ◽  
2004 ◽  
Vol 218 (6) ◽  
pp. 989-998 ◽  
Author(s):  
Anirban Chatterjee ◽  
Manoj Majee ◽  
Shilpi Ghosh ◽  
Arun Lahiri Majumder
Keyword(s):  
Open Reading Frame ◽  
Synechocystis Pcc 6803 ◽  
Reading Frame ◽  
Inositol 1 ◽  
Pcc 6803 ◽  
Phosphate Synthase

scholarly journals Evaluation of Drought Stress and Foliar Chitosan on Biochemical Characterices of Castor Bean (Ricinus communis L.)

2012 ◽  
Vol 7 (3) ◽  
pp. 117-122 ◽  
Author(s):  
Sara Karimi ◽  
Hossein Abbaspour ◽  
Jafar Masoud Sin ◽  
Hassan Makarian
Keyword(s):  
Drought Stress ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Ricinus Communis L

scholarly journals Silencing MdGH3-2/12 in apple reduces drought resistance by regulating AM colonization

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dong Huang ◽  
Qian Wang ◽  
Zhijun Zhang ◽  
Guangquan Jing ◽  
Mengnan Ma ◽  
...  
Keyword(s):  
Drought Stress ◽  
Mycorrhizal Fungi ◽  
Crop Yields ◽  
Negative Impact ◽  
Arbuscular Mycorrhizal ◽  
Ros Scavenging ◽  
Crop Species ◽  
Relative Water ◽  
Am Symbiosis ◽  
Water Contents

AbstractDrought leads to reductions in plant growth and crop yields. Arbuscular mycorrhizal fungi (AMF), which form symbioses with the roots of the most important crop species, alleviate drought stress in plants. In the present work, we identified 14 GH3 genes in apple (Malus domestica) and provided evidence that MdGH3-2 and MdGH3-12 play important roles during AM symbiosis. The expression of both MdGH3-2 and MdGH3-12 was upregulated during mycorrhization, and the silencing of MdGH3-2/12 had a negative impact on AM colonization. MdGH3-2/12 silencing resulted in the downregulation of five genes involved in strigolactone synthesis, and there was a corresponding change in root strigolactone content. Furthermore, we observed lower root dry weights in RNAi lines under AM inoculation conditions. Mycorrhizal transgenic plants showed greater sensitivity to drought stress than WT, as indicated by their higher relative electrolytic leakage and lower relative water contents, osmotic adjustment ability, ROS scavenging ability, photosynthetic capacity, chlorophyll fluorescence values, and abscisic acid contents. Taken together, these data demonstrate that MdGH3-2/12 plays an important role in AM symbiosis and drought stress tolerance in apple.

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