Pressure–volume curves of shoots and roots of normal and drought conditioned western hemlock seedlings

1980 ◽  
Vol 10 (1) ◽  
pp. 10-16 ◽  
Author(s):  
R. A. Kandiko ◽  
R. Timmis ◽  
J. Worrall

Pressure–volume curves for western hemlock (Tsugaheterophylla (Raf.) Sarg.) seedlings showed roots to have lower osmotic potentials, at both full turgor and incipient plasmolysis, and lower relative water contents at incipient plasmolysis than shoots. Roots remained turgid under mild water deficit, whereas shoots lost turgor exponentially with water content. Shoots of seedlings given a 2-week drought stress had lower osmotic potentials at full turgor and incipient plasmolysis than shoots of unstressed seedlings; roots showed a similar trend.

2021 ◽  
Vol 13 (5) ◽  
pp. 2923
Author(s):  
Botir Khaitov ◽  
Munisa Urmonova ◽  
Aziz Karimov ◽  
Botirjon Sulaymonov ◽  
Kholik Allanov ◽  
...  

Water deficiency restricts plant productivity, while excessive soil moisture may also have an adverse impact. In light of this background, field trials were conducted in secondary saline soil (EC 6.5 dS m−1) at the experimental station of Tashkent State Agrarian University (TSAU), Uzbekistan to determine drought tolerance of licorice (Glycyrrhiza glabra) by exposure to four levels of water deficit, namely control (70–80%), moderate (50–60%), strong (30–40%) and intense (10–20%) relative water content (WC) in the soil. The moderate drought stress exhibited positive effects on the morphological and physiological parameters of licorice, and was considered to be the most suitable water regime for licorice cultivation. Plant growth under the 50–60% WC treatment was slightly higher as compared to 70–80% WC treatment, exhibiting weak water deficit promotes licorice growth, root yield and secondary metabolite production. In particular, secondary metabolites i.e., ash, glycyrrhizic acid, extractive compounds and flavonoids, tended to increase under moderate water deficit, however further drought intensification brought a sharp decline of these values. These results contribute to the development of licorice cultivation technologies in arid regions and the most important consideration is the restoration of ecological and economical functions of the dryland agricultural system.


1997 ◽  
Vol 24 (1) ◽  
pp. 19-24 ◽  
Author(s):  
P. J. Sexton ◽  
J. M. Bennett ◽  
K. J. Boote

Abstract Peanut (Arachis hypogaea L.) fruit growth is sensitive to surface soil (0-5 cm) conditions due to its subterranean fruiting habit. This study was conducted to determine the effect of soil water content in the pegging zone (0-5 cm) on peanut pod growth rate and development. A pegging-pan-root-tube apparatus was used to separately control soil water content in the pegging and root zone for greenhouse trials. A field study also was conducted using portable rainout shelters to create a soil water deficit. Pod phenology, pod and seed growth rates, and final pod and seed dry weights were determined. In greenhouse studies, dry pegging zone soil delayed pod and seed development. In the field, soil water deficits in the pegging and root zone decreased pod and seed growth rates by approximately 30% and decreased weight per seed from 563 to 428 mg. Pegs initiating growth during drought stress demonstrated an ability to suspend development during the period of soil water deficit and to re-initiate pod development after the drought stress was relieved.


Genetika ◽  
2012 ◽  
Vol 44 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Ahmad Golparvar

Mode of gene action, heritability and determination of the effective breeding strategy for improvement of physiological and traits specifically in drought stress conditions is very important. Therefore, this study was conducted by using two drought susceptible and tolerant wheat cultivars. Cultivars Sakha8 (tolerant) and Pishtaz (susceptible) as parents along with F1, F2, BC1 and BC2 generations were sown in a randomized complete block design with three replications in drought stress conditions. Results of analysis of variance indicated significant difference between generations as well as degree of dominance revealed over-dominance for the both traits. Fitting simple additive-dominance model designated that this model was not able to account for changes of traits relative water content and mean of grain filling rate. It was revealed that m-d-h-i-j model for relative water content and m-d-h-i model for mean of grain filling rate are the best models. Estimation of heritability and mode of gene action indicated that selection for improvement of traits studied in stress condition and specifically in early generations have medium genetic gain. In conclusion, grain filling rate is better than relative water content as indirect selection criteria to improve plant grain yield in drought stress condition.


2019 ◽  
Vol 18 (6) ◽  
pp. 75-84
Author(s):  
Alireza Motallebi-Azar ◽  
István Papp ◽  
Anita Szegő

Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial variety Honeydew. Plants were treated with drought stress by adjusting the soil water content to 75, 50, 40, 30 and 20% of field capacity (FC) by withholding water. Water status of plants was monitored based on the seedling fresh weight (FW) and relative water content of leaves (RWC). Total protein content was extracted, then heat-stable protein (HSP) fraction was isolated for each variety and water stress treatment. After SDS-PAGE of HSP, Western blotting analysis was carried out with Anti-dehydrin rabbit (primary) and Goat anti rabbit (secondary) antibodies. ANOVA results showed that with decreasing FC below 75%, FW and RWC decreased, but these changes significantly varied among genotypes. On the basis of FW and RWC data under different drought stress treatments, the following drought-tolerant ranking was established: Mino > Dargazi > Saveii and Honeydew > Semsori, from tolerant to sensitive order. Results of Western blot analysis showed that expression of some proteins with molecular weights of 19–52 kDa was induced in the studied varieties under drought stress (% FC). Expression level of the dehydrin proteins in different varieties was variable and also depending on the drought stress level applied. However, dehydrin proteins (45 and 50 kDa) showed strong expression levels in all varieties under severe drought stress (20% FC). The abundance of dehydrin proteins was higher in tolerant varieties (Mino and Dargazi) than in moderate and drought sensitive genotypes. Consequently, dehydrins represent a potential marker for selection of genotypes with enhanced drought tolerance.


2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016


2020 ◽  
Vol 48 (1) ◽  
pp. 234-244
Author(s):  
Edinéia M.M. BARTIERES ◽  
Silvana P.Q. SCALON ◽  
Daiane M. DRESCH ◽  
Edvânia A.S. CARDOSO ◽  
Mailson V. JESUS ◽  
...  

In this research it was hypothesized that Campomanesia xanthocarpa can overcome some level of water deficiency by adjusting physiological parameters and that shading minimizes the water deficit effects while maintaining elevated photosynthetic rates and relative water content of the leaves and makes a resumption of metabolism and growth when the water supply is normalized. The seedlings were submitted to two water regimes (continuous irrigation - CI and intermittent irrigation - II), three shading percentages (0, 30 and 70%) and six evaluation times (Start - T0, 1st Photosynthesis Zero - 1st P0, 1st Recovery - 1st REC, 2nd Photosynthesis Zero - 2nd P0, 2nd Recovery - 2nd REC and END). Plants under water deficit at 0% shading led to a reduction in photosynthetic metabolism, relative water content (RWC), leaf area, number of leaves, and height, especially during the stress periods 1st and 2nd P0. The 30 and 70% shading mitigated the stressful effect of water deficit on C. xanthocarpa seedlings. The results did not confirm the hypothesis that C. xanthocarpa seedlings are intolerant to water deficit since, although sensitive, they presented a potential for recovery of photosynthetic and growth characteristics under all cultivation conditions. It was concluded that that shading minimizes the stressful effects of water deficit.


2020 ◽  
Vol 71 (9) ◽  
pp. 2713-2722 ◽  
Author(s):  
Haicui Xie ◽  
Jianqin Shi ◽  
Fengyu Shi ◽  
Haiyun Xu ◽  
Kanglai He ◽  
...  

Abstract Plants are routinely subjected simultaneously to different abiotic and biotic stresses, such as heat, drought, and insect infestation. Plant–insect interactions in such complex stress situations are poorly understood. We evaluated the performance of the grain aphid (Sitobion avenae) in wheat (Triticum aestivum L.) exposed to a combination of heat and drought stresses. We also performed assays of the relative water content, nutritional quality, and responses of phytohormone signaling pathways. Lower relative water content and accumulation of soluble sugars and amino acids were observed in plants exposed to combined heat and drought stress. These conditions increased abscisic acid levels in the absence of aphids, as well as leading to higher levels of jasmonate-dependent transcripts. The grain aphid infestation further increased abscisic acid levels and the abundance of jasmonic acid- and salicylic acid-dependent defenses under the combined stress conditions. Aphids reared on plants grown under drought stress alone showed lower net reproductive rates, intrinsic rates of increase, and finite rates of increase compared with aphids reared on plants in the absence of stress. The heat-treated plants also showed a decreased aphid net reproductive rate. These findings demonstrate that exposure to a combination of stresses enhances plant defense responses against aphids as well as altering nutritional quality.


2016 ◽  
Vol 75 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Zamin Shaheed Siddiqui ◽  
Huda Shahid ◽  
Jung-Il Cho ◽  
Sung-Han Park ◽  
Tae-Hun Ryu ◽  
...  

AbstractThe physiological responses of two halophytic grass species, Halopyrum mucronatum (L.) Staph. and Cenchrus ciliaris (L.), under drought stress were evaluated. Biomass accumulation, relative water content, free proline, H2O2content, stomatal conductance, photosynthetic performance and quantum yield (Fv/Fmratio) were studied. Under drought conditions, these halophytic plants expressed differential responses to water deficit. Stomatal conductance and free proline content were higher in H. mucronatum than in C. ciliaris, while H2O2content in H. mucronatum was substantially lower than in C. ciliaris. Performance index showed considerable sensitivity to a water deficit condition, more so in C. ciliaris than in H. mucronatum. Results were discussed in relation to comparative physiological performance and antioxidant enzymes activity of both halophytic grasses under drought stress.


Author(s):  
K.D. Nkoana ◽  
Abe Shegro Gerrano ◽  
E.T. Gwata

The genetic potential for drought tolerance in cowpea within the small holder sector has not been fully exploited in South Africa. Thus, a drought evaluation experiment was conducted at the ARC-VOP to evaluate 28 cowpea germplasm accessions including two controls viz. IT96D-602 (drought tolerant) and TVU7778 (susceptible to drought) in the drought screening house using plastic box evaluation method in January, 2017. Genotypes raised for three weeks were subjected to 5 weeks of water stress treatment to determine their physiological response through leaf wilting index, relative water content and proline content followed by re-watering to determine genotype (s) with ability to recover from drought stress. Analyses of variance showed highly significant differences in response to moisture stress among the cowpea accessions for the selected physiological traits except for leaf wilting index at week two of drought stress. Stem greenness and recovery appeared to be a reliable indicator of drought tolerant genotypes which was readily observed in Acc1257, Acc1168, Acc2355, IT96D-602 and Acc5352 which also correlated significantly and positively with relative water content and proline content. The genotypes responded differently to drought stress indicating that there is sufficient genetic variability that can be utilized further in breeding for drought stress within the cowpea species.


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