scholarly journals Axonal signals regulate expression of glia maturation factor-beta in Schwann cells: an immunohistochemical study of injured sciatic nerves and cultured Schwann cells

1989 ◽  
Vol 9 (10) ◽  
pp. 3690-3698 ◽  
Author(s):  
EP Bosch ◽  
W Zhong ◽  
R Lim
Keyword(s):  
Schwann Cells ◽  
Immunohistochemical Study ◽  
Glia Maturation Factor ◽  
Maturation Factor ◽  
Cultured Schwann Cells ◽  
Sciatic Nerves

Glia maturation factor promotes proliferation and morphologic expression of rat Schwann cells

1984 ◽  
Vol 304 (2) ◽  
pp. 311-319 ◽  
Author(s):  
E. Peter Bosch ◽  
Jose G. Assouline ◽  
Joyce F. Miller ◽  
Ramon Lim
Keyword(s):  
Schwann Cells ◽  
Glia Maturation Factor ◽  
Maturation Factor

Endogenous immunoreactive glia maturation factor-like molecule in cultured rat Schwann cells

1988 ◽  
Vol 40 (2) ◽  
pp. 277-284 ◽  
Author(s):  
Ramon Lim ◽  
Danny J. Hicklin ◽  
Timothy C. Ryken ◽  
Joyce F. Miller ◽  
E.Peter Bosch
Keyword(s):  
Schwann Cells ◽  
Glia Maturation Factor ◽  
Maturation Factor

Connexin32 Gene Expression in Rat Sciatic Nerves and Cultured Schwann Cells

1997 ◽  
Vol 19 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Marie Satake ◽  
Takeo Yoshimura ◽  
Akio Ohnishi ◽  
Takuro Kobayashi
Keyword(s):  
Gene Expression ◽  
Schwann Cells ◽  
Cultured Schwann Cells ◽  
Sciatic Nerves

Dual effect of glia maturation factor on astrocytes

1983 ◽  
Vol 5 (3) ◽  
pp. 261-269 ◽  
Author(s):  
A. Fontana ◽  
E. Weber ◽  
P.J. Grob ◽  
R. Lim ◽  
J.F. Miller
Keyword(s):  
Glia Maturation Factor ◽  
Dual Effect ◽  
Maturation Factor

scholarly journals Glia maturation factor-γ regulates murine macrophage iron metabolism and M2 polarization through mitochondrial ROS

2019 ◽  
Vol 3 (8) ◽  
pp. 1211-1225 ◽  
Author(s):  
Wulin Aerbajinai ◽  
Manik C. Ghosh ◽  
Jie Liu ◽  
Chutima Kumkhaek ◽  
Jianqing Zhu ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Mitochondrial Function ◽  
Regulatory Protein ◽  
M2 Macrophage ◽  
Macrophage Phenotype ◽  
Glia Maturation Factor ◽  
Altered Expression ◽  
Murine Macrophages ◽  
Cellular Iron ◽  
Maturation Factor

Abstract In macrophages, cellular iron metabolism status is tightly integrated with macrophage phenotype and associated with mitochondrial function. However, how molecular events regulate mitochondrial activity to integrate regulation of iron metabolism and macrophage phenotype remains unclear. Here, we explored the important role of the actin-regulatory protein glia maturation factor-γ (GMFG) in the regulation of cellular iron metabolism and macrophage phenotype. We found that GMFG was downregulated in murine macrophages by exposure to iron and hydrogen peroxide. GMFG knockdown altered the expression of iron metabolism proteins and increased iron levels in murine macrophages and concomitantly promoted their polarization toward an anti-inflammatory M2 phenotype. GMFG-knockdown macrophages exhibited moderately increased levels of mitochondrial reactive oxygen species (mtROS), which were accompanied by decreased expression of some mitochondrial respiration chain components, including the iron-sulfur cluster assembly scaffold protein ISCU as well as the antioxidant enzymes SOD1 and SOD2. Importantly, treatment of GMFG-knockdown macrophages with the antioxidant N-acetylcysteine reversed the altered expression of iron metabolism proteins and significantly inhibited the enhanced gene expression of M2 macrophage markers, suggesting that mtROS is mechanistically linked to cellular iron metabolism and macrophage phenotype. Finally, GMFG interacted with the mitochondrial membrane ATPase ATAD3A, suggesting that GMFG knockdown–induced mtROS production might be attributed to alteration of mitochondrial function in macrophages. Our findings suggest that GMFG is an important regulator in cellular iron metabolism and macrophage phenotype and could be a novel therapeutic target for modulating macrophage function in immune and metabolic disorders.

scholarly journals Antiproliferative function of glia maturation factor beta.

1990 ◽  
Vol 1 (10) ◽  
pp. 741-746 ◽  
Author(s):  
R Lim ◽  
W X Zhong ◽  
A Zaheer
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Target Cell ◽  
G1 Phase ◽  
Glia Maturation Factor ◽  
Pituitary Extract ◽  
Mitogenic Effect ◽  
Maturation Factor ◽  
Cultured Astrocytes

Recombinant human glia maturation factor beta (GMF-beta) reversibly inhibits the proliferation of neoplastic cells in culture by arresting the cells in the G0/G1 phase. This phenomenon is not target-cell specific, as neural and nonneural cells are equally inhibited. When tested simultaneously, GMF-beta suppresses the mitogenic effect of acidic fibroblasts growth factor (aFGF), but the two are synergistic in promoting the morphologic differentiation of cultured astrocytes. GMF-beta also counteracts the growth-stimulating effect of pituitary extract and cholera toxin on Schwann cells. The results underscore the regulatory role of GMF-beta and its intricate interaction with the mitogenic growth factors.

Purification of bovine glia maturation factor and characterization with monoclonal antibody

Biochemistry ◽  
1985 ◽  
Vol 24 (27) ◽  
pp. 8070-8074 ◽  
Author(s):  
Ramon Lim ◽  
Joyce F. Miller ◽  
Danny J. Hicklin ◽  
Andrew A. Andresen
Keyword(s):  
Monoclonal Antibody ◽  
Glia Maturation Factor ◽  
Maturation Factor
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