ABRUPTIO PLACENTAE IN VITAMIN E DEFICIENT GUINEA PIGS

Myoglobin concentration was determined in gastrocnemius and masseter muscles of guinea pigs maintained up to 15 days on vitamin E-deficient and vitamin E-supplemented diets. A statistically significant increase in myoglobin was noted in muscles of animals on the deficient diet for 15 days. That the increase was real and not apparent was attested by studies of total nitrogen, noncollagen nitrogen, percentage of solids and muscle wet weight, all of which were the same in control and experimental muscles. Histological sections and creatine excretion studies confirmed the impression of mild, incipient nutritional dystrophy.

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Some Observations on the Genital Function of Vitamin E-Deficient Female Non-Pregnant Guinea-Pigs

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.3109/00016345809157441 ◽

1958 ◽

Vol 37 (3) ◽

pp. 358-364

Author(s):

Axel Ingelman-Sundberg

Keyword(s):

Vitamin E ◽

Guinea Pigs

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The effect of vitamin E on lung pathology in sulfur mustard-exposed guinea pigs

Toxicology and Industrial Health ◽

10.1177/0748233715600986 ◽

2016 ◽

Vol 32 (12) ◽

pp. 1971-1977 ◽

Cited By ~ 6

Author(s):

Zahra Gholamnezhad ◽

Mohammad Hossein Boskabady ◽

Sediqa Amery ◽

Nassim Vahedi ◽

Abass Tabatabaei ◽

...

Keyword(s):

Vitamin E ◽

Guinea Pigs ◽

Lung Inflammation ◽

Sulfur Mustard ◽

Pulmonary Complications ◽

Control Group ◽

Lung Pathology ◽

Pathological Changes ◽

Bronchial Stenosis ◽

Pathological Evaluation

Pulmonary complications of exposure to sulfur mustard (SM) gas range from no effect or mild symptoms to severe bronchial stenosis. In the present study, the protective effect of vitamin E on the lung inflammation of SM-exposed guinea pigs was examined. Guinea pigs ( n = 5 for each group) were exposed to ethanol (control group), 40 mg/m3 inhaled SM (SME group), SME treated with vitamin E (SME + E), SME treated with dexamethasone (SME + D), and SME treated with both treatments (SME + E + D). Pathological evaluation of the lung was done 14 days postexposure. The epithelial desquamation of trachea and other pathologic changes in the lung of the SME group were significantly higher than those in the control group. Furthermore, the pathological changes of trachea and lung in the SME + E and SME + E + D groups were significantly improved compared with those of SME group. In addition, the pathological changes of trachea and lung of SME + E and SME + E + D animals were significantly less than those of SME + D group.

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Changes in macrophage and lymphocyte functions in guinea-pigs after different amounts of vitamin E ingestion

British Journal Of Nutrition ◽

10.1017/s0007114500001197 ◽

2000 ◽

Vol 84 (1) ◽

pp. 25-29 ◽

Cited By ~ 25

Author(s):

M. De la Fuente ◽

M. Carazo ◽

R. Correa ◽

M. Del Río

Keyword(s):

Immune System ◽

Vitamin E ◽

Guinea Pigs ◽

Superoxide Anion ◽

Superoxide Anion Production ◽

Phagocytic Capacity ◽

High Vitamin ◽

Anion Production ◽

Diet Control ◽

Spleen Lymphocytes

Vitamin E is the main biological lipid-soluble antioxidant and plays a crucial role in the maintenance of the immune system. In the present work, twenty-one guinea-pigs (3-weeks-old) were distributed into three groups, which during 5 weeks ingested different amounts of vitamin E (/kg diet): 15 mg (low vitamin E diet), 150 mg (medium vitamin E diet; control) or 1500 mg (high vitamin E diet). The function of lymphocytes and macrophages were then studied. In macrophages obtained from the peritoneum several steps of the phagocytic process (chemotaxis, ingestion and superoxide anion production) were assayed, as well as chemotaxis and proliferation of peritoneal and spleen lymphocytes. The results indicate that with respect to the medium vitamin E diet, low ingestion of vitamin E causes a decrease in chemotaxis and production of superoxide anion by macrophages and an increase in the phagocytic capacity. With the high vitamin E diet an increase in macrophage and lymphocyte chemotaxis, superoxide anion production and lymphoproliferative capacity, as well as a decrease in phagocytosis, were observed. Therefore, diet supplementation with higher than usual levels of vitamin E appears to be beneficial for the immune system.

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Effect of Prolonged Marginal Ascorbic Acid Deficiency on Lenticular Levels of Antioxidants and Lipid Peroxide in Guinea Pigs

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.71.2.103 ◽

2001 ◽

Vol 71 (2) ◽

pp. 103-109 ◽

Cited By ~ 4

Author(s):

Yoshiji Ohta ◽

Takafumi Niwa ◽

Takashi Yamasaki

Keyword(s):

Ascorbic Acid ◽

Vitamin E ◽

Guinea Pigs ◽

Antioxidant Status ◽

Serum Vitamin ◽

Lipid Peroxide ◽

Ascorbic Acid Content ◽

Ascorbic Acid Deficiency ◽

Acid Deficiency ◽

Deficient Group

We examined the effect of prolonged marginal ascorbic acid deficiency of the levels of antioxidants and lipid peroxide in lenses of guinea pigs in order to clarify lenticular antioxidant status under ascorbic acid deficiency. Male guinea pigs aged 4 weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient ascorbic acid (0.5 mg/animal per day) or sufficient ascorbic acid (1 g/animal per day) in drinking water for 3 and 6 months. The deficient group showed no lens opacity during the administration period. The deficient group had 62.3 and 53.9% of lenticular ascorbic acid content in the sufficient group at 3 and 6 months of ascorbic acid deficiency, respectively. There were no differences in lenticular contents of reduced glutathione and thiobarbituric acid reactive substances, an index of lipid peroxidation, between both groups at 3 and 6 months of ascorbic acid deficiency, while the deficient group tended to have higher lenticular vitamin E content than the sufficient group. The deficient group had higher serum vitamin E concentration than the sufficient group at 3 and 6 months of ascorbic acid deficiency. These results indicate that lenticular antioxidant status is maintained well in guinea pigs with prolonged marginal ascorbic acid deficiency, which may result in no cataract formation.

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Dietary Intervention Accelerates NASH Resolution Depending on Inflammatory Status with Minor Additive Effects on Hepatic Injury by Vitamin E Supplementation

Antioxidants ◽

10.3390/antiox9090808 ◽

2020 ◽

Vol 9 (9) ◽

pp. 808

Author(s):

Julie Hviid Klaebel ◽

Günaj Rakipovski ◽

Birgitte Andersen ◽

Jens Lykkesfeldt ◽

Pernille Tveden-Nyborg

Keyword(s):

Vitamin E ◽

Guinea Pig ◽

Lipid Accumulation ◽

Guinea Pigs ◽

Dietary Intervention ◽

Liver Weight ◽

High Dose ◽

Chow Diet ◽

Lifestyle Modifications ◽

Inflammatory Status

Despite the lack of effective pharmacotherapy against nonalcoholic steatohepatitis (NASH) and liver fibrosis, vitamin E (vitE) supplementation and lifestyle modifications are recommended for the management of NASH due to promising clinical results. We recently reported a positive effect of supplementation with 800 IU vitE and atorvastatin on NASH resolution in guinea pigs. In the present study, we investigated the effect of high-dose vitE therapy combined with dietary intervention against progressive NASH and advanced fibrosis in the guinea pig model. Sixty-six guinea pigs received either high-fat (HF) or standard guinea pig chow diet (Control) for 25 weeks. Prior to eight weeks of intervention, HF animals were allocated into groups; dietary intervention (Chow) or dietary intervention with 2000 IU/d vitE supplementation (CvitE). Both Chow and CvitE reduced dyslipidemia, hepatic lipid accumulation and liver weight (p < 0.05), while CvitE further decreased hepatocellular ballooning (p < 0.05). Subanalyses of individual responses within intervention groups showed significant correlation between the hepatic hallmarks of NASH and lipid accumulation vs. inflammatory state (p < 0.05). Collectively, our results indicate that individual differences in sensitivity towards intervention and inflammatory status determine the potential beneficial effect of dietary intervention and high-dose vitE supplementation. Moreover, the study suggests that inflammation is a primary target in NASH treatment.

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NUTRITIONAL MUSCULAR DYSTROPHY IN THE GUINEA PIG AND RABBIT

Journal of Experimental Medicine ◽

10.1084/jem.54.2.145 ◽

1931 ◽

Vol 54 (2) ◽

pp. 145-165 ◽

Cited By ~ 92

Author(s):

Marianne Goettsch ◽

Alwin M. Pappenheimer

Keyword(s):

Vitamin E ◽

Muscular Dystrophy ◽

Guinea Pig ◽

Guinea Pigs ◽

Unknown Factor ◽

Nutritional Muscular Dystrophy

A diet is described, which leads to a progressive, highly selective, and ultimately fatal dystrophy of the voluntary muscles. Guinea pigs and rabbits are susceptible, rats resistant. The diet used is complete in known requirements, except for vitamin E; the addition of this factor, however, does not prevent the development of the disease. The lesions are not due to inanition, infection, or scurvy, and must be referred to some still unknown factor.

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