THE UPTAKE OF 131I TRIIODOTHYRONINE BY RESIN. AN IN VITRO TEST OF THYROID FUNCTION

1961 ◽  
Vol 37 (4) ◽  
pp. 607-615 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos
Keyword(s):  
Thyroid Function ◽  
Plasma Sample ◽  
In Vitro Test ◽  
Red Cell ◽  
Normal Range ◽  
Cell Substrate ◽  
Vitro Test ◽  
Uptake Test ◽  
Hyperthyroid Patients

ABSTRACT A technique is described, which resembles the in vitro red cell triiodothyronine uptake test for the investigation of thyroid function, but which requires only 0.5 ml of plasma. The uptake of radio-triiodothyronine is measured by a method in which resin replaces the erythrocytes. Thus the variable of the red cell substrate is excluded. By means of the resin technique described, an uptake of 14–27 % was found in a group of 130 euthyroid subjects. Fourteen plasma samples from hyperthyroid patients were investigated, which showed an uptake range of 25–40 % (one plasma sample from this group had an uptake of 25.8, i. e. within the normal range). In one case of hypothyroidism, an uptake of 10.7 % was found. It is not yet possible to state the level of uptake below which the diagnosis of hypothyroidism should be considered. The effect of different factors like the quantity of resin, incubationtemperature and incubation-time, pH and others have been investigated. The results are discussed.

THE "EFFECTIVE THYROXINE RATIO" – NEW IN VITRO TEST OF THYROID FUNCTION AND ITS CORRELATION WITH FREE THYROXINE

1974 ◽  
Vol 76 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Janusz Nauman ◽  
Alicja Nauman
Keyword(s):  
Pregnant Women ◽  
Normal Subjects ◽  
Free Thyroxine ◽  
In Vitro Test ◽  
High Reproducibility ◽  
Vitro Test ◽  
The Mean ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The effective thyroxine ratio (ETR) and absolute concentration of free thyroxine (AFT4) were estimated in the sera of 31 normal subjects, 27 hyperthyroid patients, 12 hypothyroid patients and 21 euthyroid pregnant women. The mean ETR value in the controls was 1.0 ± 0.18, in the hyperthyroid patients 1.31 ± 0.25, in the hypothyroid patients 0.71 ± 0.21 and in normal pregnant women 0.99 ± 0.24. The mean AFT4 in the normal subjects was 3.0 ± 0.53 ng/100 ml, in the hyperthyroid patients 9.49 ± 2.44 ng/ 100 ml, in the hypothyroid patients 0.58 ± 0.15 ng/100 ml and in the pregnant women 2.84 ± 0.63 ng/100 ml, respectively. High reproducibility of ETR and a significant positive correlation between ETR and AFT4 with r = 0.96 suggest that ETR might be a suitable in vitro test for routine clinical evaluation of the thyrometabolic state.

scholarly journals Res-O-Mat T3 as an in vitro Test of Thyroid Function

RADIOISOTOPES ◽  
1970 ◽  
Vol 19 (3) ◽  
pp. 136-141
Author(s):  
Hirofumi MORI ◽  
Tsutomu MISHIMA ◽  
Kin-ichi HISADA
Keyword(s):  
Thyroid Function ◽  
In Vitro Test ◽  
Vitro Test

Lack of Influence of Povidone-Iodine on Tests of Thyroid Function

1978 ◽  
Vol 6 (5) ◽  
pp. 406-408 ◽  
Author(s):  
D P Lividas ◽  
G D Piperingos ◽  
J Sfontouris ◽  
D A Koutras
Keyword(s):  
Thyroid Function ◽  
Povidone Iodine ◽  
Test Methods ◽  
Thyroid Function Tests ◽  
In Vitro Test ◽  
External Application ◽  
Vitro Test ◽  
Antiseptic Agent

The external application of povidone-iodine, an antiseptic agent, was tested for its influence on thyroid function. Previous workers have described some in vitro changes in thyroid function tests following its use. In the present study topical application of povidone-iodine did not affect thyroid function as measured some days later using both in vivo and radio-active iodine in vitro test methods, despite the fact that the latter are notorious for being influenced by exogenous iodine.

EVALUATION OF THYROID STATUS WITH THE "EFFECTIVE THYROXINE RATIO" (E. T. R.®)

1974 ◽  
Vol 77 (2) ◽  
pp. 250-256 ◽  
Author(s):  
Klaus Kølendorf ◽  
Kaj Siersbæk-Nielsen ◽  
Jens Mølholm Hansen ◽  
Thorkild Friis
Keyword(s):  
Thyroid Function ◽  
Thyroid Function Test ◽  
Free Thyroxine ◽  
Normal Range ◽  
High Positive Correlation ◽  
Function Test ◽  
Free Thyroxine Index ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT A new in vitro thyroid function test, "Effective Thyroxine Ratio" (ETR®) has been tested in 170 patients, and the ETR values in patients with normal and abnormal thyroid function have been compared to the concentration of absolute free thyroxine and free thyroxine index in serum. Among 42 hyperthyroid patients tested with ETR, 13 had values within normal range (30.9 per cent). Three of 18 hypothyroid patients had normal values (16.8 per cent). Eighty-nine per cent of 27 pregnant women and all of 17 patients treated with genuine oestrogens had ETR values within normal range. A high positive correlation was found between values of ETR, absolute free thyroxine in serum (r = 0.62) and free thyroxine index (r = 0.83).

THE BINDING OF 131I-TRIIODOTHYRONINE BY SERUM PROTEINS AS AN IN VITRO TEST OF THYROID FUNCTION

1964 ◽  
Vol 46 (2) ◽  
pp. 161-169 ◽  
Author(s):  
A. Cuarón ◽  
M. E. Fucugauchi
Keyword(s):  
Thyroid Function ◽  
Screening Test ◽  
Serum Proteins ◽  
In Vitro Test ◽  
Radiation Hazards ◽  
Clinical Screening ◽  
Sephadex Column ◽  
Vitro Test ◽  
High Degree

ABSTRACT The efficiency of Sephadex column chromatography for the separation of free iodide, free T3, and protein bound T3 has been demonstrated. Protein bound T3 and free iodide appear as two well defined peaks of activity in the eluate, while free T3 remains bound to the Sephadex column. This methods makes the direct study of the binding of T3 to serum proteins possible and allows of correction for the free radioactive iodide present as a contaminant. The value of this method in 147 selected patients was demonstrated by the high degree of accuracy in the diagnosis of thyroid function. The clinical diagnosis was confirmed by this method in 141 patients (95.9%) and the test failed in 6 cases (4.1%). The use of this method as a clinical screening test of thyroid function is suggested as it is sufficiently reliable, simple to perform and does not expose the patient to radiation hazards.

The charcoal T3 ratio. An in vitro test of thyroid function

JAMA ◽  
1967 ◽  
Vol 199 (7) ◽  
pp. 469-472 ◽  
Author(s):  
L. E. Braverman
Keyword(s):  
Thyroid Function ◽  
In Vitro Test ◽  
Vitro Test

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

IN VITRO UPTAKE OF RADIOACTIVE 131I LABELLED L-TRIIODOTHYRONINE BY HUMAN ERYTHROCYTES AS AN INDEX OF THYROID FUNCTION

1960 ◽  
Vol XXXIV (II) ◽  
pp. 305-311 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos
Keyword(s):  
Thyroid Function ◽  
Incubation Time ◽  
Clinical Results ◽  
Human Erythrocytes ◽  
Red Cell ◽  
Clinical Value ◽  
Blood Samples ◽  
In Vitro Uptake

ABSTRACT The red cell triiodothyronine uptake technique as used in our hospital is described. Incubation time is of almost no importance. The temperature during incubation should be 37° C. Further improvement of the technique is obtained when all blood samples are brought up to 40 % haematocrit prior to incubation. Clinical results are discussed. It is yet too early to give a definite assessment of its clinical value, but it is definitely superior to the measurement of the BMR.

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