A MODIFIED METHOD FOR THE QUANTITATIVE DETERMINATION OF PROGESTERONE IN HUMAN PLASMA

1963 ◽  
Vol 43 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Ian F. Sommerville ◽  
Mary T. Pickett ◽  
William P. Collins ◽  
Diane C. Denyer
Keyword(s):  
Menstrual Cycle ◽  
Quantitative Determination ◽  
Human Plasma ◽  
Peripheral Blood ◽  
Luteal Phase ◽  
Chemical Method ◽  
Blood Levels ◽  
Modified Method

ABSTRACT A more sensitive and less complicated chemical method has been evolved for the quantitative determination of progesterone in 5–10 ml samples of human plasma. The method is suitable for application to peripheral blood levels at all stages of pregnancy and may be applied to the study of the luteal phase of the menstrual cycle. The sensitivity, accuracy, precision and specificity of the method are discussed.

THE FLUORIMETRIC DETERMINATION OF PROGESTERONE IN HUMAN PLASMA DURING PREGNANCY AND THE MENSTRUAL CYCLE

1962 ◽  
Vol 25 (2) ◽  
pp. 239-244 ◽  
Author(s):  
R. V. SHORT ◽  
IRIS LEVETT
Keyword(s):  
Menstrual Cycle ◽  
Pregnant Women ◽  
Human Plasma ◽  
Peripheral Blood ◽  
Luteal Phase ◽  
Follicular Phase ◽  
Fluorimetric Determination ◽  
Fluorescence Reaction ◽  
Good Agreement

SUMMARY The fluorescence reaction for progesterone described by Touchstone & Murawec (1960) has been used to determine the concentration of progesterone in nineteen samples of peripheral blood from pregnant women, and in seventeen samples of peripheral blood from women during the course of the menstrual cycle. There was good agreement between the ultraviolet and fluorescent estimates of progesterone in all the samples from pregnant women. The concentrations found during the follicular phase of the menstrual cycle were in general lower than those found during the luteal phase. In one woman who was sampled repeatedly during the course of a menstrual cycle, there was a well defined rise in the level of progesterone in the blood after the expected date of ovulation.

Quantitative Determination of Progesterone in Human Plasma by Thin-Layer and Gas-Liquid Radiochromatography

Nature ◽  
1964 ◽  
Vol 203 (4947) ◽  
pp. 836-839 ◽  
Author(s):  
WILLIAM P. COLLINS ◽  
IAN F. SOMMERVILLE
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Human Plasma

scholarly journals A Modified Method for Determination of Lumefantrine in Human Plasma by HPLC-UV and Combination of Protein Precipitation and Solid-Phase Extraction: Application to a Pharmacokinetic Study

2010 ◽  
Vol 5 ◽  
pp. ACI.S4431 ◽  
Author(s):  
Liusheng Huang ◽  
Patricia S. Lizak ◽  
Anura L. Jayewardene ◽  
Florence Marzan ◽  
Ming-Na Tina Lee ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Gradient Elution ◽  
Protein Precipitation ◽  
Phase Extraction ◽  
Modified Method

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C8 cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3.0 × 150 mm, 3.5 μm) with water/methanol (0.1% TFA) as the mobile phases in a gradient elution mode. Detection was performed using UV/vis detector at λ = 335 nm. The method showed to be linear over a range of 50-10,000 ng/mL with acceptable intra- and inter-day precision and accuracy. The mean recoveries were 88.2% for lumefatrine and 84.5% for the I.S. The internal standard halofantrine is readily available from commercial sources. This method was successfully applied to a pharmacokinetic interaction study between a first-line antimalarial combination (artemether—lumefantrine) and antiretroviral therapy.

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