USE OF HPLC TO DETERMINE THE EFFECT OF 17β-HYDROXY-7α-METHYLANDROST-5-EN-3-ONE (RMI 12,936) ON PRODUCTION OF PROGESTERONE BY RAT OVARIAN HOMOGENATE

1978 ◽  
Vol 88 (1) ◽  
pp. 157-163 ◽  
Author(s):  
R. B. Taylor ◽  
K. E. Kendle
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Continuous Production ◽  
Hplc Method ◽  
Plasma Progesterone ◽  
Final Level

ABSTRACT A new high pressure liquid chromatography (HPLC) method for the determination of progesterone in the presence of other Δ43-ketosteroids is described. Using this method it is shown that the rate of production of progesterone from pregn-5-ene-3,20-dione by rat ovarian homogenate is initially rapid but falls to zero within 10 min. Experiments indicate that the inhibition is due to the progesterone formed. Inclusion of RMI 12,936 with the pregn-5-ene-3,20-dione substrate results in a lower final level of progesterone and continuous production of 7α-methyltestosterone. The reduction in the levels of progesterone in presence of RMI 12,936 corresponds closely to the reduction in plasma progesterone in vivo following administration of RMI 12,936 described preivously.

scholarly journals Determination of Arylamine N-Acetyltransferase 2 Acetylation Genotype by PCR and Phenotyping Using Dapsone Through High-Pressure Liquid Chromatography Assay: A Gender Wise Study

Dose-Response ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 155932581985553
Author(s):  
Naheed Akhter ◽  
Tahira Iqbal ◽  
Amer Jamil ◽  
Muhammad Akram ◽  
Imtiaz Mehmood Tahir ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Slow Acetylators ◽  
Acetylation Status ◽  
Probe Drug ◽  
Male Volunteers ◽  
Variant Alleles

The main aim of the study was to establish the acetylation status of local population of Pakistan by N-acetyltransferase 2 (NAT2) enzyme and to find out the concordance between phenotypic and genotypic methods for the determination of NAT2 acetylation. Gender-wise comparison of selected healthy male and female volunteers aged greater than 18 years was also conducted to see the effect of sex on NAT2 acetylation. Phenotypically, the rate of acetylation was determined by high-pressure liquid chromatography with dapsone (DDS) probe drug, while genotypically, NAT2 acetylation was determined by using specific primers for NAT2 variant alleles (M1, M2, and M3) amplified in separate polymerase chain reactions. High-pressure liquid chromatography results indicated 64% of the male volunteers to be fast acetylators while 36% were slow acetylators, while ratio of fast and slow acetylators for female was found to be 66% and 34%, respectively. Genotypically, the ratio of fast and slow for male was 60% and 40% and for female was 66% and 34%, respectively. The distribution of 3 NAT2 variant alleles was found in invariable number. For male volunteers, the highest frequency distribution showed by M2 allele was 56%, while for M1 and M3 the frequency was 32% and 12%, respectively, and for female volunteers highest frequency (51%) was shown by the M2 variant allele while lowest frequency (18%) was shown by M3 allele. There was the 94% concordance between the DDS phenotype and genotype. Gender effect on the acetylation was found to be nonsignificant ( P > .05). Therefore, it is concluded that NAT2 acetylation rate can be used to check in vivo acetylation status with dapsone as probe drug. It is concluded that NAT2 acetylation rate was unaffected by gender and can be used to check in vivo acetylation status with dapsone as probe drug, which is inexpensive and less time-consuming.

Evaluation of High Pressure Liquid Chromatography and Gas-Liquid Chromatography for Quantitative Determination of Sugars in Foods

1981 ◽  
Vol 64 (1) ◽  
pp. 139-143
Author(s):  
John L Iverson ◽  
Martin P Bueno
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Hydroxylamine Hydrochloride ◽  
Hplc Method ◽  
Gas Liquid Chromatography ◽  
Processed Foods ◽  
Trimethylsilyl Derivatives ◽  
Liquid Chromatographic

Abstract A method has been developed for separation and determination of 5 sugars in foods. Mixtures of sugars were separated by high pressure liquid chromatography (HPLC) with a μBondapak/carbohydrate column and acetonitrile-water (80 + 20) as the mobile phase. For the gas-liquid chromatographic (GLC) determination, the sugars were reacted with hydroxylamine hydrochloride to give the oximes, which were then converted to trimethylsilyl derivatives. The derivatives were separated on an 8 ft column containing 3% JXR as the liquid phase. The GLC preparative time was considerably longer than that for HPLC. Both methods were applied to the determination of fructose, glucose, sucrose, maltose, and lactose in processed foods. Recovery studies showed better accuracy for the HPLC method. Because of incomplete derivatization and/or the formation of anomers, the GLC method was not applicable to all foods.

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