Evaluation of High Pressure Liquid Chromatography and Gas-Liquid Chromatography for Quantitative Determination of Sugars in Foods

1981 ◽  
Vol 64 (1) ◽  
pp. 139-143
Author(s):  
John L Iverson ◽  
Martin P Bueno
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Hydroxylamine Hydrochloride ◽  
Hplc Method ◽  
Gas Liquid Chromatography ◽  
Processed Foods ◽  
Trimethylsilyl Derivatives ◽  
Liquid Chromatographic

Abstract A method has been developed for separation and determination of 5 sugars in foods. Mixtures of sugars were separated by high pressure liquid chromatography (HPLC) with a μBondapak/carbohydrate column and acetonitrile-water (80 + 20) as the mobile phase. For the gas-liquid chromatographic (GLC) determination, the sugars were reacted with hydroxylamine hydrochloride to give the oximes, which were then converted to trimethylsilyl derivatives. The derivatives were separated on an 8 ft column containing 3% JXR as the liquid phase. The GLC preparative time was considerably longer than that for HPLC. Both methods were applied to the determination of fructose, glucose, sucrose, maltose, and lactose in processed foods. Recovery studies showed better accuracy for the HPLC method. Because of incomplete derivatization and/or the formation of anomers, the GLC method was not applicable to all foods.

Detection of Low-Level Carbadox Residues in Animal Feeds by High Pressure Liquid Chromatography

1977 ◽  
Vol 60 (2) ◽  
pp. 279-283
Author(s):  
Ronald G Luchtefeld
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
False Positive ◽  
Active Drug ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Low Level ◽  
Liquid Chromatographic ◽  
Positive Results

Abstract The high pressure liquid chromatographic (HPLC) method is capable of detecting from 1 to 0.024 ppm methyl 3-(2-quinoxalinyl-methylene) carhazate-Nl,N4-dioxido (carbadox). Carbadox is extracted from the feed with 2% NH4OH in acetone, passed through a liquid-liquid partition, subjected to HPLC, and detected by using a 365 nm detector. No feed materials or other active drug ingredients produced false positive results.

High-Pressure Liquid Chromatography of Benzo (a) pyrene and Benzo(ghi)perylene in Oil-Contaminated Shellfish

1976 ◽  
Vol 59 (5) ◽  
pp. 989-992 ◽  
Author(s):  
Humberto Guerrero ◽  
Edward R Biehl ◽  
Charles T Kenner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Petroleum Ether ◽  
Silica Gel ◽  
High Pressure Liquid Chromatography ◽  
High Pressure Liquid Chromatographic ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
Polynuclear Aromatics

Abstract A high-pressure liquid chromatographic procedure is described for the determination of benzo (a) pyrene and benzo(ghi)perylene. These polynuclear aromatics are extracted with acetonitrile and partitioned into petroleum ether, the petroleum ether is removed, and the residue is saponified. The compounds are purified and isolated by passing the residue through a silica gel column and a high-pressure liquid chromatographic column, and detected by their ultraviolet absorption. Recoveries of standards through the procedure averaged 104%.

High Pressure Liquid Chromatographic Determination of Vitamin D3 in Mineral Feed Premixes

1980 ◽  
Vol 63 (5) ◽  
pp. 1149-1153
Author(s):  
Dennis G Lein ◽  
Harold M Campbell ◽  
Huguette Cohen
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Vitamin D3 ◽  
Ammonium Hydroxide ◽  
Chromatographic Determination ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Better Than

Abstract A simple and rapid quantitative method is described for determining the presence and amount of vitamin D3 (cholecalciferol) in mineral feed premixes by high pressure liquid chromatography. The samples were extracted with dichloromethane–pentane–methanol (46+46+8) after treatment with ammonium hydroxide, cleaned up by silica Sep-pak, and analyzed by normal phase high pressure liquid chromatography: 10 μm LiChrosorb NH2 column, hexane-isopropanol (98+2) mobile phase, ultraviolet detection at 254 nm. Recoveries were better than 95% in the range 25 000–100 000 IU/kg.

Determination of Dexamethasone in Milk by High Pressure Liquid Chromatography

1977 ◽  
Vol 60 (1) ◽  
pp. 210-212 ◽  
Author(s):  
Anneli M DePaolis ◽  
Gabrielle Schnabel ◽  
S E Katz ◽  
Joseph D Rosen
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Holstein Friesian ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Friesian Cows

Abstract A rapid and sensitive high pressure liquid chromatographic method for the analysis of dexamethasone (9α-fluoro-11β,17α,21-trihydroxy-16α-methylpregna-1,4-diene-3,20-dione) in milk has been developed. The corticosteroid can be quantitated at 20 ppb, with the limit of detectability estimated at 5 ppb. Recoveries ranged from 72 to 94%. After dexamethasone was injected into 5 Holstein-Friesian cows, no residues were found in milk, even after the first milking.

High Pressure Liquid Chromatographic Determination of Intermediates and Subsidiary Colors in FD&C Blue No. 2

1980 ◽  
Vol 63 (3) ◽  
pp. 565-571 ◽  
Author(s):  
John E Bailey
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Sulfonic Acid ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract High pressure liquid chromatography (HPLC) is used in the reverse phase mode for the determination of intermediates and subsidiary colors in commercial samples of FD&C Blue No. 2. Isatin-5-sulfonic acid and the lower sulfonated subsidiary color are determined in one run. A second run at lower concentration is required to quantitate the isomeric subsidiary color, since it is present in large amounts and tends to saturate the detector. The calibration and recovery data are treated statistically to evaluate the performance of the method.

USE OF HPLC TO DETERMINE THE EFFECT OF 17β-HYDROXY-7α-METHYLANDROST-5-EN-3-ONE (RMI 12,936) ON PRODUCTION OF PROGESTERONE BY RAT OVARIAN HOMOGENATE

1978 ◽  
Vol 88 (1) ◽  
pp. 157-163 ◽  
Author(s):  
R. B. Taylor ◽  
K. E. Kendle
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Continuous Production ◽  
Hplc Method ◽  
Plasma Progesterone ◽  
Final Level

ABSTRACT A new high pressure liquid chromatography (HPLC) method for the determination of progesterone in the presence of other Δ43-ketosteroids is described. Using this method it is shown that the rate of production of progesterone from pregn-5-ene-3,20-dione by rat ovarian homogenate is initially rapid but falls to zero within 10 min. Experiments indicate that the inhibition is due to the progesterone formed. Inclusion of RMI 12,936 with the pregn-5-ene-3,20-dione substrate results in a lower final level of progesterone and continuous production of 7α-methyltestosterone. The reduction in the levels of progesterone in presence of RMI 12,936 corresponds closely to the reduction in plasma progesterone in vivo following administration of RMI 12,936 described preivously.

High-Pressure Liquid Chromatography of 4,4′-Diazoaminobis-(5-methoxy-2-methylbenzenesulfonic Acid) in FD&C Red No. 40

1976 ◽  
Vol 59 (4) ◽  
pp. 838-845
Author(s):  
Daniel M Marmion
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Chromatographic Method ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Chromatographic Procedure ◽  
The Stability ◽  
Liquid Chromatographic

Abstract Ten analysts representing 7 laboratories participated in the evaluation of a high-pressure liquid chromatographic procedure for the determination of 4,4′-diazoaminobis(5-methoxy-2-methylbenzenesulfonic acid) (DMMA) in FD&C Red No. 40; each collaborator analyzed 7 samples. Between-laboratory standard deviations ranged from 0.0015 to 0.0033% for samples nominally containing 0–0.05% DMMA. The stability of DMMA vs. pH was also studied. It is recommended that the high-pressure liquid chromatographic method be further studied.

High Pressure Liquid Chromatographic Determination of Methyl and Propyl p-Hydroxybenzoates in Comminuted Meats

1981 ◽  
Vol 64 (4) ◽  
pp. 844-847
Author(s):  
Gracia A Perfetti ◽  
Charles R Warner ◽  
Thomas Fazio
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Chromatographic Determination ◽  
Meat Sample ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method was developed for determining methyl and propyl p-hydroxybenzoates (methyl and propyl parabens) in comminuted meats. The parabens were extracted from the meat sample with acetonitrile. After filtering, the extract was analyzed by reverse phase high pressure liquid chromatography, using a 254 nm absorbance detector. Samples of bologna, chicken roll, and chopped ham were fortified with approximately 100,200, and 400 ppm of each paraben. Average recoveries were 92% for methyl paraben and 94% for propyl paraben.

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