Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

Abstract. A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.

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Purification of the Antihemophilic Factor by Gel Filtration on Agarose

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651394 ◽

1969 ◽

Vol 22 (03) ◽

pp. 577-583 ◽

Cited By ~ 3

Author(s):

M.M.P Paulssen ◽

A.C.M.G.B Wouterlood ◽

H.L.M.A Scheffers

Keyword(s):

Factor Viii ◽

Plasma Proteins ◽

Large Scale ◽

Gel Filtration ◽

Large Scale Preparation ◽

Scale Preparation ◽

Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

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Large-scale preparation of the Δ10 form of staphylokinase by in vitro processing of recombinant staphylokinase with purified human plasminogen

Applied Biochemistry and Biotechnology ◽

10.1007/bf02788810 ◽

1998 ◽

Vol 69 (3) ◽

pp. 147-156 ◽

Cited By ~ 4

Author(s):

Debasish Chattopadhyay ◽

Jerry E. Stewart ◽

Lawrence J. DeLucas

Keyword(s):

Large Scale ◽

Large Scale Preparation ◽

In Vitro Processing ◽

Scale Preparation ◽

Human Plasminogen

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Fe-Based Single-Atom Nanozyme with Superior Peroxidase-Mimicking Activity for Enhanced Ultrasensitive Biosensing

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.19533 ◽

2021 ◽

Vol 21 (12) ◽

pp. 6126-6134

Author(s):

Lili Chi ◽

Yuetong Zhang ◽

Yusheng Hua ◽

Qiqi Xu ◽

Mingzhu Lv ◽

...

Keyword(s):

Active Sites ◽

Large Scale ◽

Low Cost ◽

Single Atom ◽

Specificity And Sensitivity ◽

Large Scale Preparation ◽

Species Formation ◽

Biomedical Diagnosis ◽

Scale Preparation

Nanomaterials with intrinsic enzyme-mimicking characteristics, refered to as nanozymes, have become a hot research topic owing to their unique advantages of comparative low cost, high stability and large-scale preparation. Among them, Single-atom nanozymes (SAzymes), as novel nanozymes with abundant atomically dispersed active sites, have caused specific attention in the development of nanozymes for their remarkable catalytic activities, maximum atomic utilization and excellent selectivity, the homogeneous catalytic sites and clear catalytic mechanisms. Herein, a novel single-atom nanozyme based on Fe(III)-doped polydiaminopyridine nanofusiforms (Fe-PDAP SAzyme) was successfully proposed via facile oxidation polymerization strategy. With well-defined coordination structure and abundant Fe-Nx active sites similar to natural metalloproteases, the Fe-PDAP SAzyme exhibits superior peroxidase-like activity by efficiently decomposing H2O2 for hydroxyl radical (.OH) species formation. Based on their superior peroxidase-like activity, colorimetric biosensing of H2O2 and glucose in vitro was performed by using a typical 3,3,5,5-tetramethylbenzidine through a multienzyme biocatalytic cascade platform, exhibiting the superior specificity and sensitivity. This work not only provides a novel promising SAzyme-based biosensor but also paves an avenue for evaluating enzyme activity and broadens the application of other nanozyme-based biosensors in the fields of biomedical diagnosis.

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IMMUNOLOGICAL CROSS REACTION BETWEEN HUMAN CHORIONIC GONADOTROPHIN AND HUMAN PITUITARY GONADOTROPHINS

Acta Endocrinologica ◽

10.1530/acta.0.0530420 ◽

1966 ◽

Vol 53 (3) ◽

pp. 420-428 ◽

Cited By ~ 2

Author(s):

C. Robyn ◽

P. O. Hubinont ◽

E. Diczfalusy

Keyword(s):

Luteinizing Hormone ◽

Follicle Stimulating Hormone ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Human Pituitary ◽

Specific Antisera ◽

Human Menopausal Gonadotrophin ◽

Immunological Cross Reaction

ABSTRACT Immunologically mono-specific antisera prepared against human chorionic gonadotrophin (HCG) preparations completely neutralized in vitro as well as in vivo the luteinizing hormone (LH) and also the follicle-stimulating hormone (FSH) activity of both human hypophyseal gonadotrophin (HHG) and human menopausal gonadotrophin (HMG) preparations.

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Review Lecture: Current status of human conception in vitro

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1985.0010 ◽

1985 ◽

Vol 223 (1233) ◽

pp. 417-448 ◽

Cited By ~ 16

Keyword(s):

Clinical Practice ◽

Frozen Storage ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Current Status ◽

Lh Surge ◽

Infertile Men ◽

Human Menopausal Gonadotrophin ◽

Medical Advances

The scientific and medical advances culminating in the introduction of in vitro fertilization of human oocytes into clinical practice are reviewed. Current methods that use clomiphene, human menopausal gonadotrophin, and both as follicular stimulants, and the endogenous LH surge or an injection of human chorionic gonadotrophin to induce ovulation are described. The effects of multifolliculation, the diurnal rhythm of the LH surge, and the collection of oocytes from the ovary are related to current clinical practice. The success of in vitro fertilization for infertile men and women is considered in relation to the nature of embryonic growth in vitro . Investigations into blastulation, hatching from the zona pellucida and the use of DNA probes for typing embryos are described. The implantation of embryos is the major remaining problem, and physiological and statistical analyses of implantation are given, comparing results from different clinics. The possibility of embryo ‘helping’ and factors leading to multipregnancy are considered, and details are given of the incidence of abortion and the birth of children. The use of immature oocytes, the frozen-storage of embryos and methods of raising the chance of implantation are described briefly.

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