Large-Scale Preparation of Recombinant Ovine Prolactin and Determination of Its in Vitro and in Vivo Activity

2001 ◽  
Vol 22 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Haim Leibovich ◽  
Nina Raver ◽  
Asael Herman ◽  
Ewa L. Gregoraszczuk ◽  
Elisha Gootwine ◽  
...  
Keyword(s):  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Ovine Prolactin

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

Fe-Based Single-Atom Nanozyme with Superior Peroxidase-Mimicking Activity for Enhanced Ultrasensitive Biosensing

2021 ◽  
Vol 21 (12) ◽  
pp. 6126-6134
Author(s):  
Lili Chi ◽  
Yuetong Zhang ◽  
Yusheng Hua ◽  
Qiqi Xu ◽  
Mingzhu Lv ◽  
...  
Keyword(s):  
Active Sites ◽  
Large Scale ◽  
Low Cost ◽  
Single Atom ◽  
Specificity And Sensitivity ◽  
Large Scale Preparation ◽  
Species Formation ◽  
Biomedical Diagnosis ◽  
Scale Preparation

Nanomaterials with intrinsic enzyme-mimicking characteristics, refered to as nanozymes, have become a hot research topic owing to their unique advantages of comparative low cost, high stability and large-scale preparation. Among them, Single-atom nanozymes (SAzymes), as novel nanozymes with abundant atomically dispersed active sites, have caused specific attention in the development of nanozymes for their remarkable catalytic activities, maximum atomic utilization and excellent selectivity, the homogeneous catalytic sites and clear catalytic mechanisms. Herein, a novel single-atom nanozyme based on Fe(III)-doped polydiaminopyridine nanofusiforms (Fe-PDAP SAzyme) was successfully proposed via facile oxidation polymerization strategy. With well-defined coordination structure and abundant Fe-Nx active sites similar to natural metalloproteases, the Fe-PDAP SAzyme exhibits superior peroxidase-like activity by efficiently decomposing H2O2 for hydroxyl radical (.OH) species formation. Based on their superior peroxidase-like activity, colorimetric biosensing of H2O2 and glucose in vitro was performed by using a typical 3,3,5,5-tetramethylbenzidine through a multienzyme biocatalytic cascade platform, exhibiting the superior specificity and sensitivity. This work not only provides a novel promising SAzyme-based biosensor but also paves an avenue for evaluating enzyme activity and broadens the application of other nanozyme-based biosensors in the fields of biomedical diagnosis.

Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

1980 ◽  
Vol 95 (2) ◽  
pp. 232-236 ◽  
Author(s):  
P. G. Crosignani ◽  
P. Donini ◽  
G. C. Lombroso ◽  
S. Donini ◽  
A. Caccamo ◽  
...  
Keyword(s):  
Large Scale ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Beings ◽  
Large Scale Preparation ◽  
Human Menopausal Gonadotrophin ◽  
Scale Preparation ◽  
Human Use ◽  
Sepharose 4B

Abstract. A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.

Microcrystalline Preparation of Akebia Saponin D for its Bioavailability Enhancement in Rats

2015 ◽  
Vol 43 (03) ◽  
pp. 513-528 ◽  
Author(s):  
Qiao-Han Wang ◽  
Xiao-Lin Yang ◽  
Wei Xiao ◽  
Zhen-Zhong Wang ◽  
Gang Ding ◽  
...  
Keyword(s):  
Large Scale ◽  
Oral Absorption ◽  
Bone Fractures ◽  
Aqueous Solubility ◽  
Pharmacokinetic Parameters ◽  
Scanning Calorimetry ◽  
Bioavailability Enhancement ◽  
Large Scale Preparation ◽  
Scale Preparation

Akebia Saponin D (ASD) or asperosaponin VI is the most abundant constituent of the rhizome of Dipsacus asper, which has been used for the treatment of lower back pain, traumatic hematoma and bone fractures. In recent years, it was reported that ASD was a potential treatment strategy for Alzheimer's disease (AD). However, the low bioavailability of ASD limited its clinical utility. Microcrystalline preparation is one of the effective methods to improve drug absorption. The drugs prepared by different methods can present different solid forms (polymorphs), and different polymorphs have significantly different bioavailabilities. The objective of this study was to prepare ASD polymorphs using the different preparation processes and to evaluate their physicochemical properties and oral absorption. ASD-2 obtained by the antisolvent process was simpler and had higher recovery (78.5%) than that of ASD-1 by a two-step macroporous resin column separation (56.5%). The ASD polymorphs were characterized using differential scanning calorimetry (DSC), thermogravimetry analysis (TGA), powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM). The results revealed that ASD-2 existed in microcrystalline form, while ASD-1 was amorphous. Furthermore, the equilibrium solubility, dissolution in aqueous solution and pharmacokinetic parameters of the samples were determined. ASD-2 showed lower aqueous solubility than that of ASD-1 (p < 0.01). In addition, ASD-2 showed lower dissolution with only 65% of the drug released while ASD-1 had a higher dissolution with 99% of drug released at the end of the 180 min testing period. Although ASD-1 significantly increased solubility and dissolution, the AUC 0-20h of ASD-2 was 4.3 times that of the amorphous ASD-1 in vivo. Data suggest that the microcrystalline preparation of ASD-2 is not only reasonable in economy and suitable for large-scale preparation, but also a promising method to enhance bioavailability of ASD.

scholarly journals Development of a static in vitro digestion model for determination of glycemic index

2021 ◽  
Author(s):  
Yesudas Gudivada
Keyword(s):  
Glycemic Index ◽  
Large Scale ◽  
In Vitro Digestion ◽  
Glycemic Response ◽  
In Vivo Testing ◽  
Digestion Kinetics ◽  
Kinetics Of

While in vivo methods have been used to determine the glycemic response of food, they are time consuming, costly, and not suitable for large-scale applications. As an alternative, in vitro digestion models offer fast, reproducible results to study food digestion kinetics that are less expensive than conducting human trials. While there are several in vitro glycemic index (GI) methods used to determine the GI of food, most do not employ methods of in vivo testing. Therefore, we used a static in vitro digestive system, the Dedicated Ryerson University In-vitro Digester (DRUID), that simulates both gastric and intestinal conditions to determine the glycemic response of commonly consumed carbohydrate-containing foods. Samples were collected at regular intervals over a 2h residence time after digestion in the intestinal phase of the DRUID. The DRUID-determined GI values were compared to published in vivo GI values. A Bland-Altman plot showed that there was agreement between the GI values determined from the DRUID compared with published in vivo GI values. In conclusion, the in vitro DRUID can reliably and reproducibly determine the GI across a spectrum of carbohydrate-containing foods, and has the potential to predict the digestion kinetics of novel food products in vivo that may promote human health.

scholarly journals Development of a static in vitro digestion model for determination of glycemic index

2021 ◽  
Author(s):  
Yesudas Gudivada
Keyword(s):  
Glycemic Index ◽  
Large Scale ◽  
In Vitro Digestion ◽  
Glycemic Response ◽  
In Vivo Testing ◽  
Digestion Kinetics ◽  
Kinetics Of

While in vivo methods have been used to determine the glycemic response of food, they are time consuming, costly, and not suitable for large-scale applications. As an alternative, in vitro digestion models offer fast, reproducible results to study food digestion kinetics that are less expensive than conducting human trials. While there are several in vitro glycemic index (GI) methods used to determine the GI of food, most do not employ methods of in vivo testing. Therefore, we used a static in vitro digestive system, the Dedicated Ryerson University In-vitro Digester (DRUID), that simulates both gastric and intestinal conditions to determine the glycemic response of commonly consumed carbohydrate-containing foods. Samples were collected at regular intervals over a 2h residence time after digestion in the intestinal phase of the DRUID. The DRUID-determined GI values were compared to published in vivo GI values. A Bland-Altman plot showed that there was agreement between the GI values determined from the DRUID compared with published in vivo GI values. In conclusion, the in vitro DRUID can reliably and reproducibly determine the GI across a spectrum of carbohydrate-containing foods, and has the potential to predict the digestion kinetics of novel food products in vivo that may promote human health.

Sign in / Sign up

Export Citation Format

Share Document