Large-scale preparation of the Δ10 form of staphylokinase by in vitro processing of recombinant staphylokinase with purified human plasminogen

1998 ◽  
Vol 69 (3) ◽  
pp. 147-156 ◽  
Author(s):  
Debasish Chattopadhyay ◽  
Jerry E. Stewart ◽  
Lawrence J. DeLucas
Keyword(s):  
Large Scale ◽  
Large Scale Preparation ◽  
In Vitro Processing ◽  
Scale Preparation ◽  
Human Plasminogen

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

Fe-Based Single-Atom Nanozyme with Superior Peroxidase-Mimicking Activity for Enhanced Ultrasensitive Biosensing

2021 ◽  
Vol 21 (12) ◽  
pp. 6126-6134
Author(s):  
Lili Chi ◽  
Yuetong Zhang ◽  
Yusheng Hua ◽  
Qiqi Xu ◽  
Mingzhu Lv ◽  
...  
Keyword(s):  
Active Sites ◽  
Large Scale ◽  
Low Cost ◽  
Single Atom ◽  
Specificity And Sensitivity ◽  
Large Scale Preparation ◽  
Species Formation ◽  
Biomedical Diagnosis ◽  
Scale Preparation

Nanomaterials with intrinsic enzyme-mimicking characteristics, refered to as nanozymes, have become a hot research topic owing to their unique advantages of comparative low cost, high stability and large-scale preparation. Among them, Single-atom nanozymes (SAzymes), as novel nanozymes with abundant atomically dispersed active sites, have caused specific attention in the development of nanozymes for their remarkable catalytic activities, maximum atomic utilization and excellent selectivity, the homogeneous catalytic sites and clear catalytic mechanisms. Herein, a novel single-atom nanozyme based on Fe(III)-doped polydiaminopyridine nanofusiforms (Fe-PDAP SAzyme) was successfully proposed via facile oxidation polymerization strategy. With well-defined coordination structure and abundant Fe-Nx active sites similar to natural metalloproteases, the Fe-PDAP SAzyme exhibits superior peroxidase-like activity by efficiently decomposing H2O2 for hydroxyl radical (.OH) species formation. Based on their superior peroxidase-like activity, colorimetric biosensing of H2O2 and glucose in vitro was performed by using a typical 3,3,5,5-tetramethylbenzidine through a multienzyme biocatalytic cascade platform, exhibiting the superior specificity and sensitivity. This work not only provides a novel promising SAzyme-based biosensor but also paves an avenue for evaluating enzyme activity and broadens the application of other nanozyme-based biosensors in the fields of biomedical diagnosis.

Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

1980 ◽  
Vol 95 (2) ◽  
pp. 232-236 ◽  
Author(s):  
P. G. Crosignani ◽  
P. Donini ◽  
G. C. Lombroso ◽  
S. Donini ◽  
A. Caccamo ◽  
...  
Keyword(s):  
Large Scale ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Beings ◽  
Large Scale Preparation ◽  
Human Menopausal Gonadotrophin ◽  
Scale Preparation ◽  
Human Use ◽  
Sepharose 4B

Abstract. A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.

Large-Scale Preparation of Recombinant Ovine Prolactin and Determination of Its in Vitro and in Vivo Activity

2001 ◽  
Vol 22 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Haim Leibovich ◽  
Nina Raver ◽  
Asael Herman ◽  
Ewa L. Gregoraszczuk ◽  
Elisha Gootwine ◽  
...  
Keyword(s):  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Ovine Prolactin

Practical and Efficient Large-Scale Preparation of 4-Methylnicotinic Acid

2016 ◽  
Vol 13 (6) ◽  
pp. 450-452
Author(s):  
Zhaoxue Yu ◽  
Fei Sha ◽  
Peng Wang
Keyword(s):  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

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