scholarly journals Comparative changes in plasma concentrations of progesterone, oestradiol and LH during the ovulatory cycle in a multiple ovulating male line and a single ovulating traditional line of turkeys

Reproduction ◽  
2002 ◽  
Vol 123 (1) ◽  
pp. 127-133
Author(s):  
S Buchanan
Keyword(s):  
Plasma Concentrations ◽  
Ovulatory Cycle ◽  
Male Line

scholarly journals Comparative changes in plasma concentrations of progesterone, oestradiol and LH during the ovulatory cycle in a multiple ovulating male line and a single ovulating traditional line of turkeys

Reproduction ◽  
2002 ◽  
pp. 127-133 ◽  
Author(s):  
S Buchanan ◽  
GW Robertson ◽  
PM Hocking
Keyword(s):  
Peak Plasma ◽  
Ovarian Tissue ◽  
Plasma Concentrations ◽  
High Growth ◽  
Correlated Response ◽  
Ovulatory Cycle ◽  
Plasma Progesterone ◽  
Multiple Ovulation ◽  
Male Line ◽  
The Mean

The aim of this study was to compare the profile of circulating concentrations of LH, progesterone and oestradiol in a multiple ovulating male line with that of a single ovulating line of traditional turkeys. Plasma samples from seven traditional and 12 male-line turkeys were obtained every 3 h for 36 h. Male-line and traditional turkeys had single peaks of LH and progesterone that were of similar duration in both lines. The mean height of the plasma peaks of LH and progesterone were similar in the two lines and there was no detectable peak plasma oestrogen concentration. Mean plasma concentrations of LH and oestrogen were higher in single compared with multiple ovulating turkeys, whereas there were no differences in mean plasma progesterone concentrations. The results indicate that the multiple ovulation state in genetically selected high-growth lines of turkey may be the result of a correlated response in the steroidogenic capacity of ovarian tissue associated with low plasma concentrations of oestrogen rather than of a disturbance in the hormone profile of the ovulatory cycle.

scholarly journals Plasma Concentrations of Immunoreactive (ir)-Inhibin, Gonadotropins and Steroid Hormones during the Ovulatory Cycle of the Duck

2005 ◽  
Vol 51 (3) ◽  
pp. 353-358 ◽  
Author(s):  
PeiXin YANG ◽  
Mohamed S. MEDAN ◽  
Koji Y. ARAI ◽  
Gen WATANABE ◽  
Kazuyoshi TAYA
Keyword(s):  
Steroid Hormones ◽  
Plasma Concentrations ◽  
Ovulatory Cycle

Purified FSH stimulates production of inhibin by the human ovary

1989 ◽  
Vol 122 (1) ◽  
pp. 279-285 ◽  
Author(s):  
H. M. Buckler ◽  
D. L. Healy ◽  
H. G. Burger
Keyword(s):  
Plasma Concentrations ◽  
Follicular Phase ◽  
Human Chorionic Gonadotrophin ◽  
Ovulatory Cycle ◽  
Induction Of Ovulation ◽  
Significant Contamination ◽  
Probable Site ◽  
Ovarian Inhibin ◽  
Urinary Fsh

ABSTRACT Ovarian inhibin production is stimulated by the administration of human menopausal gonadotrophins or following a rise in endogenous LH and FSH. In order to determine whether FSH specifically stimulates inhibin secretion in vivo, immunoassayable serum inhibin levels were measured following the administration of a highly purified preparation of urinary FSH free of significant contamination with LH. Ten anovulatory women underwent a protocol of induction of ovulation with purified FSH and human chorionic gonadotrophin (hCG). During the induction of ovulation, blood samples were taken for radioimmunoassay of FSH, LH, oestradiol, progesterone and inhibin. During the administration of FSH there were increases in plasma concentrations of FSH, oestradiol and inhibin (P < 0·01) but no significant change in the concentration of LH. Oestradiol and inhibin concentrations rose in parallel and were closely correlated (τ = 0·920, n = 110, P < 0·001). There was also a direct correlation between the measured level of FSH and inhibin (τ = 0·512, n = 110, P < 0·05), but there was no correlation between LH and oestradiol, inhibin or FSH. Inhibin (τ− = 0·702, n = 10, P < 0·01) and oestradiol (τ− = 0·691, n = 10, P < 0·01) were correlated with the number of follicles seen on ovarian ultrasound. Levels of oestradiol and inhibin reached a peak on the day of hCG administration or on the following day. Inhibin levels then fell over the next 2 days in all cycles. In an ovulatory cycle resulting in conception, inhibin and oestradiol then rose in parallel with progesterone. We conclude that inhibin appears to be a follicular product which, in the follicular phase of the cycle, is stimulated by FSH alone, with granulosa cells being the probable site of production. Journal of Endocrinology (1989) 122, 279–285

CHANGES IN PLASMA CONCENTRATIONS OF LUTEINIZING HORMONE AFTER INJECTION OF PROGESTERONE AT VARIOUS TIMES DURING THE OVULATORY CYCLE OF THE DOMESTIC HEN (GALLUS DOMESTICUS)

1975 ◽  
Vol 67 (1) ◽  
pp. 59-70 ◽  
Author(s):  
SUSAN C. WILSON ◽  
P. J. SHARP
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Concentrations ◽  
Gallus Domesticus ◽  
Ovulatory Cycle ◽  
Lh Surge ◽  
Incremental Change ◽  
The Mean ◽  
Domestic Hen

SUMMARY Changes in plasma LH concentrations after i.m. injections of 0·5 mg progesterone/kg at various stages of the ovulatory cycle were measured by radioimmunoassay. Four types of response were observed. (1) When the steroid was injected between 4 h after and 12 h before an ovulation, LH levels started to rise after 15–45 min and reached peak values within 90–120 min. The mean maximal incremental change in the level of LH was 1·58 ± 0·10 (s.e.m.) ng/ml (n = 37). (2) In contrast, when progesterone was injected 12–8 h before ovulation, i.e. immediately before a spontaneous pre-ovulatory LH surge, the resulting mean maximal incremental change in LH level, 0·79 ± 0·12 ng/ml (n = 9), was significantly smaller (P < 0·001). (3) If progesterone was injected 8–4 h before ovulation, i.e. when pre-ovulatory LH levels were rising, they immediately started to rise more rapidly and reached peak values within 45 min. The maximal incremental change in the level of LH under these circumstances, 2·34 ± 0·20 ng/ml (n = 12), was significantly greater (P < 0·001 in both cases) than the changes observed in the responses 1 and 2 described above. (4) Levels of LH generally showed no incremental change in response to injections of progesterone given 4–0 h before ovulation, i.e. when pre-ovulatory LH levels were falling. It was concluded that the type of change in plasma LH levels induced by progesterone depended upon the stage of the ovulatory cycle at which the steroid was injected.

scholarly journals Variation in pituitary expression of mRNAs encoding the putative inhibin co-receptor (betaglycan) and type-I and type-II activin receptors during the chicken ovulatory cycle

2005 ◽  
Vol 186 (3) ◽  
pp. 447-455 ◽  
Author(s):  
T M Lovell ◽  
P G Knight ◽  
R T Gladwell
Keyword(s):  
Quantitative Pcr ◽  
Anterior Pituitary ◽  
Transforming Growth Factor ◽  
Plasma Concentrations ◽  
Type I ◽  
Mrna Levels ◽  
Β Subunit ◽  
Ovulatory Cycle ◽  
Α Subunit ◽  
Lh Surge

Secretion of LH and FSH from the anterior pituitary is regulated primarily by hypothalamic GnRH and ovarian steroid hormones. More recent evidence indicates regulatory roles for certain members of the transforming growth factor β (TGFβ) superfamily including inhibin and activin. The aim of this study was to identify expression of mRNAs encoding key receptors and ligands of the inhibin/activin system in the hen pituitary gland and to monitor their expression throughout the 24–25-h ovulatory cycle. Hens maintained on long days (16 h light/8 h dark) were killed 20, 12, 6 and 2 h before predicted ovulation of a midsequence egg (n=8 per group). Anterior pituitary glands were removed, RNA extracted and cDNA synthesized. Plasma concentrations of LH, FSH, progesterone and inhibin A were measured. Real-time quantitative PCR was used to quantify pituitary expression of mRNAs encoding betaglycan, activin receptor (ActR) subtypes (type I, IIA), GnRH receptor (GnRH-R), LH β subunit, FSH β subunit and GAPDH. Levels of mRNA for inhibin/activin βA and βB subunits, inhibin α subunit, follistatin and ActRIIB mRNA in pituitary were undetectable by quantitative PCR (<2 amol/reaction). Significant changes in expression (P<0.05) of ActRIIA and betaglycan mRNA were found, both peaking 6 h before ovulation just prior to the preovulatory LH surge and reaching a nadir 2 h before ovulation, just after the LH surge. There were no significant changes in expression of ActRI mRNA throughout the cycle although values were correlated with mRNA levels for both ActRIIA (r=0.77; P<0.001) and beta-glycan (r=0.45; P<0.01). Expression of GnRH-R mRNA was lowest 20 h before ovulation and highest (P<0.05) 6 h before ovulation; values were weakly correlated with betaglycan (r=0.33; P=0.06) and ActRIIA (r=0.34; P=0.06) mRNA levels. Expression of mRNAs encoding LH β and FSH β subunit were both lowest (P<0.05) after the LH surge, 2 h before ovulation. These results are consistent with an endocrine, but not a local intrapituitary, role of inhibin-related proteins in modulating gonadotroph function during the ovulatory cycle of the hen, potentially through interaction with betaglycan and ActRIIA. In contrast to mammals, intrapituitary expression of inhibin/activin subunits and follistatin appears to be extremely low or absent in the domestic fowl.

Blood levels of ionized calcium, inorganic phosphorus, 1,25-dihydroxycholecalciferol and gonadal hormones in hens laying hard-shelled or shell-less eggs

1986 ◽  
Vol 111 (1) ◽  
pp. 151-157 ◽  
Author(s):  
Y. Nys ◽  
T. M. N'Guyen ◽  
J. Williams ◽  
R. J. Etches
Keyword(s):  
Egg Production ◽  
Plasma Concentrations ◽  
Inorganic Phosphorus ◽  
Gonadal Hormones ◽  
Ionized Calcium ◽  
Control Group ◽  
Blood Levels ◽  
Shell Formation ◽  
Ovulatory Cycle ◽  
Egg Formation

ABSTRACT The production of shell-less eggs was induced in hens to measure the effects of the high demands made by shell formation on the blood minerals and hormones whose concentrations change during egg formation. In control hens laying hard-shelled eggs, the concentration of ionized calcium in plasma decreased at the onset of shell formation, but no change was found in hens laying shell-less eggs. Total calcium concentrations in plasma decreased slightly throughout the ovulation cycle in both groups. Concentrations of inorganic phosphorus in the plasma were increased in the control group during the period of shell formation and decreased when calcification was suppressed. Finally, the concentrations of 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) in plasma were significantly increased 16 and 20 h following an ovulation compared with 4 h after ovulation, or compared with the concentrations observed in hens laying shell-less eggs. The variations in the plasma concentrations of ionized calcium, inorganic phosphorus and 1,25-(OH)2D3 associated with egg formation were therefore absent in hens laying shell-less eggs demonstrating their direct link with shell calcification. On the other hand, suppression of shell production had no influence on the changes in the plasma concentrations of progesterone, oestradiol and testosterone which are associated with the normal ovulatory cycle. It is concluded that the increases in intestinal and uterine calcium transport and in 1,25-(OH)2D3 production which occur at the onset of egg production in hens are mainly controlled by factors involved in maintaining calcium homeostasis rather than by gonadal hormones. J. Endocr. (1986) 111, 151–157

Effects of selection for reduced oviposition interval on plasma concentrations of luteinising hormone during the ovulatory cycle in hens on a 24 h lighting cycle

1985 ◽  
Vol 26 (4) ◽  
pp. 441-451 ◽  
Author(s):  
C. B. Gow ◽  
P. J. Sharp ◽  
N. B. Carter ◽  
R. J. Scaramuzzi ◽  
B. L. Sheldon ◽  
...  
Keyword(s):  
Luteinising Hormone ◽  
Plasma Concentrations ◽  
Ovulatory Cycle ◽  
Selection For
Sign in / Sign up

Export Citation Format

Share Document