Fast-Tracking Isolation, Identification and Characterization of New Microalgae for Nutraceutical and Feed Applications

Microalgae contain high-value biochemical compounds including fatty acids (FA), protein and carotenoids, and are promising bioresources to enhance nutrition of food and animal feed. Important requirements for commercial strains are rapid growth and high productivities of desirable compounds. As these traits are believed to be found in aquatic environments with fluctuating conditions, we collected microalgae from marine and freshwater environments that are subjected to eutrophication and/or tidal fluctuations. Using this directed approach, 40 monoalgal cultures were isolated and 25 identified through 18S rDNA sequencing and morphological characterization. Based on their high growth rates (0.28–0.60 day−1) and biomass productivities (0.25–0.44 g L−1day−1) in commercial fertilizer under standardized conditions, six new strains were selected. Scenedesmus sp. GW63 produced quality FA-rich biomass with high omega-3 polyunsaturated FA (28.5% of total FA (TFA)) contents, especially α-linolenic acid (ALA; 20.0% of TFA) with a very low n-6/n-3 ratio (0.4), and high FA productivity (32.6 mg L−1 day−1). A high protein productivity (34.5 mg L−1 day−1) made Desmodesmus sp. UQL1_26 (33.4% of dry weight (DW)) attractive as potential protein-rich feed and nutrition supplement. Monoraphidium convolutum GW5 displayed valuable carotenoid production (0.8% DW) with high carotenoid accumulation capability (0.8 mg L−1 day−1). This research provides a pathway for fast-tracking the selection of high-performing local microalgae from different environments for nutraceuticals, functional foods and animal feed applications.

Application of Heterogeneous Network Oriented to NoSQL Database in Optimal Postevaluation Indexes of Construction Projects

With the advent of the Internet Web 2.0 era, storage devices used to store website data are developing at an ever-increasing high-growth rate and a diversified trend. The focus on the structured data storage model has reduced the responsiveness of traditional relational databases to data changes. NoSQL database is scalable, has a powerful and flexible data model and a large amount of data, and has an increasing application potential in the memory field. Heterogeneous networks are composed of third-party computers, network equipment, and systems. Network types are usually used for other protocols to support other functions and applications. The research on heterogeneous networks can be traced back to the BARWAN project that started in 1995 at the University of California, Berkeley. The project leader RHKatz merged multiple types of nested networks for the first time to form heterogeneous network requirements for various future terminal services. Construction engineering refers to an engineering entity formed by installing pipelines and equipment that support the construction of various houses and ancillary facilities. “House construction” refers to projects with roofs, beams, columns, walls, and foundations that can form internal spaces to meet people’s needs in production, living, learning, and public activities. Among them, the engineering evaluation index is a statistical index used to evaluate and compare the quality and effects of social and economic activities through the use of equipment, such as capital turnover rate and employee labor efficiency. It is the exchange of corporate performance evaluation content and the expression of corporate performance evaluation content.

Dietary supplements based on modified and enriched with amino acids zeolite in the cultivation of young turkeys

Relevance. Modern turkey crosses have a genetically determined high growth rate. These farm birds are very sensitive to even minor fluctuations in their diet, are susceptible to a deficiency of amino acids, protein, mineral elements and vitamins, which can contribute to a decrease in their productivity, the development of pathological processes and diseases. The use of modern technologies and scientific achievements has made it possible to prepare a new product — modified zeolite. This natural mineral is already free of impurities and microbes, with open pore windows, has a high ability to selectively exchange, and is obtained by various methods of activating zeolite in industrial conditions. At the same time, Ulyanovsk specialists have mastered the technology of enriching modified zeolite with useful substances.Methods. The aim of the work was to study the effect on the body of turkeys and the level of their productivity of additives based on modified zeolite enriched with amino acids. The experiment was organized for 60 days on 250 turkeys of the breed Hybrid Cread-mayker aged 55–60 days in the conditions of the Ulyanovsk region. The control group received the main ration (MR), the experimental group was given once a day in addition to the MR in the morning feeding in a mixture with mixed feed 5% additives of modified zeolite enriched with amino acids.Results. The inclusion of a supplement based on modified zeolite enriched with amino acids in the diet of turkeys does not have a negative effect on the hematological parameters of their blood, all indicators corresponded to the physiological norm. During the experiment in the 2nd group, the live weight of poultry was obtained larger by 8.70%, which amounted to 12,296 kg, respectively, more profit was obtained from the sale of turkey meat, total revenue was 133,210, and additional profit was 27,960 rubles, 2.41 rubles of profit was received for 1 ruble of costs. Тhe data indicate the normalization of mineral homeostasis in the body of turkeys and better absorption of nutrients in the feed diet.

One Year Monitoring of Ecological Interaction of Silurus glanis in a Novel Invaded Oligotrophic Deep Lake (Lake Maggiore)

The European catfish (Silurus glanis) was introduced in Italy during the last century for aquaculture purposes, and now it is well-established. S. glanis is an invasive species and a top predator that can deplete prey supply in the surrounding habitat, leading to changes in the aquatic food web. Consequently, its presence is considered a threat to native fish populations. Its presence in the Lake Maggiore (Northern Italy) is recent and there is a lack of knowledge about its ability to completely exploit this new ecosystem throughout the year. This study corroborated the ability of European catfish to exploit both pelagic and littoral habitats, promoting trophic interactions in both habitats. Over 2019, multiple sampling approaches have been applied by collecting S. glanis and analysing its stomach contents with the aim of inferring interactions with the freshwater community. Its diet was mainly based on crayfish (Orconectes limosus), followed by six prey fish and the genus Corbicula; two fish species (Padogobius bonelli and Salaria fluviatilis) were added to the list of known prey fish. Notably and alarmingly for the early potential top-down pressure towards all trophic levels, young individuals were proved to also feed on fish and crayfish. S. glanis showed the ability to hunt at deep depths (>60 m) and a high growth rate, despite Lake Maggiore being oligotrophic.

Examining the cohesiveness and nestedness entrepreneurial ecosystems: evidence from British FinTechs

It is not clear if entrepreneurial ecosystems are cohesive wholes within a region that support high-growth entrepreneurship across a variety of sectors or if ecosystems are made up of several, nested sectoral-specific sub-ecosystems. This debate speaks to larger disagreements about what entrepreneurial ecosystems are and how they work. This paper addresses this research gap by using a novel methodology based on career history data of founders and top management teams of high-growth FinTech ventures. This method is used to classify the backgrounds of 1,570 individuals in 380 British FinTech firms based on their prior job histories and employers into categories such as technology or finance. The paper finds substantial evidence of nestedness in the ecosystems, but rather than FinTech ecosystems being specialized finance or technology clusters, more generic forms of managerial know-how remain crucial to firm innovation and growth. This suggests that even very advanced ecosystems remain nested, with few cross-over points between different communities.

Hairy Roots

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

Management of Quality, Satisfaction, and Loyalty of Rural Destinations

Tourism is a major global industry that, in its heyday, has become significantly concerned with quality and related themes, satisfaction and loyalty. This chapter aims to identify the factors that influence the quality of a rural destination and the associated visitor satisfaction and loyalty. These factors in turn have an impact on destination management, but also other stakeholders such as tourism entrepreneurs in the destination or residents. In 2020, tourism has undergone significant changes due to the pandemic caused by the SARS-CoV-2 virus. For instance, travel abroad was regulated through government regulations, which caused an increase in demand for rural tourism. As the author's research has shown, some quality factors have undergone significant changes. Even the quality management to achieve visitor satisfaction and loyalty has proven to be very important, especially in the sense that by gaining loyalty at this specific time, it can ensure that visitor numbers are maintained even after the pandemic period when the return of and high growth in overseas travel is expected.