A and C Genome Distinction and Chromosome Identification in Brassica napus by Sequential Fluorescence in Situ Hybridization and Genomic in Situ Hybridization

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.Key words: Triticum aestivum, chromosome identification, fluorescence in situ hybridization, repetitive DNA sequences.

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Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae)

Genome ◽

10.1139/g00-109 ◽

2001 ◽

Vol 44 (2) ◽

pp. 275-283 ◽

Cited By ~ 13

Author(s):

Marian Ørgaard ◽

Kesara Anamthawat-Jónsson

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Genomic Dna ◽

Genomic In Situ Hybridization ◽

Pseudoroegneria Spicata ◽

Genome Constitution ◽

Elytrigia Repens ◽

Elymus Caninus ◽

Elymus Species

The genome constitution of Icelandic Elymus caninus, E. alaskanus, and Elytrigia repens was examined by fluorescence in situ hybridization using genomic DNA and selected cloned sequences as probes. Genomic in situ hybridization (GISH) of Hordeum brachyantherum ssp. californicum (diploid, H genome) probe confirmed the presence of an H genome in the two tetraploid Elymus species and identified its presence in the hexaploid Elytrigia repens. The H chromosomes were painted uniformly except for some chromosomes of Elytrigia repens which showed extended unlabelled pericentromeric and subterminal regions. A mixture of genomic DNA from H. marinum ssp. marinum (diploid,Xa genome) and H. murinum ssp. leporinum (tetraploid,Xu genome) did not hybridize to chromosomes of the Elymus species or Elytrigia repens, confirming that these genomes were different from the H genome. The St genomic probe from Pseudoroegneria spicata (diploid) did not discriminate between the genomes of the Elymus species, whereas it produced dispersed and spotty hybridization signals most likely on the two St genomes of Elytrigia repens. Chromosomes of the two genera Elymus and Elytrigia showed different patterns of hybridization with clones pTa71 and pAes41, while clones pTa1 and pSc119.2 hybridized only to Elytrigia chromosomes. Based on FISH with these genomic and cloned probes, the two Elymus species are genomically similar, but they are evidently different from Elytrigia repens. Therefore the genomes of Icelandic Elymus caninus and E. alaskanus remain as StH, whereas the genomes of Elytrigia repens are proposed as XXH.Key words: Elymus, Elytrigia, H genome, St genome, in situ hybridization.

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Characterization of somatic hybrids between Brassica napus and Eruca sativa using species-specific repetitive sequences and genomic in situ hybridization

Plant Science ◽

10.1016/s0168-9452(96)04575-x ◽

1997 ◽

Vol 123 (1-2) ◽

pp. 133-142 ◽

Cited By ~ 33

Author(s):

Jan Fahleson ◽

Ulf Lagercrantz ◽

Armand Mouras ◽

Kristina Glimelius

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Somatic Hybrids ◽

Repetitive Sequences ◽

Genomic In Situ Hybridization ◽

Eruca Sativa ◽

Species Specific

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The genomic identification of some monosomics of Avena sativa L. cv. Sun II using genomic in situ hybridization

Genome ◽

10.1139/g95-094 ◽

1995 ◽

Vol 38 (4) ◽

pp. 747-751 ◽

Cited By ~ 30

Author(s):

J. M. Leggett ◽

G. S. Markhand

Keyword(s):

In Situ Hybridization ◽

Avena Sativa ◽

Genomic Dna ◽

Diploid Species ◽

Genomic In Situ Hybridization ◽

Chromosome Translocations ◽

C Genome

Genomic in situ hybridization using total genomic DNA extracted from the C genome diploid species Avena eriantha (2n = 2x = 14, genome CpCp) was used to identify monosomics (2n = 6x − 1 = 41) of the constituent genomes of the hexaploid cultivated oat A. sativa L. cv. Sun II (2n = 6x = 42, genomes AACCDD). The results demonstrate 3 AD/C and 6 C/AD chromosome translocations, indicate that five of the missing monosomics are derived from the C genome, and show that there are duplicates within the partial monosomic series. Chromosome polymorphisms between some monosomic lines are also demonstrated.Key words: Avena, monosomics, genomic in situ hybridization, genomic identification.

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Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization

Genome ◽

10.1139/gen-41-5-691 ◽

1998 ◽

Vol 41 (5) ◽

pp. 691-701 ◽

Cited By ~ 12

Author(s):

Marina Skarzhinskaya ◽

Jan Fahleson ◽

Kristina Glimelius ◽

Armand Mouras

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Genome Organization ◽

Somatic Hybrids ◽

Genomic In Situ Hybridization ◽

Lesquerella Fendleri

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Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploid oat species (genus Avena)

Genome ◽

10.1139/g94-087 ◽

1994 ◽

Vol 37 (4) ◽

pp. 613-618 ◽

Cited By ~ 98

Author(s):

E. N. Jellen ◽

B. S. Gill ◽

T. S. Cox

Keyword(s):

In Situ Hybridization ◽

Avena Sativa ◽

Genomic In Situ Hybridization ◽

Hybridization Pattern ◽

C Banding ◽

Intergenomic Translocations ◽

C Genome ◽

Total Dna

The genomic in situ hybridization (GISH) technique was used to discriminate between chromosomes of the C genome and those of the A and A/D genomes in allopolyploid oat species (genus Avena). Total biotinylated DNA from A. strigosa (2n = 2x = 14, AsAs genome) was mixed with sheared, unlabelled total DNA from A. eriantha (2n = 2x = 14, CpCp) at a ratio of 1:200 (labelled to unlabelled). The resulting hybridization pattern consisted of 28 mostly labelled and 14 mostly unlabelled chromosomes in the hexaploids. Attempts to discriminate between chromosomes of the A and D genomes in A. sativa (2n = 6x = 42, AACCDD) were unsuccessful using GISH. At least eight intergenomic translocation segments were detected in A. sativa 'Ogle', several of which were not observed in A. byzantina 'Kanota' (2n = 6x = 42, AACCDD) or in A. sterilis CW 439-2 (2n = 6x = 42, AACCDD). At least five intergenomic translocation segments were observed in A. maroccana CI 8330 'Magna' (2n = 4x = 28, AACC). In both 'Ogle' and 'Magna', positions of most of these translocations matched with C-banding patterns.Key words: Avena sativa, oat, in situ hybridization, C-banding, Avena macrostachya.

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Intra- and intergenomic chromosome pairings revealed by dual-color GISH in trigenomic hybrids of Brassica juncea and B. carinata with B. maurorum

Genome ◽

10.1139/g09-082 ◽

2010 ◽

Vol 53 (1) ◽

pp. 14-22 ◽

Cited By ~ 6

Author(s):

X. C. Yao ◽

X. Z. Du ◽

X. H. Ge ◽

J. P. Chen ◽

Z. Y. Li

Keyword(s):

In Situ Hybridization ◽

Genome Evolution ◽

Chromosome Pairing ◽

Genomic In Situ Hybridization ◽

Crop Breeding ◽

Obvious Effect ◽

Dual Color ◽

C Genome ◽

Relationship Of

From dual-color genomic in situ hybridization (GISH) analysis of three trigenomic hybrids, Brassica maurorum (MM, 2n = 16) × B. juncea (AABB, 2n = 36) (M.AB), B. maurorum × B. carinata (BBCC, 2n = 34) (M.BC), and B. carinata × B. maurorum (BC.M), the three genomes of each hybrid were distinguished and autosyndesis and allosyndesis were evaluated. In M.AB, up to two autosyndetic bivalents occurred among the chromosomes of each genome; a maximum of three allosyndetic bivalents appeared between A-B, A-M, and B-M genomes. The similar pairings in M.BC and BC.M suggested that the cytoplasm of B. maurorum or B. carinata had no obvious effect on chromosome pairing. In M.BC and BC.M, a maximum of one autosyndetic bivalent was found for B and M genomes, but two were found for the C genome; from 0 to 2 allosyndetic bivalents were observed between B-C, B-M, and C-M genomes. The B-M allosyndesis frequency was higher than the A-M or C-M allosyndesis frequency in these hybrids, revealing the closer relationship of B and M genomes. The allosyndesis frequency was higher than the autosyndesis frequency among A, B, and C genomes in these combinations, suggesting that intergenomic homoeology was higher than intragenomic homoeology. The implications for genome evolution and crop breeding are discussed.

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