Differentiation of closely related genomes and chromosome identification in Brassica napus L. By simultaneous fluorescence in situ hybridization and genomic in situ hybridization

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.Key words: Triticum aestivum, chromosome identification, fluorescence in situ hybridization, repetitive DNA sequences.

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Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae)

Genome ◽

10.1139/g00-109 ◽

2001 ◽

Vol 44 (2) ◽

pp. 275-283 ◽

Cited By ~ 13

Author(s):

Marian Ørgaard ◽

Kesara Anamthawat-Jónsson

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Genomic Dna ◽

Genomic In Situ Hybridization ◽

Pseudoroegneria Spicata ◽

Genome Constitution ◽

Elytrigia Repens ◽

Elymus Caninus ◽

Elymus Species

The genome constitution of Icelandic Elymus caninus, E. alaskanus, and Elytrigia repens was examined by fluorescence in situ hybridization using genomic DNA and selected cloned sequences as probes. Genomic in situ hybridization (GISH) of Hordeum brachyantherum ssp. californicum (diploid, H genome) probe confirmed the presence of an H genome in the two tetraploid Elymus species and identified its presence in the hexaploid Elytrigia repens. The H chromosomes were painted uniformly except for some chromosomes of Elytrigia repens which showed extended unlabelled pericentromeric and subterminal regions. A mixture of genomic DNA from H. marinum ssp. marinum (diploid,Xa genome) and H. murinum ssp. leporinum (tetraploid,Xu genome) did not hybridize to chromosomes of the Elymus species or Elytrigia repens, confirming that these genomes were different from the H genome. The St genomic probe from Pseudoroegneria spicata (diploid) did not discriminate between the genomes of the Elymus species, whereas it produced dispersed and spotty hybridization signals most likely on the two St genomes of Elytrigia repens. Chromosomes of the two genera Elymus and Elytrigia showed different patterns of hybridization with clones pTa71 and pAes41, while clones pTa1 and pSc119.2 hybridized only to Elytrigia chromosomes. Based on FISH with these genomic and cloned probes, the two Elymus species are genomically similar, but they are evidently different from Elytrigia repens. Therefore the genomes of Icelandic Elymus caninus and E. alaskanus remain as StH, whereas the genomes of Elytrigia repens are proposed as XXH.Key words: Elymus, Elytrigia, H genome, St genome, in situ hybridization.

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Characterization of somatic hybrids between Brassica napus and Eruca sativa using species-specific repetitive sequences and genomic in situ hybridization

Plant Science ◽

10.1016/s0168-9452(96)04575-x ◽

1997 ◽

Vol 123 (1-2) ◽

pp. 133-142 ◽

Cited By ~ 33

Author(s):

Jan Fahleson ◽

Ulf Lagercrantz ◽

Armand Mouras ◽

Kristina Glimelius

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Somatic Hybrids ◽

Repetitive Sequences ◽

Genomic In Situ Hybridization ◽

Eruca Sativa ◽

Species Specific

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Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization

Genome ◽

10.1139/gen-41-5-691 ◽

1998 ◽

Vol 41 (5) ◽

pp. 691-701 ◽

Cited By ~ 12

Author(s):

Marina Skarzhinskaya ◽

Jan Fahleson ◽

Kristina Glimelius ◽

Armand Mouras

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Genome Organization ◽

Somatic Hybrids ◽

Genomic In Situ Hybridization ◽

Lesquerella Fendleri

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Behaviour of Sinapis alba chromosomes in a Brassica napus background revealed by genomic in-situ hybridization

Chromosome Research ◽

10.1007/s10577-005-1017-2 ◽

2005 ◽

Vol 13 (8) ◽

pp. 819-826 ◽

Cited By ~ 31

Author(s):

Y. P. Wang ◽

X. X. Zhao ◽

K. Sonntag ◽

P. Wehling ◽

R. J. Snowdon

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Sinapis Alba ◽

Genomic In Situ Hybridization

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Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum–Secale–Thinopyrum trigeneric hybrids

Genome ◽

10.1139/gen-2017-0025 ◽

2017 ◽

Vol 60 (8) ◽

pp. 687-694 ◽

Cited By ~ 3

Author(s):

Yi Dai ◽

Yamei Duan ◽

Dawn Chi ◽

Huiping Liu ◽

Shuai Huang ◽

...

Keyword(s):

Molecular Markers ◽

In Situ Hybridization ◽

Genomic In Situ Hybridization ◽

Chromosome Identification ◽

Distant Hybridization ◽

Wheat Improvement ◽

Thinopyrum Elongatum ◽

Trigeneric Hybrids

It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R–E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.

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Transgenic Brassica napus fields and Brassica rapa weeds in Quebec: sympatry and weed-crop in situ hybridization

Canadian Journal of Botany ◽

10.1139/b06-135 ◽

2006 ◽

Vol 84 (12) ◽

pp. 1842-1851 ◽

Cited By ~ 36

Author(s):

Marie-Josée Simard ◽

Anne Légère ◽

Suzanne I. Warwick

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Brassica Rapa ◽

Transgenic Crop ◽

Herbarium Specimens ◽

Field Margins ◽

Brassica Napus L ◽

Sampling Locations ◽

Range Overlap

Hybridization between the herbicide-resistant transgenic crop Brassica napus L. (canola) and its weedy relative Brassica rapa L. (bird rape) has been documented in Quebec. Our goal was to evaluate the actual hybridization potential based on range overlap and actual in situ hybridization rates. This was done by mapping B. napus canola fields, comparing them with the sampling locations of B. rapa herbarium specimens from Quebec, gathering information on the presence of B. rapa in certified canola seed production fields, and surveying for B. rapa populations located in, or close to B. napus field margins. Progeny from these populations were screened for herbicide resistance (HR) and for the presence of the HR transgene. Two fields were also selected to evaluate B. rapa density effects on hybridization rates. Significant sympatry was observed in several areas of the province; hybridization occurred in all eight populations (1.1% to 17.5% hybrid seed) located in field margins and in one (1.1%) out of three populations located less than 10 m from a B. napus field. Hybridization rates decreased exponentially as B. rapa density increased, but interplant rates (0% to 68%) were highly variable. Environmental problems could be generated by the release of B. napus crops with traits conferring fitness benefits in nonmanaged areas.

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Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum

Genome ◽

10.1139/g03-020 ◽

2003 ◽

Vol 46 (3) ◽

pp. 469-472 ◽

Cited By ~ 20

Author(s):

A Benabdelmouna ◽

G Guéritaine ◽

M Abirached-Darmency ◽

H Darmency

Keyword(s):

In Situ Hybridization ◽

Brassica Napus ◽

Oilseed Rape ◽

Interspecific Hybrid ◽

Interspecific Hybrids ◽

Genomic In Situ Hybridization ◽

Interspecific Crosses ◽

Wild Radish ◽

Raphanus Raphanistrum

Genomic in situ hybridization (GISH) applied to the F1 interspecific hybrid between oilseed rape (Brassica napus, AACC, 2n = 38) and wild radish (Raphanus raphanistrum, RrRr, 2n = 18) showed the predicted 19 chromosomes from B. napus and 9 chromosomes from R. raphanistrum. The very low female fertility of these interspecific hybrids when backcrossed to R. raphanistrum led to only two descendants. Their chromosome number varied between 45 and 48. Both of these progenies showed only 9 chromosomes from R. raphanistrum and 3639 chromosomes from B. napus. These results indicate the efficiency and limits of GISH as a suitable tool to assess and interpret the behavior of chromosomes after such interspecific crosses. The unexpected chromosome combination is discussed.Key words: genomic in situ hybridization, interspecific hybrid, introgression, oilseed rape, wild radish.

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