scholarly journals Variability of P26 and P10 genes in Colombian isolates of Potato yellow vein virus (PYVV)

2019 ◽  
Vol 37 (2) ◽  
pp. 129-143
Author(s):  
Jhon Calderón ◽  
Teresa Mosquera Vásquez ◽  
Ángela María Vargas
Keyword(s):  
Negative Selection ◽  
Pcr Amplification ◽  
Potato Production ◽  
Diversity Indices ◽  
Trialeurodes Vaporariorum ◽  
Yellow Vein ◽  
Rt Pcr ◽  
Pathogenic Factors ◽  
Vein Disease ◽  
Potato Yellow Vein Virus

Potato yellow vein virus (PYVV) is the causal agent of the potato yellow vein disease and can reduce potato production up to 50%. This virus also infects tomatoes and can remain asymptomatic in plants. PYVV transmission is mediated by vegetative seed, the vector Trialeurodes vaporariorum, and grafts. Its genome has the P26 and P10 genes that are orthologues in the Crinivirus genus, which have been characterized as pathogenic factors and have not been studied in PYVV. We analyzed the variability of P26 and P10 from 45 and 48 sequences, which were obtained by RT-PCR amplification of the total RNA of symptomatic potato leaves from the provinces of Nariño, Cundinamarca, and Boyaca (Colombia). We included sequences of each gene of the PYVV genome of potato and tomato isolates from GenBank. The variability in these genes is influenced by the flow and uncontrolled use of vegetative seed between different provinces, that favor the dispersion of viral variants. In addition, the variability analysis based on maximum likelihood trees, haplotypes, and diversity indices showed that P26 is more variable than P10 and both are more variable in Andigena than in Phureja potatoes. The Tajima and Fu and Li tests revealedthat these genes are subject to negative selection.

Economic injury levels for the potato yellow vein disease and its vector, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), affecting potato crops in the Andes

2019 ◽  
Vol 119 ◽  
pp. 52-58 ◽  
Author(s):  
Diego F. Rincon ◽  
Diego F. Vasquez ◽  
Hugo Fernando Rivera-Trujillo ◽  
Carlos Beltrán ◽  
Felipe Borrero-Echeverry
Keyword(s):  
Trialeurodes Vaporariorum ◽  
Yellow Vein ◽  
The Andes ◽  
Economic Injury ◽  
Potato Crops ◽  
Vein Disease

scholarly journals Potato Virus Y (PVY) y Potato Yellow Vein Virus (PYVV) en Infecciones Mixtas No Causan Síntomas Atípicos en Plantas de Papa

2016 ◽  
Vol 11 (2) ◽  
pp. 26 ◽  
Author(s):  
Jenny Paola Alfaro García ◽  
Liliana Franco-Lara
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Yellow Vein ◽  
Solanum Phureja ◽  
Rt Pcr ◽  
Potato Yellow Vein Virus

Observaciones anteriores en cultivos de papa (<em>Solanum tuberosum</em> y <em>Solanum phureja</em>) mostraron síntomas llamados atípicos en este trabajo, que consistían en hojas con manchas irregulares verde oscuro sobre un fondo amarillo intenso, que sugieren la presencia de virus. Estos síntomas no correlacionan con ningún virus descrito para papa en Colombia. En plantas, existen reportes de infecciones virales con dos o más virus que llevan a interacciones como sinergismo o antagonismo. En este trabajo se evaluó la hipótesis de que los síntomas atípicos podrían ser resultado de infecciones mixtas entre PYVV (<em>Potato yellow vein virus) (Crinivirus) </em>y PVY (<em>Potato virus Y</em>) (<em>Potyirus)</em>, pues ambos virus son comunes en cultivos de papa en Colombia. Se reportan resultados de RT-PCR (reacción en cadena de la polimerasa mediada por retrotranscripción) para la detección de PYVV y PVY, y de ELISA para PVX, PVS, PVM y PRLV en 57 plantas con y sin síntomas atípicos provenientes de campo. Los resultados no apoyan la hipótesis planteada, pues de 10 plantas con síntomas atípicos evaluadas solo una estaba infectada con los dos virus.  Por otro lado, coinfecciones de PVY y PYVV se observaron en plantas sin síntomas aparentes (4 plantas de 5 evaluadas) y en plantas con síntomas característicos de PYVV (17 plantas de 37 evaluadas). Del total de 20 plantas evaluadas por ELISA, 18 presentaban infección por PVX aunque no se observaron síntomas asociados a este virus. Ocho de estas plantas, además de PVX estaban infectadas también con PVY y PYVV, pero mostraban síntomas de característicos de PYVV, lo que sugiere que PVX tampoco correlaciona con los síntomas atípicos. Se sugiere la presencia de un virus no reportado infectando el cultivo de papa en Colombia.

scholarly journals Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia

2014 ◽  
Vol 32 (2) ◽  
pp. 213-223 ◽  
Author(s):  
Angela Villamil-Garzón ◽  
Wilmer J. Cuellar ◽  
Mónica Guzmán-Barney
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Mixed Infection ◽  
Disease Diagnosis ◽  
Yellow Vein ◽  
Breeding Programs ◽  
Potato Plants ◽  
Next Generation Sequencing Ngs ◽  
Vein Disease ◽  
Potato Yellow Vein Virus

The Potato yellow vein virus (PYVV), a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P) and four Group Andigena (A), were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS). The Potato virus Y (PVY) complete genome (similar to N strain) and the Potato virus V (PVV) partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and/or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.

scholarly journals Secuenciación del genoma completo del Potato yellow vein virus (PYVV) en tomate (Solanum lycopersicum) en Colombia

2017 ◽  
Vol 22 (1) ◽  
pp. 5 ◽  
Author(s):  
Laura Muñoz Baena ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Marín Montoya
Keyword(s):  
Solanum Lycopersicum ◽  
Yellow Vein ◽  
Rt Pcr ◽  
Potato Yellow Vein Virus

Potato yellow vein virus (PYVV), es uno de los fitopatógenos más limitantes para la producción de papa en la región de Los Andes. A pesar que se le ha detectado infectando tomate en Colombia, el conocimiento de las características biológicas de las cepas presentes en este hospedante es muy limitado. En este estudio, utilizando secuenciación masiva de nueva generación (NGS), se obtuvo la secuencia completa de los tres segmentos genómicos del PYVV en plantas de tomate en Marinilla (Antioquia) y se evalúo la utilidad de tres juegos de cebadores para su detección mediante pruebas de RT-PCR convencional y en tiempo real (RT-qPCR). El genoma de la secuencia consenso presentó tamaños de 8043 nt (ARN1), 5346 nt (ARN2) y 3896 nt (ARN3) y se identificaron los diez ORF previamente reportados en este virus, aunque, en general, éstos presentaron menores| niveles de identidad que los registrados entre cepas de PYVV de papa. Análisis de variación y de selección identificaron dos regiones en los ORF MET/HEL y CPm que presentan selección positiva, lo que podría estar asociado a la adaptación por hospedante. Los tres juegos de cebadores amplificaron las regiones esperadas de la cápside de PYVV, siendo posible identificar, por diferencias en valores de temperatura de fusión (Tm) y por secuenciación Sanger, la ocurrencia de al menos dos variantes principales de este virus en el Oriente Antioqueño, lo que concuerda con los niveles moderados de polimorfismos encontrados en las secuencias obtenidas por NGS.

scholarly journals First Report of Beet pseudo-yellows virus and Strawberry pallidosis associated virus in Strawberry in Peru

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1457-1457 ◽  
Author(s):  
W. M. Wintermantel ◽  
S. Fuentes ◽  
C. Chuquillanqui ◽  
L. F. Salazar
Keyword(s):  
Coat Protein ◽  
Pcr Amplification ◽  
Trialeurodes Vaporariorum ◽  
Rt Pcr ◽  
Greenhouse Whitefly ◽  
First Report ◽  
Wide Range ◽  
Pcr Products ◽  
Sequencing And Expression ◽  
Beet Pseudo Yellows Virus

During a 2006 survey for the presence of criniviruses in Peru, large numbers of greenhouse whitefly (Trialeurodes vaporariorum) were observed infesting strawberry (Fragaria × ananassa) fields near Huaral on the central coast of Peru. Plants exhibited a wide range of symptoms including stunting and reddening of leaves. These symptoms are characteristic of those induced by the presence of the criniviruses Beet pseudo-yellows virus (BPYV) and/or Strawberry pallidosis associated virus (SPaV) together with any of a number of different strawberry-infecting viruses (1,3). The virus complex causes older leaves to develop a red color, vein and petiole reddening, roots become stunted, and plants fail to develop. Leaf samples with varying symptoms were collected from 22 plants from 2 fields, each planted with a different cultivar. Total nucleic acid was extracted, spotted onto positively charged nylon membranes, and tested by hybridization with probes specific to the minor coat protein (CPm) gene of BPYV (2) and coat protein (CP) gene of SPaV (4). Results identified the presence of BPYV, SPaV, or both viruses in mixed infections in symptomatic strawberry, while control plants were infected with each virus individually. No signal was detected in virus-free strawberry. Secondary confirmation was obtained using probes specific to the RNA-dependent RNA polymerase (RdRp) genes of SPaV and BPYV. The SPaV probe corresponded to nucleotides 6116–6599 of SPaV RNA1 (GenBank Accession No. NC_005895), whereas the BPYV probe corresponded to nucleotides 6076–6447 of BPYV RNA1 (GenBank Accession No. NC_005209). All probes were generated by reverse-transcription polymerase chain reaction (RT-PCR) amplification using sequence-specific primers, cloning of RT-PCR products into pGEM-T Easy (Promega, Madison, WI), confirmation by sequencing, and expression as digoxygenin-labeled transcript probes (Roche, Indianapolis, IN). Field 1, containing cv. Fern Sancho, had the largest number of symptomatic and infected plants (5 of 12 BPYV, 6 of 12 SPaV, and 4 of 12 with both). Only 1 of 10 plants from field 2 containing cv. Tajo Holandesa was infected, but with both SPaV and BPYV. BPYV and SPaV are transmitted by the greenhouse whitefly (T. vaporariorum), although BPYV is transmitted much more efficiently and has a broader host range than SPaV (4). Movement of these viruses in Peru is likely a result of both propagation by runners and vector transmission. To our knowledge, this is the first report of either virus in Peru. References: R. R Martin and I. E. Tzanetakis. Plant Dis. 90:384, 2006. (2) I. E. Tzanetakis and R. R. Martin. Plant Dis. 88:223, 2004. (3) I. E. Tzanetakis et al. Plant Dis. 87:1398, 2003. (4) I. E. Tzanetakis et al. Plant Dis. 90:1343, 2006.

scholarly journals Analysis of the RNA of Potato yellow vein virus: evidence for a tripartite genome and conserved 3′-terminal structures among members of the genus Crinivirus

2004 ◽  
Vol 85 (7) ◽  
pp. 2065-2075 ◽  
Author(s):  
I. C. Livieratos ◽  
E. Eliasco ◽  
G. Müller ◽  
R. C. L. Olsthoorn ◽  
L. F. Salazar ◽  
...  
Keyword(s):  
Structure Prediction ◽  
Secondary Structure Prediction ◽  
Gene Array ◽  
Distinct Species ◽  
Trialeurodes Vaporariorum ◽  
Yellow Vein ◽  
Northern Hybridization ◽  
Stem Loop ◽  
Reading Frame ◽  
Potato Yellow Vein Virus

Double-stranded RNA preparations produced from potato plants graft-inoculated with a Peruvian isolate of Potato yellow vein virus (PYVV; genus Crinivirus, family Closteroviridae) contain five RNA species denoted RNA 1, RNA 2, RNA 3, x and y of approximately 8, 5·3, 3·8, 2·0 and 1·8 kbp, respectively. The complete nucleotide sequences of PYVV RNAs 1, 2 and 3 and Northern hybridization analysis showed that PYVV RNA 1 contained the replication module and an additional open reading frame (p7), while two distinct species, RNAs 2 and 3, contain the Closteroviridae hallmark gene array. Pairwise comparisons and phylogeny of genome-encoded proteins showed that PYVV shares significant homology with other criniviruses but is most closely related to the Trialeurodes vaporariorum-vectored Cucumber yellows virus. Secondary structure prediction of the 3′-untranslated regions of all three PYVV RNAs revealed four conserved stem–loop structures and a 3′-terminal pseudoknot structure, also predicted for all fully characterized members of the genus Crinivirus and some members of the genera Closterovirus and Ampelovirus.

scholarly journals Molecular insights on potato yellow vein crinivirus infections in the highlands of Colombia

2021 ◽  
Vol 102 (6) ◽  
Author(s):  
Ángela Niño ◽  
Francisco J. del Toro ◽  
Francisco Tenllado ◽  
Tomás Canto ◽  
Liliana Franco-Lara
Keyword(s):  
High Throughput Sequencing ◽  
Asymptomatic Infection ◽  
Yellow Vein ◽  
Rt Pcr ◽  
Genomic Rnas ◽  
Complete Sequences ◽  
Genomic Regions ◽  
Potato Yellow Vein Virus ◽  
Geographical Locations ◽  
Viral Isolates

Potato yellow vein virus (PYVV) was detected in potatoes grown in the Central highlands, north of Bogotá (~3000 m altitude), Colombia. At this altitude viral whitefly vectors are largely absent, but infection persists because of the use of uncertified tubers. Plants with typical PYVV-induced yellowing symptoms, as well as with atypical yellowing or non-symptomatic symptoms were sampled at three separate geographical locations. PYVV presence was assessed by RT-PCR, and several plants were subjected to high-throughput sequencing (HTS) of their small RNA (sRNA) populations. Complete or almost complete sequences of four PYVV isolates were thus reconstructed, all from symptomatic plants. Three viral isolates infected plants singly, while the fourth co-infected the plant together with a potyvirus. Relative proportions of sRNAs to each of the three crinivirus genomic RNAs were found to remain comparable among the four infections. Genomic regions were identified as hotspots of sRNA formation, or as regions that poorly induced sRNAs. Furthermore, PYVV titres in the mixed versus single infections remained comparable, indicating an absence of synergistic/antagonistic effects of the potyvirus on the accumulation of PYVV. Daughter plants raised in the greenhouse from tubers of the infected, field-sampled plants displayed mild PYVV infection symptoms that disappeared with time, demonstrating the occurrence of recovery and asymptomatic infection phenotypes in this pathosystem.

Sign in / Sign up

Export Citation Format

Share Document