scholarly journals Molecular characterisation of infectious bursal disease virus (IBDV) isolated from commercial broiler chickens in Nile Delta

2019 ◽  
Vol 22 (4) ◽  
pp. 399-408 ◽  
Author(s):  
N. Alkhalefa ◽  
M. El-Abasy ◽  
S. Kasem ◽  
E. Abu El-Naga
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Broiler Chickens ◽  
Nile Delta ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Nucleotide Sequence Analysis ◽  
Vp2 Gene ◽  
Bursal Disease ◽  
Commercial Broiler

Infectious bursal disease virus (IBDV) is a highly infectious disease affecting young chickens that alters predominantly the immune system. Emergence of new variants causes severe economic losses not only in Egypt but also all over the world. For this purpose assessment of infectious bursal disease virus (IBDV) genotypes in 20 commercial broiler flocks aged 20–35 days raised in 5 provinces in the Nile Delta, Egypt (Gharbia, Dakahlya, Kafr El sheikh, Zagazig and Domietta) was carried out. All flocks were vaccinated against IBD virus. RT-PCR revealed successful amplification of 620 bp of VP2 in 17 out of 20 samples (85%). VP2 gene nucleotide sequence analysis of six IBDV isolates (F342-1, F342-2, F342-4, F342-5 and F342-7) revealed 99.1 % similarity to the Giza 2000, Giza 2008 vv, SV-G1, SV-G2, SV-G4 and SV-G5 which were very virulent IBDV strains while the isolate F342-3 was close to D78 classical vaccinal strain and Kal 2001 classical IBDV strain variant.

Detection of very virulent infectious bursal disease virus from a field outbreak in Central India

2012 ◽  
Vol 60 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Azad Singh ◽  
Megha Bedekar ◽  
Rakesh Sharma ◽  
Bikash Sarkhel ◽  
Sanjeev Singh ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Clinical Signs ◽  
Central India ◽  
Infectious Bursal Disease ◽  
Nucleotide Sequence Analysis ◽  
Nucleotide Polymorphisms ◽  
Vp2 Gene ◽  
Bursal Disease ◽  
Commercial Broiler

In order to detect infectious bursal disease virus (IBDV), bursal tissue was collected from 10 IBD-suspected birds from a 30-day-old, IBDV-vaccinated commercial broiler chicken flock of 2000 birds exhibiting clinical signs suggestive of infectious bursal disease (IBD). The presence of IBDV was confirmed by partial amplification of the VP2 gene by reverse transcription and polymerase chain reaction. Isolates were identified as very virulent strains of IBDV (vvIBDV) by nucleotide sequence analysis. The comparison of the VP2 nucleotide sequences among the isolates revealed the presence of single-nucleotide polymorphisms in the VP2 gene of IBDV in the same flock. The comparative analysis indicated that these viruses were genetically close to the vvIBDVs previously detected in India. Our analysis provided information about the existence of vvIBDV in Central India.

scholarly journals Sequence diversity and evolution of infectious bursal disease virus in Iraq

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 293
Author(s):  
Ali Hadi Abbas ◽  
Haider Abas AL saegh ◽  
Furkan Sabbar ALaraji
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Sequence Similarity ◽  
Sequence Diversity ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Vp2 Gene ◽  
Vaccination Programs ◽  
Geographical Regions ◽  
Bursal Disease

Background: Infectious Bursal Disease (IBD) is a highly infectious disease which causes huge economic losses to the poultry industry due to the direct impact of the illness and indirect consequences such as decreasing the general immunity of the flock, leaving it naive to other diseases. In Iraq, IBD is highly prevalent despite vaccination programs, yet studies on sequence diversity of the causative virus are still rare.  Methods: A sample from Bursa of Fabricius from an IBD outbreak in a flock in the city of Najaf in Iraq was smeared on an FTA card. Amplicons of targeted regions in VP1 and VP2 genes were generated and sequenced. Sequences were then compared with other local and global sequences downloaded from GenBank repositories. Sequence alignment and DNA sequence analyses were achieved using MUSCLE, UGENE and MEGAx software. The molecular clock and sequence evolutionary analyses were applied using MEGAx tools.  Results: The strain sequenced in this study belongs to a very virulent Infectious Bursal Disease Virus (vvIBDV) as the DNA and phylogenetic analysis of VP1 and VP2 gene sequences showed a mutual clustering with similar sequences belonging to vvIBDV genogroup 3. Analyses of the hyper variable region of VP2 gene (hvVP2) of IBDV isolates from Iraq indicates a presence of sequence diversity. Interestingly, the two vaccine strains Ventri IBDV Plus and ABIC MB71 that showed the highest sequence similarity to the local isolates in the hvVP2 region are not used in vaccination routine against IBDV in Iraq.  Conclusion: Sequences of vvIBDV in Iraq are diverse. Remarkably, some of the available vaccine strains show high sequence similarity with local strains in Iraq; however, they are not included in the routine vaccination programs. Analysis of more samples involving more geographical regions is needed to draw a detailed map of antigenic diversity of IBDV in Iraq.

scholarly journals Molecular Characterization of Infectious Bursal Disease Virus Isolated from Naturally Infected Broiler Chickens in Erbil, Iraq

2020 ◽  
Vol 44 ((E0)) ◽  
pp. 21-27
Author(s):  
Ahmed I. Ahmed
Keyword(s):  
Molecular Characterization ◽  
Broiler Chickens ◽  
Hypervariable Region ◽  
Chorioallantoic Membrane ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Nucleotide Sequence Analysis ◽  
Vp2 Gene ◽  
Bursal Disease

The infectious bursal disease (IBD) is a highly contagious and immunosuppressive disease of broiler chickens and the development of a new genetic variant of the virus is responsible for major economic losses in the poultry industry. For this purpose, it was essential to isolate the molecular characterization of the virus from vaccinated broiler in Erbil, Iraq. Clinically, the infectious bursal disease is characterized by high mortality (10-15%) with hemorrhagic lesions on the breast and thigh muscles, hemorrhagic and edematous bursa of diseased chickens. In this study, the Bursa of Fabricus (BF) samples were collected between June 2018 and January 2019. Histopathological changes of the bursal sections showed existence of the cystic vacuolation of the lymphoid follicles with leukocytes infiltration as pathognomic features for IBD virus infection; and homogenates samples inoculated in chorioallantoic-membrane showed mortality in the second passage with varying degrees of hemorrhages. Agar gel precipitation test (AGPT) was positive with specific antisera. Reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequence analysis of five fragments in the hypervariable region of VP2 gene revealed transition and transversion changes. Among the five recent IBD virus isolates, the rate of identity was approximately 99% as compared with the very virulent IBD virus from Iran (ID: DQ785171.1). Phylogenetic analysis revealed that the five isolates were closely related to the Asian group with a different percentage ranged from 98-99% while it was 97% in the European group. The local isolate of the virus was registered in the Genebank under the accession number MN48052.1. In conclusion, the isolated IBDVs belong to a very virulent group. In addition, this study demonstrates the spread of this virulent virus to poultry industries in Erbil, Iraq. Further widespread surveys could help in delivering more information on the virus variability and might assist in designing novel vaccines for this pathogen

scholarly journals Sequence diversity and evolution of infectious bursal disease virus in Iraq

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 293
Author(s):  
Ali Hadi Abbas ◽  
Haider Ali AL saegh ◽  
Furkan Sabbar ALaraji
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Sequence Similarity ◽  
Sequence Diversity ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Vp2 Gene ◽  
Vaccination Programs ◽  
Geographical Regions ◽  
Bursal Disease

Background: Infectious Bursal Disease (IBD) is a highly infectious disease which causes huge economic losses to the poultry industry due to the direct impact of the illness and indirect consequences such as decreasing the general immunity of the flock, leaving it naive to other diseases. In Iraq, IBD is highly prevalent despite vaccination programs, yet studies on sequence diversity of the causative virus are still rare.  Methods: A sample from Bursa of Fabricius from an IBD outbreak in a flock in the city of Najaf in Iraq was smeared on an FTA card. Amplicons of targeted regions in VP1 and VP2 genes were generated and sequenced. Sequences were then compared with other local and global sequences downloaded from GenBank repositories. Sequence alignment and DNA sequence analyses were achieved using MUSCLE, UGENE and MEGAx software. The molecular clock and sequence evolutionary analyses were applied using MEGAx tools.  Results: The strain sequenced in this study belongs to a very virulent Infectious Bursal Disease Virus (vvIBDV) as the DNA and phylogenetic analysis of VP1 and VP2 gene sequences showed a mutual clustering with similar sequences belonging to vvIBDV genogroup 3. Analyses of the hyper variable region of VP2 gene (hvVP2) of IBDV isolates from Iraq indicates a presence of sequence diversity. Interestingly, the two vaccine strains Ventri IBDV Plus and ABIC MB71 that showed the highest sequence similarity to the local isolates in the hvVP2 region are not used in vaccination routine against IBDV in Iraq.  Conclusion: Sequences of vvIBDV in Iraq are diverse. Remarkably, some of the available vaccine strains show high sequence similarity with local strains in Iraq; however, they are not included in the routine vaccination programs. Analysis of more samples involving more geographical regions is needed to draw a detailed map of antigenic diversity of IBDV in Iraq.

Sign in / Sign up

Export Citation Format

Share Document