Molecular characterisation of lumpy skin disease virus and sheeppox virus based on P32 gene

Vaccines form the cornerstone of any control, eradication and preventative strategy and this is no different for lumpy skin disease. However, the usefulness of a vaccine is determined by a multiplicity of factors which include stability, efficiency, safety and ease of use, to name a few. Although the vaccination campaign in the Balkans against lumpy skin disease virus (LSDV) was successful and has been implemented with success in the past in other countries, data of vaccine failure have also been reported. It was therefore the purpose of this study to compare five homologous live attenuated LSDV vaccines (LSDV LAV) in a standardized setting. All five LSDV LAVs studied were able to protect against a challenge with virulent LSDV. Aside from small differences in serological responses, important differences were seen in side effects such as a local reaction and a Neethling response upon vaccination between the analyzed vaccines. These observations can have important implications in the applicability in the field for some of these LSDV LAVs.

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Production of small ruminant morbillivirus, rift valley fever virus and lumpy skin disease virus in CelCradle™ -500A bioreactors

BMC Veterinary Research ◽

10.1186/s12917-021-02801-4 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Halima Rhazi ◽

Najete Safini ◽

Karima Mikou ◽

Meryeme Alhyane ◽

Khalid Omari Tadlaoui ◽

...

Keyword(s):

Disease Virus ◽

Skin Disease ◽

Rift Valley ◽

Rift Valley Fever ◽

Rift Valley Fever Virus ◽

Fever Virus ◽

Valley Fever ◽

Lumpy Skin Disease ◽

Lumpy Skin Disease Virus ◽

Cell Factories

Abstract Background Animal vaccination is an important way to stop the spread of diseases causing immense damage to livestock and economic losses and the potential transmission to humans. Therefore effective method for vaccine production using simple and inexpensive bioprocessing solutions is very essential. Conventional culture systems currently in use, tend to be uneconomic in terms of labor and time involved. Besides, they offer a limited surface area for growth of cells. In this study, the CelCradle™-500A was evaluated as an alternative to replace conventional culture systems in use such as Cell factories for the production of viral vaccines against small ruminant morbillivirus (PPR), rift valley fever virus (RVF) and lumpy skin disease virus (LSD). Results Two types of cells Vero and primary Lamb Testis cells were used to produce these viruses. The study was done in 2 phases as a) optimization of cell growth and b) virus cultivation. Vero cells could be grown to significantly higher cell densities of 3.04 × 109 using the CelCradle™-500A with a shorter doubling time as compared to 9.45 × 108 cells in Cell factories. This represents a 19 fold increase in cell numbers as compared to seeding vs only 3.7 fold in Cell factories. LT cells achieved modestly higher cell densities of 6.7 × 108 as compared to 6.3 × 108 in Cell factories. The fold change in densities for these cells was 3 fold in the CelCradle™-500A vs 2.5 fold in Cell factories. The titers in the conventional system and the bioreactor were not significantly different. However, the Cell-specific virus yield for rift valley fever virus and lumpy skin disease virus are higher (25 virions/cell for rift valley fever virus, and 21.9 virions/cell for lumpy skin disease virus versus 19.9 virions/cell for rift valley fever virus and 10 virions/cell for lumpy skin disease virus). Conclusions This work represents a novel study for primary lamb testis cell culture in CellCradle™-500A bioreactors. In addition, on account of the high cell densities obtained and the linear scalability the titers could be further optimized using other culture process such us perfusion.

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Molecular characterization of lumpy skin disease virus in Iran (2014–2018)

Archives of Virology ◽

10.1007/s00705-021-05119-6 ◽

2021 ◽

Author(s):

Zeinab Hedayati ◽

Hamid Reza Varshovi ◽

Ali Mohammadi ◽

Mohammad Tabatabaei

Keyword(s):

Molecular Characterization ◽

Disease Virus ◽

Skin Disease ◽

Lumpy Skin Disease ◽

Lumpy Skin Disease Virus

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Sporadic cases of lumpy skin disease among cattle in Sharkia province, Egypt: Genetic characterization of lumpy skin disease virus isolates and pathological findings

Veterinary World ◽

10.14202/vetworld.2018.1150-1158 ◽

2018 ◽

Vol 11 (8) ◽

pp. 1150-1158 ◽

Cited By ~ 7

Author(s):

Fatma M. Abdallah ◽

Hend M. El Damaty ◽

Gamilat F. Kotb

Keyword(s):

Disease Virus ◽

Skin Disease ◽

Genetic Characterization ◽

Pathological Findings ◽

Lumpy Skin Disease ◽

Lumpy Skin Disease Virus ◽

Sporadic Cases ◽

Virus Isolates

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Cutaneous lymphoma in a cow - a case report

Veterinarski glasnik ◽

10.2298/vetgl180904004k ◽

2019 ◽

Vol 73 (1) ◽

pp. 50-56

Author(s):

Branislav Kureljusic ◽

Slobodan Maksimovic ◽

Slobodan Vujinovic ◽

Bozidar Savic ◽

Vesna Milicevic ◽

...

Keyword(s):

Disease Virus ◽

Skin Disease ◽

Mononuclear Cells ◽

Subcutaneous Tissue ◽

Leukemia Virus ◽

Surgical Excision ◽

Cutaneous Lymphoma ◽

Histopathological Analysis ◽

Lumpy Skin Disease ◽

Lumpy Skin Disease Virus

In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.

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Retention of Lumpy Skin Disease Virus in Stomoxys Spp (Stomoxys Calsitrans, Stomoxys Sitiens, Stomoxys Indica) following intrathoracic inoculation, Diptera: Muscidae

10.1101/2020.08.13.249227 ◽

2020 ◽

Author(s):

Arman Issimov ◽

Lespek Kutumbetov ◽

Assylbek Zhanabayev ◽

Nurlybay Kazhgaliyev ◽

Birzhan Nurgaliyev ◽

...

Keyword(s):

Disease Virus ◽

Skin Disease ◽

Virus Isolation ◽

Emerging Disease ◽

Lumpy Skin Disease ◽

Lumpy Skin Disease Virus ◽

Laboratory Conditions ◽

Means Of Transmission ◽

Intrathoracic Inoculation

AbstractLumpy skin disease (LSD) is an emerging disease in cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, acquisition of Lumpy Skin Disease Virus (LSDV) by Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. Flies were injected with a virulent LSDV strain into the thorax region to bypass the midgut barrier. The fate of pathogen in the hemolymph of the flies was further examined using PCR and Virus isolation tests. LSDV was isolated from all three Stomoxys species immediately and up to 24h post intrathoracic inoculation while virus DNA was detectable up to 7d post intrathoracic inoculation.

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