Characterization of potential plant growth-promoting rhizobacteria isolated from
            sunflower (Helianthus annuus L.) in Iran

Purpose This study aims to characterize plant growth-promoting rhizobacteria (PGPR) in sunflowers growing in different locations at North West of Iran. Materials and methods Sunflower plants were collected from different regions of West Azarbaijan, and rhizospheric bacterial strains were isolated and screened for PGP traits. Identification and characterization of the PGPR were conducted based on 16s rDNA sequences and phenotypic analysis, the strains clustered for genetic diversity by rep-PCR method. Results Among the 80 bacterial isolates, 20 showed PGP traits and were selected for other potentials. All the selected isolates produced indole-3-acetic acid at the rate of 9.2–33.7 mg/ml. In addition, 13, 15, 12, and 16 were positive for phosphate solubilization, siderephore, hydrogen cyanide, and ammonia production, respectively. The results from a subsequent pot experiment indicated that PGPRs distinctly increased sun flower shoot and root length, shoot and root fresh weight, as well as shoot and root dry weight. Based on 16S rDNA sequences and biochemical and physiological characteristics, 20 PGPRs were identified as Pseudomonas fluorescens (five isolates), Pseudomonas aeruginosa (four isolates), Pseudomonas geniculata (one isolate), Bacillus subtilis (four isolates), Bacillus pumilus (two isolates), Stenotrophomonas maltophilia (two isolates), and Brevibacterium frigoritolerans (two isolates). In rep-PCR, PGPR isolates were differentiated into seven clusters (A–G) at 65% similarity level. These results demonstrated the existence of a considerable species richness and genetic diversity among PGPRs isolated from different regions of North West of Iran. Conclusions To the best of our knowledge, this is first report for the identification and characterization of B. frigoritolerans as PGPR in sunflower plants.

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Molecular Tools for Identification and Characterization of Plant Growth Promoting Rhizobacteria with Emphasis in Azospirillum spp.

Handbook for Azospirillum ◽

10.1007/978-3-319-06542-7_2 ◽

2015 ◽

pp. 27-44 ◽

Cited By ~ 1

Author(s):

Chiu-Chung Young ◽

Shih-Yao Lin ◽

Fo-Ting Shen ◽

Wei-An Lai

Keyword(s):

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Molecular Tools ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Identification And Characterization

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Characterization of Bacteria Isolated from the Saffron (Crocus sativus L.) Rhizosphere

Journal of Horticultural Research ◽

10.1515/johr-2017-0017 ◽

2017 ◽

Vol 25 (2) ◽

pp. 5-14

Author(s):

Majid Jami Al-Ahmadi ◽

Abbas Mohammadi ◽

Esmaeil Salehi Kohabadi

Keyword(s):

Dry Weight ◽

Plant Growth Promoting Rhizobacteria ◽

Crocus Sativus ◽

Phenotypic Traits ◽

Rdna Sequences ◽

16S Rdna Sequences ◽

Plant Growth Promoting ◽

Sequences Analysis ◽

Crocus Sativus L

Abstract One purpose of assessing the soil alive and active community is the identification of beneficial bacteria to use them as biological fertilizers, replacing or supplementing synthetic fertilizers. Such biofertilizers are predicted for the sustainability of agricultural production, especially for low input systems such as saffron fields. The aim of this work was to isolate and identify saffron rhizobacteria and to evaluate their possible effects on saffron growth. During 2013/14, some bacteria were isolated from the rhizosphere of the saffron plantations of different age in Gol village, Birjand, Iran. In total, 12 bacteria species were identified based on phenotypic traits and 16S rDNA sequences analysis. The strains were identified as B. subtilis, B. anthracis, B. cereus, B. megaterium, Bacillus sp., Paenibacillus, Pseudomonas fluorescens, P. putida, Escherichia coli, Pectobacterium sp. and Pantoea sp., with the dominant population belonging to the genus Bacillus. In the field study, inoculation of soil with these strains did not affect the leaf dry weight of the cultivated saffron, however, the strains of P. fluorescens increased the leaf area while P. fluorescens, Paenibacillus, Pectobacterium and B. megaterium increased the number of daughter corms and Azotobacter, B. cereus, B. subtilis and B. megaterium increased the corm weight. Our finding revealed that some bacteria present in the soil of perennial saffron plantations have a promising potential for developing as a plant growth promoting rhizobacteria.

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