DR3 regulation of apoptosis of naive T-lymphocytes in children with acute infectious mononucleosis

Atypical lymphocytes from nine young adults with acute infectious mononucleosis (IM) were studied for morphologic, ultrastructural, cytochemical, and membrane surface marker characteristics. There was an absolute increase in T lymphocytes in the patients. Atypical lymphocytes accounted for 83%-96% of the lymphocyte population. These lymphocytes contained cytoplasmic inclusions which ranged in size from 1000 to 6000 A, were usually membrane bound, and consisted of parallel arrays of microtubulelike structures. The inclusions, which have been referred to as parallel tubular arrays (PTA), were found in 15%-75% of the lymphocytes from the IM patients. Ultrastructural cytochemical methods demonstrated acid phosphatase activity within many of the membrane-bound PTA. The function of the PTA is unknown. Since they were observed only in the lymphocytes which appeared to correspond to the atypical lymphocytes on light microscopy, the majority of which typed as T cells, there appears to be an association between PTA and T lymphocytes. It is possible that PTA identify a specific subset of T lymphocytes which is expanded in IM. Alternatively, PTA may be a transient finding in lymphocytes appearing only in certain biologic states of the cell such as during T-lymphocyte activation.

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Assessment of Apoptosis Level of Naive CD8+ T-lymphocytes in Children with Acute Infectious Mononucleosis in CD95 and DR3 Receptors Activation

Sovremennye tehnologii v medicine ◽

10.17691/stm2015.7.3.16 ◽

2015 ◽

Vol 7 (3) ◽

pp. 109-118 ◽

Cited By ~ 1

Author(s):

E.N. Filatova ◽

O.V. Utkin ◽

E.V. Anisenkova ◽

N.B. Presnyakova ◽

T.D. Sycheva ◽

...

Keyword(s):

T Lymphocytes ◽

Infectious Mononucleosis ◽

Cd8 T Lymphocytes ◽

Acute Infectious Mononucleosis ◽

Apoptosis Level

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Ultrastructural, cytochemical, and membrane surface marker characteristics of the atypical lymphocytes in infectious mononucleosis

Blood ◽

10.1182/blood.v50.3.505.505 ◽

1977 ◽

Vol 50 (3) ◽

pp. 505-515 ◽

Cited By ~ 14

Author(s):

RW McKenna ◽

J Parkin ◽

KJ Gajl-Peczalska ◽

JH Kersey ◽

RD Brunning

Keyword(s):

T Lymphocytes ◽

Infectious Mononucleosis ◽

Membrane Surface ◽

Lymphocyte Activation ◽

Surface Marker ◽

Absolute Increase ◽

Cytoplasmic Inclusions ◽

Specific Subset ◽

Membrane Bound ◽

Acute Infectious Mononucleosis

Abstract Atypical lymphocytes from nine young adults with acute infectious mononucleosis (IM) were studied for morphologic, ultrastructural, cytochemical, and membrane surface marker characteristics. There was an absolute increase in T lymphocytes in the patients. Atypical lymphocytes accounted for 83%-96% of the lymphocyte population. These lymphocytes contained cytoplasmic inclusions which ranged in size from 1000 to 6000 A, were usually membrane bound, and consisted of parallel arrays of microtubulelike structures. The inclusions, which have been referred to as parallel tubular arrays (PTA), were found in 15%-75% of the lymphocytes from the IM patients. Ultrastructural cytochemical methods demonstrated acid phosphatase activity within many of the membrane-bound PTA. The function of the PTA is unknown. Since they were observed only in the lymphocytes which appeared to correspond to the atypical lymphocytes on light microscopy, the majority of which typed as T cells, there appears to be an association between PTA and T lymphocytes. It is possible that PTA identify a specific subset of T lymphocytes which is expanded in IM. Alternatively, PTA may be a transient finding in lymphocytes appearing only in certain biologic states of the cell such as during T-lymphocyte activation.

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Inflammatory activity in the cerebrospinal fluid a decade after acute infectious mononucleosis

10.26226/morressier.5b75785c5aff7400151f3aaa ◽

2018 ◽

Author(s):

Daniel Jons ◽

Oluf Andersen

Keyword(s):

Cerebrospinal Fluid ◽

Infectious Mononucleosis ◽

Inflammatory Activity ◽

Acute Infectious Mononucleosis

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The vagaries of IgM: a case report of EBV infection with concomitantly false-positive IgM for CMV, VZV, and HSV

The Egyptian Journal of Internal Medicine ◽

10.1186/s43162-020-00006-z ◽

2020 ◽

Vol 32 (1) ◽

Author(s):

Raai Mahmood ◽

Khalid Mohamed ◽

Naba Saeed ◽

Kadhim Al-Banaa ◽

Jonathan Zimmerman ◽

...

Keyword(s):

Infectious Mononucleosis ◽

Total Bilirubin ◽

Viral Infections ◽

Total Bilirubin Level ◽

Peripheral Blood Smear ◽

Cross Reactivity ◽

Immunoglobulin M ◽

Case Presentation ◽

Ebv Infection ◽

Acute Infectious Mononucleosis

Abstract Background Serum IgM (immunoglobulin M) testing is commonly used to diagnose acute viral infections. However, most clinicians are unaware of the vagaries of IgM testing, including antigenic cross-reactivity between multiple viruses and risk misdiagnosis. Case presentation We report a case of infectious mononucleosis with concomitantly positive IgM for EBV, CMV, VZV, and HSV. A 26-year-old man presented with acute infectious mononucleosis picture. His blood work showed a total bilirubin level of 7.7 mg/dl, ALT 1077 U/L, AST 806 U/L, ALP 325 U/L, and INR 1.0. Monospot was positive; peripheral blood smear showed atypical lymphocytes; however, because EBV infectious mononucleosis does not typically cause elevation of liver enzymes over 1000, other etiologies were explored. Tests for hepatitis A, B, C, HIV, ANA, and ASMA returned negative. IgM for EBV-VCA, CMV, HSV, and VZV all returned positive, and the diagnosis of EBV IM was called into question. Subsequent tests of CMV and HSV PCR for viral load were negative (VZV was not clinically suspected), and later on, EBV-EBNA returned negative and EBV-VCA IgM and IgG returned positive, confirming the diagnosis of acute EBV infection. Conclusion We believe that IgM seropositivity can result from cross-reactivity among several viruses (especially herpes viruses), and although often relied on, a positive IgM should not serve as the sole determinant for diagnosis of acute viral infections.

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