scholarly journals Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

2018 ◽  
Vol 30 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Katarina Rudolf Pilih ◽  
Urška Karolina Potokar ◽  
Borut Bohanec
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Petri Dish ◽  
Abnormal Development ◽  
Haploid Induction ◽  
Embryo Formation ◽  
White Cabbage ◽  
Dh Lines ◽  
Further Development

Abstract Protocols leading to the development of doubled haploid (DH) lines by microspore culture are widely used in white cabbage (Brassica oleracea var. capitata L.), but efficiency varies according to the cultivar and induction procedure. Forty different genotypes consisting of F1 cultivars and their crosses with responsive doubled haploid lines were tested to evaluate the androgenic response. In total, 20,032 embryos were produced. On average, the haploid induction response of F1 cultivars was 7.0 embryos/Petri dish, but the average of these hybrids crossed to responsive DH lines was 26.6 embryos/Petri dish. In seven reciprocal crosses, a difference was observed in just one, meaning that the maternal effect probably has a minor influence on haploid embryogenesis in cabbage. Addition of 0.02% activated charcoal (AC) to the induction media increased embryo formation in several low-responsive genotypes, but its effect on embryo formation of high-responsive genotypes was predominantly negative, although larger embryos were formed on media containing AC than without AC. Further development into plantlets was tested by two procedures. Formed embryos were either transferred directly to regeneration medium or treated with abscisic acid and desiccated for 4 weeks. Regrowth and further development reached on average 15.5 and 57.6%, for the first and second procedures, respectively. Plantlets developed by direct transfer often exhibited abnormal development or hyperhydricity, unlike the desiccated embryos. Spontaneous diploidisation of embryos reached 42.5% in total and was not affected by AC added to the induction media.

scholarly journals The Influence of pH on Microspore Embryogenesis of White Cabbage (Brassica oleracea L.)

2013 ◽  
Vol 5 (4) ◽  
pp. 485-489 ◽  
Author(s):  
Tina Oana CRISTEA
Keyword(s):  
Brassica Oleracea ◽  
Microspore Culture ◽  
Microspore Embryogenesis ◽  
Critical Factor ◽  
Cellular Level ◽  
Strong Base ◽  
White Cabbage ◽  
In Vitro Microspore Culture ◽  
Influence Of Ph

In vitro microspore culture is one of the top techniques utilised now-a-days for the obtaining of double haploid plants in many plant species, including Brassica. The pH of the medium is a critical factor for the success of In vitro microspore culture as it influences the invertase enzyme activity, translated at cellular level through an acceleration or reduction of sucrose cleavage. The results published until now shows rather contradictory findings, as the response of microspores have been proved to be highly depending on genotypes, most of them being focused on Brassica napus. Thus, in the present study, the effect of different NLN liquid medium pH, ranging between 5.0 to 7.0 were tested in order to establish the most suitable pH for the expression of embryogenic competences of microspores cultivated on medium In vitro and ultimately for the obtaining of microspore-derived embryos. Among the 11 values of pH tested, the best results were obtained on variants with pH 5.8 and 6.0, both in what concern the maintaining of microspores viability and the number of microspore-derived embryos. The findings of the present study provide a strong base for the establishment of an efficient protocol for the In vitro culture of microspore at Brassica oleracea L. genotypes with Romanian origin.

scholarly journals Tradition, quality and biotechnology in Hungarian spice pepper ( Capsicum annuum L.) breeding

2010 ◽  
Vol 58 (3) ◽  
pp. 259-266 ◽  
Author(s):  
J. Pauk ◽  
C. Lantos ◽  
G. Somogyi ◽  
P. Vági ◽  
Z. Ábrahám Táborosi ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Culture Methods ◽  
Culture Techniques ◽  
New Genotype ◽  
Breeding Programmes ◽  
History Of ◽  
Dh Lines ◽  
Two Hybrid

Spice pepper production has a history of almost 300 years in the southern part of Hungary. In this study the results of two biotechnological improvements are summarized. Anther and isolated microspore culture techniques were improved to release haploid and doubled haploid (DH) lines for spice pepper breeding. Both the anther and isolated microspore culture methods were successfully used in spice pepper haploid production. Microspore culture-derived structures were analysed to identify their different parts. Green plantlets were regenerated from embryos derived from both anther and microspore cultures. Their doubled haploid analogues were integrated into Hungarian spice pepper hybrid seed breeding programmes. One hybrid, Sláger, was released as a new genotype for spice pepper production in 2008 and two hybrid candidates (Délibáb and Bolero) are now being tested in official trials.

Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis)

Euphytica ◽  
2013 ◽  
Vol 195 (3) ◽  
pp. 467-475 ◽  
Author(s):  
Honghui Gu ◽  
Zhengqing Zhao ◽  
Xiaoguang Sheng ◽  
Huifang Yu ◽  
Jiansheng Wang
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Haploid Production

scholarly journals Influence of different wheat and Imperata cylindrica genetic backgrounds on haploid induction efficiency in wheat doubled haploid breeding

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 195-200 ◽  
Author(s):  
S.A. Rather ◽  
H.K. Chaudhary ◽  
V. Kaila
Keyword(s):  
Bread Wheat ◽  
Doubled Haploid ◽  
Combining Ability ◽  
General Combining Ability ◽  
Imperata Cylindrica ◽  
Haploid Induction ◽  
Embryo Formation ◽  
Haploid Production ◽  
Formation Efficiency ◽  
Doubled Haploid Breeding

Four Indian and one Japanese accession of Imperata cylindrica were assessed for their influence upon haploid production in F<sub>1</sub> generations of 21 wheat crosses (winter &times; spring, spring &times; spring and winter &times; winter) to find an efficient pollen source for haploid induction, which would enhance doubled haploid breeding in bread wheat. The frequency of haploid induction was influenced differently by the wheat and the I. cylindrica genotypes, indicating both maternal and paternal genetic influence on haploid induction. The gene actions controlling the inheritance of&nbsp; haploid induction&nbsp; appeared to be non-additive. Haploid formation efficiency was closely associated with other haploid induction parameters, i.e. pseudoseed formation, embryo formation and haploid regeneration. Amongst wheat F<sub>1</sub> groups, spring &times; spring wheats exhibited the highest potential for haploid induction. General combining ability for haploid production was highest for the, I. cylindrica genotype Ic-Aru, native to the northeastern Himalayas, which appears as a potential pollen source for efficient haploid induction in bread wheat.

scholarly journals Evaluation of Gynogenic Responsiveness and Pollen Viability of Selfed Doubled Haploid Onion Lines and Chromosome Doubling via Somatic Regeneration

2010 ◽  
Vol 135 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Marijana Jakše ◽  
Pablo Hirschegger ◽  
Borut Bohanec ◽  
Michael J. Havey
Keyword(s):  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Shoot Culture ◽  
Haploid Induction ◽  
Flower Bud ◽  
Breeding Programs ◽  
Regeneration Stage ◽  
Regeneration Procedure ◽  
Dh Lines

Although haploid induction has been used in onions (Allium cepa L.) for over 20 years, several obstacles limit its use in plant breeding programs. To address these limitations, we evaluated the responsiveness of doubled haploid (DH) lines and their selfed progenies, an alternative protocol for chromosome doubling using somatic regeneration of haploid lines, and pollen viability of DH lines. Twenty-one DH lines were self pollinated and tested for haploid induction in the second generation. Among the DH lines, 18 lines showed an average of 20% decrease in gynogenic responsiveness compared with the original lines, while three lines registered an average increase of 5.7%. Using a two-step induction/regeneration procedure, 8,589 somatic regenerants were obtained from 16,170 flower buds from haploid plants, and shoot culture was established. A more laborious procedure using extraction of ovaries in the regeneration stage was found equal to flower bud culture. Chromosome doubling via somatic regeneration was found to be 83% and 100% efficient when the source material was haploid or mixoploid, respectively. Based on the results achieved in this and previous studies, an alternative protocol for chromosome doubling of gynogenic haploids is proposed.

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

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