Doubled Haploid Production in High- and Low-Response Genotypes of Rapeseed (Brassica napus) Through Isolated Microspore Culture

2021 ◽  
pp. 129-144
Author(s):  
Patricia Corral-Martínez ◽  
Carolina Camacho-Fernández ◽  
Ricardo Mir ◽  
Jose M. Seguí-Simarro
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Haploid Production

scholarly journals RETRACTED ARTICLE: Doubled haploid production in alfalfa (Medicago sativa L.) through isolated microspore culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dengxia Yi ◽  
Jifeng Sun ◽  
Yanbin Su ◽  
Zongyong Tong ◽  
Tiejun Zhang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Retracted Article ◽  
Medicago Sativa L

scholarly journals Retraction Note: Doubled haploid production in alfalfa (Medicago sativa L.) through isolated microspore culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dengxia Yi ◽  
Jifeng Sun ◽  
Yanbin Su ◽  
Zongyong Tong ◽  
Tiejun Zhang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Medicago Sativa L

This paper has been retracted.

Maternal doubled haploid production in interploidy hybridization between Brassica napus and Brassica allooctaploids

Planta ◽  
2017 ◽  
Vol 247 (1) ◽  
pp. 113-125 ◽  
Author(s):  
Shaohong Fu ◽  
Liqin Yin ◽  
Mingchao Xu ◽  
Yun Li ◽  
Maolin Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Haploid Production

scholarly journals Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
The Czech Republic ◽  
Yield Parameters ◽  
The World

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

scholarly journals Tradition, quality and biotechnology in Hungarian spice pepper ( Capsicum annuum L.) breeding

2010 ◽  
Vol 58 (3) ◽  
pp. 259-266 ◽  
Author(s):  
J. Pauk ◽  
C. Lantos ◽  
G. Somogyi ◽  
P. Vági ◽  
Z. Ábrahám Táborosi ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Culture Methods ◽  
Culture Techniques ◽  
New Genotype ◽  
Breeding Programmes ◽  
History Of ◽  
Dh Lines ◽  
Two Hybrid

Spice pepper production has a history of almost 300 years in the southern part of Hungary. In this study the results of two biotechnological improvements are summarized. Anther and isolated microspore culture techniques were improved to release haploid and doubled haploid (DH) lines for spice pepper breeding. Both the anther and isolated microspore culture methods were successfully used in spice pepper haploid production. Microspore culture-derived structures were analysed to identify their different parts. Green plantlets were regenerated from embryos derived from both anther and microspore cultures. Their doubled haploid analogues were integrated into Hungarian spice pepper hybrid seed breeding programmes. One hybrid, Sláger, was released as a new genotype for spice pepper production in 2008 and two hybrid candidates (Délibáb and Bolero) are now being tested in official trials.

scholarly journals Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 101-109 ◽  
Author(s):  
Olfa Slama-Ayed ◽  
Imen Bouhaouel ◽  
Sourour Ayed ◽  
Jacques De Buyser ◽  
Emmanuel Picard ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Microspore Culture ◽  
Plant Production ◽  
Haploid Plant ◽  
Interspecific Crosses ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Good Ability ◽  
Successful Approach

This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.  

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