Hyperdry amniotic membrane as a suitable biological  dressing material for raw wounds in the oral cavity

Background:Skin act as a protective barrier to prevent the contamination from environment and dehy- dration of tissue. After any surgery to the oral cavity, wound closure or reconstruction is mandatory which is done by skin graft and skin substitute like amniotic membrane by the process of epithelializa- tion and tissue regeneration. Amniotic membrane is use as skin substitute in burn, eye and oral cavity as biologic wound dressing.Objective:To evaluate the epithelialization after use of amniotic membrane in mucosal defect of oral cavity as a biologic dressing.Methods:This was a prospective study which included 26 with premalignant lesion in buccal mucosa who under went excision of the lesion from intraoral buccal mucosa between January 14-june 15 and post operative follow up for 4-6 month after the surgical procedure. We used amniotic membrane(am) for dressing of the defects in buccal mucosa of oral cavity under local anesthesia. Efficacy of this procedure was assessed by granulation tissue formation with surface epithelialization on the graft site.Results:The epithelialization evaluated in postoperative period. According to sex 40 males 70% and 30 females age 22-72 years with means age 45.0±10.5 years. After excision of lesion from buccal mucosa amniotic membrane was grafted on the defect. Completeadherence of amniotic membrane in most of the cases.Wound covered by epithelialization was entire wound coverage in 85% and Nearly entire wound coverage in 15% when use amniotic membrane use as a biologic dressing in oral cavity. No allergic reaction occurs in any patients.Conclusion: Amniotic membrane can be useas a skin substitute in buccal mucosa of the oral cavity.Update Dent. Coll. j: 2018; 8 (1): 36-40

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Maximizing the safety of glycerol preserved human amniotic membrane as a biological dressing

Burns ◽

10.1016/j.burns.2015.03.009 ◽

2015 ◽

Vol 41 (7) ◽

pp. 1498-1503 ◽

Cited By ~ 10

Author(s):

Serag M. Zidan ◽

Samy A. Eleowa ◽

Mahmoud A. Nasef ◽

Magdy A. Abd-Almoktader ◽

Amr M. Elbatawy ◽

...

Keyword(s):

Amniotic Membrane ◽

Human Amniotic Membrane ◽

Biological Dressing

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Amniotic membrane as a biological dressing in infected wound healing in rabbits

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502014000500008 ◽

2014 ◽

Vol 29 (5) ◽

pp. 334-339 ◽

Cited By ~ 5

Author(s):

Ian Göedert Leite Duarte ◽

Ivana Duval-Araujo

Keyword(s):

Wound Healing ◽

Amniotic Membrane ◽

Infected Wound ◽

Biological Dressing

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Use of Autologous Epithelium Transplantation on Various Scaffolds to Cover Tissue Loss in Oral Cavity: Long-Term Observation

Journal of Applied Biomaterials & Functional Materials ◽

10.5301/jabfm.5000312 ◽

2017 ◽

Vol 15 (1) ◽

pp. 25-30

Author(s):

Bogusława Orzechowska-Wylęgała ◽

Dariusz Dobrowolski ◽

Domenico Puzzolo ◽

Bogumił Wowra ◽

Wiktor Niemiec ◽

...

Keyword(s):

Tissue Engineering ◽

Oral Cavity ◽

Amniotic Membrane ◽

Cultured Cells ◽

Single Layer ◽

Tissue Loss ◽

Mucous Membranes ◽

Well Differentiated ◽

Long Term Observation

Background The aim of this study was to investigate the application of mucous membrane keratinocyte cultures on amniotic membrane and on poly(L-lactic acid) (PLLA) Purasorb PL38 to cover tissue loss in the oral cavity. Developments in molecular biology techniques and tissue engineering allow the culturing and identification of cells that can be anchored in the wound to achieve integrity of the tissue. Transplantation of tissues obtained from the patient's own cells is superior to allogenous transplantation where there is a possibility of transfection, rejection and the need for long-term immunosuppression. Methods In 9 patients (15 procedures) keratinocytes cultured on amniotic membrane and PLLA were transplanted to cover antro-oral fistulas and bone loss after osteoradionecrosis. Results In all 6 patients with outlasting antro-oral fistulas, the defects were healed. In 3 patients with 5 cases of tissue loss after osteoradionecrosis, we obtained healing of the wound in only 1 case. Histological examination of the cultures indicated that cultured cells formed well-differentiated layers, very similar to the keratinocytes of mucous membranes, although those seeded on amniotic membrane formed a single layer of cells, while those seeded on the PLLA scaffold were arranged on 2 or more layers: these differences were shown to be statistically significant with a morphometric analysis. Conclusions Autologous transplants of epithelium cultured on amniotic membrane and PLLA constitute a new and effective way of covering nonhealing tissue loss in the oral cavity in chosen cases, using modern methods of tissue engineering.

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