Accelerated Chemical Reactions for Lab-on-a-Chip Applications Using Electrowetting-Induced Droplet Self-Oscillations

2006 ◽  
Vol 915 ◽  
Author(s):  
Joanna Aizenberg ◽  
Tom Krupenkin ◽  
Paul Kolodner
Keyword(s):  
Chemical Reactions ◽  
Light Emission ◽  
Lab On A Chip ◽  
Experimental Investigations ◽  
Dna Oligomers ◽  
Complementary Dna ◽  
Rapid Mixing ◽  
Biochemical Assays ◽  
Dna Strands ◽  
Viable Method

AbstractThis paper summarizes the results of experimental investigations of the feasibility of applying electrowetting-induced droplet self-oscillations to induce rapid mixing of small quantities of liquids. The concept was tested using video microscopy to monitor the mixing of passive colored dyes, of spatially-separated reactants that change color upon reaction, and of fluorescent DNA oligomers whose light emission vanishes upon hybridization with appropriately-functionalized complementary DNA strands. Droplet self-oscillation was found to increase the rate of mixing by factors ranging from 15 to 100 as compared with the rate of passive diffusion in undisturbed droplets. This demonstrates that self-oscillation-induced mixing is a viable method for substantially enhancing the speed of chemical reactions in general, and biochemical assays in particular, when performed in small volumes of liquids.

Acyclic analogs of nucleosides based on tris(hydroxymethyl)phosphine oxide: synthesis and incorporation into short DNA oligomers

2015 ◽  
Vol 21 (5) ◽  
pp. 303-314 ◽  
Author(s):  
Barbara Nawrot ◽  
Olga Michalak ◽  
Barbara Mikołajczyk ◽  
Wojciech J. Stec
Keyword(s):  
Phosphine Oxide ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Hydroxyl Groups ◽  
Dna Oligomers ◽  
Phase Synthesis ◽  
Complementary Dna ◽  
Dna Analogs ◽  
Dna Strands ◽  
Synthesis Protocol

AbstractTris-(hydroxymethyl)phosphine oxide (THPO) to a certain extent resembles a part of 2′-deoxyribofuranose, although it exists in an acyclic form only and the oxygen atom at the THPO phosphorus center provides additional hydration site or acceptor of hydrogen bonds. After proper protection of hydroxyl groups, THPO was functionalized with nucleobases and converted into phosphoramidite monomers suitable for incorporation into growing oligonucleotide chains within the solid phase synthesis protocol. The resultant THPO-DNA analogs show reduced affinity to complementary DNA strands, and are resistant towards snake venom and calf spleen exonucleases.

scholarly journals Temperature Dependence of Unbinding Forces between Complementary DNA Strands

2002 ◽  
Vol 82 (1) ◽  
pp. 517-521 ◽  
Author(s):  
Irina Schumakovitch ◽  
Wilfried Grange ◽  
Torsten Strunz ◽  
Patricia Bertoncini ◽  
Hans-Joachim Güntherodt ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Complementary Dna ◽  
Dna Strands

scholarly journals Separation of 1–23-kb complementary DNA strands by urea–agarose gel electrophoresis

2009 ◽  
Vol 37 (17) ◽  
pp. e112-e112 ◽  
Author(s):  
Éva Hegedüs ◽  
Endre Kókai ◽  
Alexander Kotlyar ◽  
Viktor Dombrádi ◽  
Gábor Szabó
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Complementary Dna ◽  
Dna Strands

scholarly journals Why are complementary DNA strands symmetric?

2002 ◽  
Vol 18 (8) ◽  
pp. 1021-1033 ◽  
Author(s):  
P.-F. Baisnee ◽  
S. Hampson ◽  
P. Baldi
Keyword(s):  
Complementary Dna ◽  
Dna Strands

A Microfluidic Mixer Fabricated From Compliant Thermoplastic Films

2008 ◽  
Vol 2 (1) ◽  
Author(s):  
N. Paya ◽  
T. Dankovic ◽  
A. Feinerman
Keyword(s):  
Reynolds Number ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Reynolds Numbers ◽  
Mixing Efficiency ◽  
Minimal Amount ◽  
Microfluidic Mixer ◽  
Mixing Performance ◽  
Rapid Mixing ◽  
Microfluidic Flows

Mixing is often crucial to the operation of various microfluidic devices. And the most common objective is rapid mixing between two initially segregated fluid streams in a minimal amount of space. In microfluidic flows characterized by incompressibility and low Reynolds number, however, turbulence is almost entirely absent and mixing generally relies on diffusion. Therefore, based on the properties of the fluids involved, it can take impractically long to achieve high mixing efficiency in some cases. To resolve this problem, this paper demonstrates a novel compliant micromixer made of thermoplastic films for lab-on-a-chip applications. The microfluidic mixer utilizes self-rotation effects to achieve high mixing efficiency at Reynolds numbers below 100. In addition, a possible design is suggested for a thermoplastic voltage-actuated micromixer which can lead to even better mixing performance at Reynolds numbers below 1.

Base sequence- and T m-dependent DNA oligomer separation by open tubular capillary columns carrying complementary DNA oligomers as probes

2007 ◽  
Vol 388 (4) ◽  
pp. 919-928 ◽  
Author(s):  
Kamakshaiah Charyulu Devarayapalli ◽  
Seung Pil Pack ◽  
Nagendra Kumar Kamisetty ◽  
Mitsuru Nonogawa ◽  
Seiya Watanabe ◽  
...  
Keyword(s):  
Capillary Columns ◽  
Base Sequence ◽  
Dna Oligomers ◽  
Complementary Dna ◽  
Dna Oligomer Separation
Sign in / Sign up

Export Citation Format

Share Document