Label-free Electrochemical Impedance Detection of Ovarian Cancer Markers CA-125 and CEA

2009 ◽  
Vol 1236 ◽  
Author(s):  
Allison M Whited ◽  
Kanwar V Singh ◽  
Raj Solanki ◽  
David R Evans
Keyword(s):  
Ovarian Cancer ◽  
Electrochemical Impedance ◽  
Electrode Array ◽  
Ca 125 ◽  
Target Antigen ◽  
Label Free ◽  
Buffer Solution ◽  
Interdigitated Electrode ◽  
Binding Event ◽  
Interdigitated Electrode Array

AbstractCA-125 and carcinoembryonic antigen (CEA) are two biomarkers present in blood that can indicate the presence of ovarian cancer. They can also be used, both in conjunction with each other and independently, to determine the effectiveness of the treatment being meted for the disease. A label-free multiplexed interdigitated electrode array (IDEA) immunosensor was developed to detect both CA-125 and CEA in buffer solution at levels typically seen in patients with ovarian cancer . Electrochemical impedance spectroscopy was used to measure the increase in impedance when a binding event occurred between the target antigen and its specific antibody that was anchored to the surface of an interdigitated electrode array. CA-125 was detected in concentrations as low as 10units/mL and as high as 80units/mL. CEA was detected in concentrations as low as 1pg/mL and as high as 10μg/mL.

Three-dimensional interdigitated electrode array as a transducer for label-free biosensors

2008 ◽  
Vol 24 (4) ◽  
pp. 729-735 ◽  
Author(s):  
Andrey Bratov ◽  
Javier Ramón-Azcón ◽  
Natalia Abramova ◽  
Angel Merlos ◽  
Javier Adrian ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Electrode Array ◽  
Label Free ◽  
Interdigitated Electrode ◽  
Interdigitated Electrode Array

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

Boronate-Modified Interdigitated Electrode Array for Selective Impedance-Based Sensing of Glycated Hemoglobin

2016 ◽  
Vol 88 (19) ◽  
pp. 9582-9589 ◽  
Author(s):  
Yuwadee Boonyasit ◽  
Wanida Laiwattanapaisal ◽  
Orawon Chailapakul ◽  
Jenny Emnéus ◽  
Arto R. Heiskanen
Keyword(s):  
Glycated Hemoglobin ◽  
Electrode Array ◽  
Interdigitated Electrode ◽  
Interdigitated Electrode Array

Nanoparticle-Enhanced Sensitivity of a Nanogap-Interdigitated Electrode Array Impedimetric Biosensor

Langmuir ◽  
2011 ◽  
Vol 27 (22) ◽  
pp. 13931-13939 ◽  
Author(s):  
Kanwar V. Singh ◽  
Dheeraj K. Bhura ◽  
Gopichand Nandamuri ◽  
Allison M. Whited ◽  
David Evans ◽  
...  
Keyword(s):  
Electrode Array ◽  
Interdigitated Electrode ◽  
Enhanced Sensitivity ◽  
Impedimetric Biosensor ◽  
Interdigitated Electrode Array
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