Optically Driven Micromanipulation Tools Fabricated by Two-photon Microstereolithography

2002 ◽  
Vol 739 ◽  
Author(s):  
Shoji Maruo ◽  
Koji Ikuta ◽  
Hayato Korogi
Keyword(s):  
Laser Beam ◽  
Biological Samples ◽  
Living Cell ◽  
Optical Trapping ◽  
Cell Protein ◽  
Two Photon ◽  
Focal Position ◽  
Handling Method

ABSTRACTLight-driven micromanipulators have been developed by two-photon microstereolithography. The manipulators are driven and controlled by optical trapping. The torque of micromanipulator was successfully controlled on the order of femto-newton by adjusting the focal position of the trapped laser beam. Nanotweezers and a nanoneedle with probe tip of diameter 250 nm were fabricated and driven in a liquid. Such remote-controlled manipulation tools provide a unique and effective handling method of biological samples such as living cell, protein and DNA.

scholarly journals Micro-Dumbbells—A Versatile Tool for Optical Tweezers

2018 ◽  
Author(s):  
Weronika Lamperska ◽  
Sławomir Drobczyński ◽  
Michał Nawrot ◽  
Piotr Wasylczyk ◽  
Jan Masajada
Keyword(s):  
Laser Beam ◽  
Optical Tweezers ◽  
Optical Trapping ◽  
Viscosity Measurements ◽  
Two Photon ◽  
Vibration Detection ◽  
Holographic Optical Tweezers ◽  
Versatile Tool ◽  
Micro Tool

Manipulation of micro- and nano-sized objects with optical tweezers is a well established, albeit still evolving technique. While many objects can be trapped directly with focused laser beam(s), for some applications indirect manipulation with tweezers-operated tools is preferred. We introduce a simple, versatile micro-tool operated with holographic optical tweezers. The 40 µm long dumbbell-shaped tool, fabricated with two-photon laser 3D photolithography has two beads for efficient optical trapping and a probing spike on one end. We demonstrate fluids viscosity measurements and vibration detection as examples of possible applications.

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Observation of two-times self-focusing of femtosecond laser beam in ZnO crystal by two-photon luminescence

2018 ◽  
Vol 63 (21) ◽  
pp. 1392-1396 ◽  
Author(s):  
Xiaorui Wang ◽  
Honggang Ye ◽  
Zhicheng Su ◽  
Dapeng Yu ◽  
Shijie Xu
Keyword(s):  
Femtosecond Laser ◽  
Laser Beam ◽  
Two Photon ◽  
Self Focusing ◽  
Femtosecond Laser Beam ◽  
Zno Crystal

Effect of focal position on cut surface quality in laser cutting of 50-mm thick stainless steel

2021 ◽  
pp. 2140018
Author(s):  
Moo-Keun Song ◽  
Jong-Do Kim ◽  
Dong-Sig Shin ◽  
Su-Jin Lee ◽  
Dae-Won Cho
Keyword(s):  
Stainless Steel ◽  
Laser Beam ◽  
Laser Cutting ◽  
Nuclear Power ◽  
Surface Characteristics ◽  
Kerf Width ◽  
Power Structures ◽  
Thick Sample ◽  
Focal Position ◽  
Cut Surface

In this study, the parameters for underwater laser cutting of 50-mm thick stainless steel, which is typically used in nuclear power structures, are investigated. The focal position of laser beam significantly affects the cutting quality. In particular, in the cutting of the thick sample, change in the focal position determines the kerf width and the roughness of the cut surface. Moreover, the effects of the variation of kerf width and the cut surface characteristics on the focal position of the laser beam are investigated. As the focal position moved to the inside of the material, the upper kerf width increased, but the quality of the cut surface was improved.

Video-Rate Scanning Two-Photon Excitation Fluorescence Microscopy

1998 ◽  
Vol 4 (S2) ◽  
pp. 424-425
Author(s):  
G.Y. Fan ◽  
H. Fujisaki ◽  
R.-K. Tsay ◽  
R.Y. Tsien ◽  
Mark H. Ellisman
Keyword(s):  
Laser Beam ◽  
Group Velocity Dispersion ◽  
Laser System ◽  
Average Power ◽  
Laser Pulses ◽  
Green Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Schematic Layout ◽  
Two Photon Excitation

A video-rate scanning two-photon excitation microscope (TPEM) has been successfully constructed and tested. The TPEM, based on a Nikon RCM-8000, incorporates a femtosecond pulsed laser, a pre-chirper, and a non-confocal detection box for ratio imaging. Fig. 1 shows the schematic layout of the main components of the instrument, each of which is briefly discussed below.Laser System: A Tsunami Ti: Sapphire laser (from Spectra-Physics) is optically pumped by a 5 W green laser (Millennia from Spectra-Physics) and is capable of generating 100 fs pulses at a repetition rate of 82 MHz and an average power of 0.8 W. The output wavelength is tunable from 690 to 1050 nm with three optical sets, each covering part of the spectrum with some overlapping.Pre-chirper: After leaving the Tsunami, the laser beam enters an optic unit known as a pre-chirper which pre-chirps laser pulses to compensate for the group velocity dispersion which will result when the laser beam goes through the microscope optics.

Two-photon polymerisation of novel shapes using a conically diffracted femtosecond laser beam

2011 ◽  
Vol 284 (14) ◽  
pp. 3571-3574 ◽  
Author(s):  
C.F. Phelan ◽  
R.J. Winfield ◽  
D.P. O'Dwyer ◽  
Y.P. Rakovich ◽  
J.F. Donegan ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Beam ◽  
Two Photon ◽  
Femtosecond Laser Beam

A new water-soluble two-photon fluorescent probe for detection of trace benzoyl peroxide in wheat flour and in living cell and tissue imaging

2019 ◽  
Vol 43 (7) ◽  
pp. 3169-3173 ◽  
Author(s):  
Haiyuan Ding ◽  
Gangqiang Yuan ◽  
Liyi Zhou
Keyword(s):  
Reactive Oxygen Species ◽  
Human Health ◽  
Fluorescent Probe ◽  
Benzoyl Peroxide ◽  
Small Molecule ◽  
Living Cell ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Oxygen Species ◽  
Two Photon

Benzoyl peroxide (BPO), a member of small-molecule reactive oxygen species (ROS), has attracted considerable attention because of its impact on human health and industrial importance.

scholarly journals Boron Difluoride Curcuminoid Fluorophores with Enhanced Two-Photon Excited Fluorescence Emission and Versatile Living-Cell Imaging Properties

2016 ◽  
Vol 22 (15) ◽  
pp. 5219-5232 ◽  
Author(s):  
Kenji Kamada ◽  
Tomotaka Namikawa ◽  
Sébastien Senatore ◽  
Cédric Matthews ◽  
Pierre-François Lenne ◽  
...  
Keyword(s):  
Living Cell ◽  
Cell Imaging ◽  
Fluorescence Emission ◽  
Two Photon ◽  
Boron Difluoride ◽  
Living Cell Imaging ◽  
Imaging Properties
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