INHIBITORY EFFECTS OF SOME PESTICIDES AND METALS ON CARBONIC ANHYDRASE PURIFIED FROM SHABUT FISH (BARBUS GRYPUS) GILL TISSUE

In this study, a novel silver N-heterocyclic carbene (Ag-NHC) complex bearing hydroxyethyl substituent has been synthesized from the hydroxyethyl-substituted benzimidazolium salt and silver oxide by using in-situ deprotonation method. A structure of the Ag-NHC complex was characterized by using UV-Vis, FTIR, 1H-NMR and 13C-NMR spectroscopies and elemental analysis techniques. Also, the crystal structure of the novel complex was determined by single-crystal X-ray diffraction method. In this paper, compound 1 showed excellent inhibitory effects against some metabolic enzymes. This complex had Ki of 1.14 0.26 µM against human carbonic anhydrase I (hCA I), 1.88±0.20 µM against human carbonic anhydrase II (hCA I), and 10.75±2.47 µM against α-glycosidase, respectively. On the other hand, the Ki value was found as 25.32±3.76 µM against acetylcholinesterase (AChE) and 41.31±7.42 µM against butyrylcholinesterase (BChE), respectively. These results showed that the complex had drug potency against some diseases related to using metabolic enzymes.

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Localization of action of chlorothiazide in the nephron of the dog

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1959.196.6.1346 ◽

1959 ◽

Vol 196 (6) ◽

pp. 1346-1351 ◽

Cited By ~ 43

Author(s):

Richard H. Kessler ◽

Klaus Hierholzer ◽

Ruth S. Gurd ◽

Robert F. Pitts

Keyword(s):

Sodium Chloride ◽

Carbonic Anhydrase ◽

Ion Transport ◽

Distal Segment ◽

Proximal Segment ◽

Inhibitory Effects ◽

Stop Flow

Localization of the actions of chlorothiazide in dogs has been studied by the ‘stop-flow’ technique. The compound interfered with the reabsorption within the proximal segment of a portion of the filtered sodium chloride. In this activity it exhibited effects qualitatively similar to those of chlormerodrin, an organic mercurial diuretic. In addition, chlorothiazide diminished H+ and NH4+ excretion while augmenting K+ secretion within the distal segment. These are typical of the carbonic anhydrase inhibitory effects observed previously with acetazoleamide and presented in this study with dichlorphenamide. Probenecid blocked the proximal secretion of chlorothiazide but did not alter its actions on ion transport.

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Inhibitory effects of hypo-osmotic stress on extracellular carbonic anhydrase and photosynthetic efficiency of green alga Dunaliella salina possibly through reactive oxygen species formation

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2012.01.018 ◽

2012 ◽

Vol 54 ◽

pp. 43-48 ◽

Cited By ~ 24

Author(s):

Wenhua Liu ◽

Yao Ming ◽

Ping Li ◽

Zhongwen Huang

Keyword(s):

Reactive Oxygen Species ◽

Carbonic Anhydrase ◽

Osmotic Stress ◽

Green Alga ◽

Photosynthetic Efficiency ◽

Dunaliella Salina ◽

Oxygen Species ◽

Inhibitory Effects ◽

Reactive Oxygen Species Formation ◽

Species Formation

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Inhibitory effects of ammonia and urea on gill carbonic anhydrase enzyme activity of rainbow trout (Oncorhynchus mykiss)

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2004.03.009 ◽

2004 ◽

Vol 17 (3) ◽

pp. 125-128 ◽

Cited By ~ 13

Author(s):

Şükriye ArasHisar ◽

Olcay Hisar ◽

Telat Yanık ◽

Sıtkı M Aras

Keyword(s):

Enzyme Activity ◽

Rainbow Trout ◽

Carbonic Anhydrase ◽

Oncorhynchus Mykiss ◽

Inhibitory Effects ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Carbonic Anhydrase Enzyme

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Microwave assisted solvent-free mannich bases: synthesis, characterization and effects of these compounds on HCA I and HCA II isozymes

Macedonian Journal of Chemistry and Chemical Engineering ◽

10.20450/mjcce.2021.1968 ◽

2021 ◽

Vol 40 (1) ◽

pp. 43

Author(s):

Bülent Büyükkıdan ◽

Nurgün Büyükkıdan ◽

Metin Bülbül ◽

Melek Yılmaz ◽

Evren Derrun Arslanbay ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Esterase Activity ◽

Inhibitory Concentration ◽

Mannich Bases ◽

Solvent Free ◽

Inhibitory Effects ◽

Solvent Free Conditions ◽

Control Compound ◽

Esterase Activities

Mannich bases (2a–d) of aromatic amines were synthesized by using a conventional microwave technique under solvent-free conditions and characterized by IR and NMR (1H and 13C) and elemental analysis. The inhibitory effects of the synthesized Mannich bases were examined in vitro by using hydratase and esterase assays on carbonic anhydrase I and II isozymes (hCA, EC 4.2.1.1) purified from human erythrocyte cells. Acetazolamide was used as the control compound. The values of IC50, the half-maximum inhibitory concentration, were found for hydratase and esterase activities. Only two compounds (2b and 2d) exhibited poor hCA I and hCA II inhibition effects on esterase activity. In contrast, compounds 2a and 2c could be used as carbonic anhydrase activators as a result of the increased esterase activity of hCA I and hCA II isozymes.

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