scholarly journals Identification of Downy Mildew Resistance Loci in Sunflower Germplasm

2017 ◽  
Vol 9 (4) ◽  
pp. 515-519 ◽  
Author(s):  
Maryam MIRZAHOSEIN-TABRIZI
Keyword(s):  
Helianthus Annuus ◽  
Downy Mildew ◽  
Genetic Map ◽  
Inbred Line ◽  
Resistance Genes ◽  
Positive Control ◽  
Inbred Lines ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Selection Of

Downy mildew caused by Plasmopara halstedii is one of the most economically important fungal diseases on sunflower (Helianthus annuus). To date, several downy mildew resistance genes called Pl genes have been reported on sunflower genetic map. Previous findings have confirmed that Iranian sunflower germplasms are harbouring Pl resistance genes that may be used to control downy mildew. In the current study, there were investigated the Pl5 and Pl16 downy mildew resistance genes in 51 inbred lines of Iranian sunflower, using PCR-based method. Fifteen differential lines carrying Pl5 and Pl16 downy mildew resistance genes were used as positive control. DNAs from 51 sunflower inbred lines were used in PCR reactions using primer pair RS1008 and Hap3 previously reported to serve as tightly linked to Pl16 and P5 loci, respectively. The PCR results confirmed the presence of two Pl16 and Pl5 bands with the size of about 280 and 1,580 bp, respectively, in differential lines. The results indicated that 1 inbred line out of 51 was found to carry Pl5 gene and 10 lines were found to carry Pl16 gene across the studied Iranian sunflower genotypes. These findings may be used to assist breeders for conservation and selection of downy mildew resistant sunflower genotypes.

scholarly journals Селекция лука репчатого с устойчивостью к пероноспорозу

2019 ◽  
Author(s):  
G.F. Monakhos ◽  
S.G. Monakhos ◽  
R.R. Alizhanova
Keyword(s):  
Disease Resistance ◽  
Downy Mildew ◽  
Resistance Gene ◽  
Inbred Lines ◽  
F1 Hybrids ◽  
Downy Mildew Resistance ◽  
European Origin ◽  
Molecular Genotyping ◽  
Breeding Station ◽  
Selection Of

На Селекционной станции имени Н.Н. Тимофеева гибридизацией с донором устойчивости к пероноспорозу F1 Santero, беккроссом, инбридингом и отбором на инфекционном фоне с помощью молекулярного маркера DMR1 создано 15 линий лука репчатого гомозиготного по гену устойчивости Pd1. Оценка в однолетней культуре позволила выделить линии с массой луковиц 100120 г с высокой сохранностью, которые могут быть использованы в селекции F1 гибридов в качестве отцовских компонентов.Fifteen homozygous for Pd1 downy mildew resistance gene onion lines were developed by hybridization followed backcrossing F1 Santero of downy mildew resistant onion accession of European origin and a collection of onion inbred lines from LC Breeding station after N.N. Timofeev. The resistant to downy mildew, caused by Peronospora destructor (Berk.), plants were selected in segregated populations based on disease resistance test and molecular genotyping using DMR1marker. The lines with a mass of bulbs 100 120 g with high preservation, which can be used in the selection of F1 hybrids as paternal components.

Detection and integration of gene mapping of downy mildew resistance in maize inbred lines though linkage and association

Euphytica ◽  
2012 ◽  
Vol 187 (3) ◽  
pp. 369-379 ◽  
Author(s):  
Chalermpol Phumichai ◽  
Julapark Chunwongse ◽  
Sansern Jampatong ◽  
Pichet Grudloyma ◽  
Taweesak Pulam ◽  
...  
Keyword(s):  
Gene Mapping ◽  
Downy Mildew ◽  
Inbred Lines ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Maize Inbred Lines

QTL mapping for downy mildew resistance in cucumber inbred line WI7120 (PI 330628)

2016 ◽  
Vol 129 (8) ◽  
pp. 1493-1505 ◽  
Author(s):  
Yuhui Wang ◽  
Kyle VandenLangenberg ◽  
Todd C. Wehner ◽  
Peter A. G. Kraan ◽  
Jos Suelmann ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Downy Mildew ◽  
Inbred Line ◽  
Downy Mildew Resistance ◽  
Mildew Resistance

scholarly journals SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance

2018 ◽  
Vol 13 (1) ◽  
pp. 319-326
Author(s):  
Ezgi Çabuk Şahin ◽  
Aral Kalenderoğlu ◽  
Yıldız Aydın ◽  
Göksel Evci ◽  
Ahu Altınkut Uncuoğlu
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Downy Mildew Resistance ◽  
Phenotypic Analysis ◽  
Breeding Programs ◽  
Mildew Resistance ◽  
Ssr Analysis ◽  
Genotypic Analysis ◽  
Simple Sequence

AbstractThe effectiveness of Pl genes is known to be resistant to downy mildew (DM) disease affected by fungus Plasmopara halstedii in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes Plarg, Pl13 and Pl8. A total of 69 simple sequence repeat markers and 241 F2 individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (Plarg, Pl8 and Pl13) and downy mildew inoculation tests were evaluated together and ORS716 (for Plarg and Pl13), HA4011 (for Pl8) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.

scholarly journals Mapping of easy to screen SSR markers for selection of RFLP markers-bracketed downy mildew resistance QTLs in pearl millet

2017 ◽  
Vol 151 (2) ◽  
pp. 401-411 ◽  
Author(s):  
Jyoti Taunk ◽  
Deepmala Sehgal ◽  
Neelam R. Yadav ◽  
Catherine Howarth ◽  
Ram C. Yadav ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Ssr Markers ◽  
Rflp Markers ◽  
Downy Mildew Resistance ◽  
Resistance Qtls ◽  
Mildew Resistance ◽  
Selection Of
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