scholarly journals Purification and characterization of a protease from Aspergillus sydowii URM5774: Coffee ground residue for protease production by solid state fermentation

2021 ◽  
Vol 93 (suppl 3) ◽  
Author(s):  
FELYPE T.B. ROCHA ◽  
ROMERO M.P. BRANDÃO-COSTA ◽  
ANNA GABRIELLY D. NEVES ◽  
KETHYLEN B.B. CARDOSO ◽  
THIAGO P. NASCIMENTO ◽  
...  
BioResources ◽  
2013 ◽  
Vol 8 (4) ◽  
Author(s):  
Jorge Álvarez-Cervantes ◽  
Edna Maria Hernández-Domínguez ◽  
Ainhoa Arana-Cuenca ◽  
Gerardo Díaz-Godínez ◽  
Yuridia Mercado-Flores

2020 ◽  
Vol 142 ◽  
pp. 104106 ◽  
Author(s):  
Fatma Kaaniche ◽  
Abdelaaty Hamed ◽  
Lobna Elleuch ◽  
Ahlem Chakchouk-Mtibaa ◽  
Slim Smaoui ◽  
...  

Engevista ◽  
2012 ◽  
Vol 15 (1) ◽  
pp. 61 ◽  
Author(s):  
Salah Din Mahmud Hasan ◽  
Citieli Giongo ◽  
Sonia Aparecida Reis Lopes-Shikida ◽  
Sérgio Luiz de Lucena ◽  
Mônica Lady Fiorese

The operation parameters used in solid-state fermentation (SSF) support the growth of filamentous fungi, which grow on solid substrates producing important metabolites such as proteases. The aim of this work is to obtain fungal proteases by SSF using the residues of a local brewery industry (barley bagasse and trub), that have high contents of proteins and soluble matter such as carbohydrates, vitamins, and mineral salts. The methodology includes the preparation of the residues, the screening of microorganisms, evaluation of the operation conditions for SSF using factorial design, purification and partial characterization of the protease. The results indicate that A. niger sp. UPT-03 isolated from the residue shows higher yields in terms of enzyme production (0.36 U gdm-1 h-1). The purification with DEAE-cellulose resulted in protease recovery with 30-fold of purification with a specific activity of 550 U mg protein-1. Higher proteolytic activity of purified enzyme was obtained at pH 5.5 and 55 ºC.


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