scholarly journals Optimisation of a quantitative polymerase chain reaction-based strategy for the detection and quantification of human herpesvirus 6 DNA in patients undergoing allogeneic haematopoietic stem cell transplantation

2015 ◽  
Vol 110 (4) ◽  
pp. 461-467 ◽  
Author(s):  
Miriam YH Ueda ◽  
Paulo G Alvarenga ◽  
Juliana M Real ◽  
Eloisa de Sá Moreira ◽  
Aripuanã Watanabe ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Haematopoietic Stem Cell Transplantation ◽  
Quantitative Polymerase Chain Reaction ◽  
Human Herpesvirus ◽  
Haematopoietic Stem Cell ◽  
Human Herpesvirus 6 ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Detection And Quantification

Human Herpesvirus 6 Reactivation Evaluated by Digital Polymerase Chain Reaction and Its Association With Dynamics of CD134-Positive T Cells After Allogeneic Hematopoietic Stem Cell Transplantation

2019 ◽  
Vol 220 (6) ◽  
pp. 1001-1007 ◽  
Author(s):  
Hitomi Nakayama ◽  
Rie Yamazaki ◽  
Jun Kato ◽  
Yuya Koda ◽  
Masatoshi Sakurai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
T Cells ◽  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Chain Reaction ◽  
Hematopoietic Stem ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

AbstractBackgroundHuman herpesvirus 6 (HHV-6) causes life-threatening central nervous system disorders after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent studies implicated CD134 as a specific receptor of HHV-6B and demonstrated that its expression levels in CD4-positive T cells after allo-HSCT could be related to the reactivation of HHV-6. We prospectively evaluated the relationship between HHV-6 reactivation and CD134+ T cells in the recipients of allo-HSCT.MethodsHHV-6 viral load in plasma was quantitatively measured weekly after allo-HSCT by digital polymerase chain reaction in 34 patients. The ratio of CD134 in CD4+ T cells (CD134/CD4 ratio) was serially measured by flow cytometry before and after transplantation.ResultsHHV-6 reactivation was detected in 23 patients (68%). The CD134/CD4 ratio before conditioning was significantly higher in patients with HHV-6 reactivation than in those without (median, 3.8% vs 1.5%, P < .01). In multivariate analysis, a higher CD134/CD4 ratio before conditioning was significantly associated with the incidence of HHV-6 reactivation (odds ratio, 10.5 [95% confidence interval, 1.3–85.1], P = .03).ConclusionsA higher CD134/CD4 ratio before conditioning was associated with a higher risk of HHV-6 reactivation, suggesting that the rate may be a promising marker for predicting HHV-6 reactivation after allo-HSCT.

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