Rearing two fruit flies pests on artificial diet with variable pH

Abstract Fruit flies (Diptera: Tephritidae) are considered the main fruit pests worldwide. In Brazil, two species are predominant: the South American fruit fly, Anastrepha fraterculus and the Mediterranean fruit fly, Ceratitis capitata. In this study, we evaluated the effect of artificial diets with variable pH in their larval development and adult performance. The experiments were carried out in the laboratory at 25 ± 2 °C, 70 ± 10% RH and 12:12h (L:D) photoperiod. Semisolid diets with pH values of 6.0, 5.0, 4.0, 3.0, 2.0, 1.5, and 1.0, adjusted by adding hydrochloric acid were tested. Results indicated that the diet with pH 6.0 did not support larval development of both species of fruit fly. Diets with greater acidic pH values did not allow egg, larvae or pupae development and adult reproduction of A. fraterculus. For C. capitata , the pH of artificial diet exerts greater influence compared to A. fraterculus on the duration and viability of the larval stage, number of pupae, sex ratio and longevity of males.

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Determining the Sterilization Doses under Hypoxia for the Novel Black Pupae Genetic Sexing Strain of Anastrepha fraterculus (Diptera, Tephritidae)

Insects ◽

10.3390/insects12040308 ◽

2021 ◽

Vol 12 (4) ◽

pp. 308

Author(s):

Paloma Della Giustina ◽

Thiago Mastrangelo ◽

Sohel Ahmad ◽

Gabriel Mascarin ◽

Carlos Caceres

Keyword(s):

Fruit Fly ◽

South American ◽

Response Curves ◽

Genetic Sexing ◽

Egg Hatch ◽

Anastrepha Fraterculus ◽

Common Strategy ◽

Genetic Sexing Strain ◽

South American Fruit Fly ◽

First Time

A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose–response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.

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Toxicity and residual effects of toxic baits with spinosyns on the South American fruit fly

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2018000200002 ◽

2018 ◽

Vol 53 (2) ◽

pp. 144-151 ◽

Cited By ~ 5

Author(s):

Inana Xavier Schutze ◽

Cléber Antonio Baronio ◽

Morgana Mattiello Baldin ◽

Alci Enimar Loek ◽

Marcos Botton

Keyword(s):

Residual Effect ◽

Fruit Fly ◽

Residual Effects ◽

South American ◽

Sugarcane Molasses ◽

Anastrepha Fraterculus ◽

Lethal Time ◽

The One ◽

Food Lures ◽

South American Fruit Fly

Abstract: The objective of this work was to assess the lethal concentration and lethal time (LC and LT) of spinosad and spinetoram, combined with different food lures, and their residual effects on South American fruit fly (Anastrepha fraterculus). The toxic baits were offered in eight concentrations (2, 6, 14, 35, 84, 204, 495, and 1,200 mg L-1), combined with the following food lures: 7% sugarcane molasses, 3% Biofruit, 1.5% CeraTrap, 1.25% Flyral, 3% Samaritá Bait, and 3% Samaritá Tradicional; diluted food lures in water were used as controls. The residual effect of the formulations at 96 mg L-1 concentration were evaluated for 21 days and were compared with that of the commercial bait Success 0.02 CB. Both insecticides were toxic to adults of A. fraterculus, and mortality varied with the food lure used. The LC50and LT50ranged from 15.19 to 318.86 mg L-1and from 11.43 to 85.93 hours, respectively. Spinosad was 2 to 36 times as toxic as spinetoram when combined with different hydrolyzed proteins. Toxic baits formulated with spinosad and spinetoram (96 mg L-1) caused mortality equivalent to the one by Success 0.02 CB (90.2%), when assessed on the day of application. Toxic baits formulated with 3% Biofruit + spinosad and 3% Samaritá Bait + spinetoram are effective for managingA. fraterculusand provide up to seven days of residual effect in the absence of rain; however, only Success 0.02 CB caused more than 80% mortality for up to 21 days.

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Reproductive behaviour of the South American fruit fly Anastrepha fraterculus (Diptera: Tephritidae): laboratory and field studies

Physiological Entomology ◽

10.1111/j.1365-3032.1994.tb01052.x ◽

1994 ◽

Vol 19 (4) ◽

pp. 271-277 ◽

Cited By ~ 20

Author(s):

I. S. DE LIMA ◽

P. E. HOWSE ◽

L. A. B. SALLES

Keyword(s):

Fruit Fly ◽

Field Studies ◽

Reproductive Behaviour ◽

South American ◽

The South ◽

Anastrepha Fraterculus ◽

South American Fruit Fly

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Comparison of Glass and Plastic McPhail Traps in the Capture of the South American Fruit Fly, Anastrepha fraterculus (Diptera: Tephritidae) in Brazil

Florida Entomologist ◽

10.2307/3494843 ◽

1991 ◽

Vol 74 (3) ◽

pp. 467 ◽

Cited By ~ 5

Author(s):

Mirna Duarte Barros ◽

Pérsio Marcondes do Amaral ◽

Aldo Malavasi ◽

Persio Marcondes do Amaral

Keyword(s):

Fruit Fly ◽

South American ◽

The South ◽

Anastrepha Fraterculus ◽

South American Fruit Fly

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Remating behavior in Anastrepha fraterculus (Diptera: Tephritidae) females is affected by male juvenile hormone analog treatment but not by male sterilization

Bulletin of Entomological Research ◽

10.1017/s0007485312000727 ◽

2013 ◽

Vol 103 (3) ◽

pp. 310-317 ◽

Cited By ~ 26

Author(s):

S. Abraham ◽

M.C. Liendo ◽

F. Devescovi ◽

P.A. Peralta ◽

V. Yusef ◽

...

Keyword(s):

Juvenile Hormone ◽

Protein Intake ◽

Fruit Fly ◽

South American ◽

Field Cage ◽

Anastrepha Fraterculus ◽

Hormone Analog ◽

Juvenile Hormone Mimic ◽

Sperm Volume ◽

South American Fruit Fly

AbstractThe sterile insect technique (SIT) has been proposed as an area-wide method to control the South American fruit fly, Anastrepha fraterculus (Wiedemann). This technique requires sterilization, a procedure that affects, along with other factors, the ability of males to modulate female sexual receptivity after copulation. Numerous pre-release treatments have been proposed to counteract the detrimental effects of irradiation, rearing and handling and increase SIT effectiveness. These include treating newly emerged males with a juvenile hormone mimic (methoprene) or supplying protein to the male's diet to accelerate sexual maturation prior to release. Here, we examine how male irradiation, methoprene treatment and protein intake affect remating behavior and the amount of sperm stored in inseminated females. In field cage experiments, we found that irradiated laboratory males were equally able to modulate female remating behavior as fertile wild males. However, females mated with 6-day-old, methoprene-treated males remated more and sooner than females mated with naturally matured males, either sterile or wild. Protein intake by males was not sufficient to overcome reduced ability of methoprene-treated males to induce refractory periods in females as lengthy as those induced by wild and naturally matured males. The amount of sperm stored by females was not affected by male irradiation, methoprene treatment or protein intake. This finding revealed that factors in addition to sperm volume intervene in regulating female receptivity after copulation. Implications for SIT are discussed.

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