scholarly journals Pathogenic potential of Brucella ovis field isolates with different genotypic profile and protection provided by the vaccine strain B. ovis ΔabcBA against B. ovis field isolates in mice

2020 ◽  
Vol 40 (2) ◽  
pp. 88-96 ◽  
Author(s):  
Thaynara P. Carvalho ◽  
Noelly Q. Ribeiro ◽  
Juliana P.S. Mol ◽  
Fabíola B. Costa ◽  
Camila Eckstein ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Reference Strain ◽  
In Vitro Growth ◽  
Initial Screening ◽  
Pathogenic Potential ◽  
Field Isolates ◽  
Brucella Ovis ◽  
Reproductive Losses ◽  
Genotypic Profile

ABSTRACT: Brucella ovis causes economic and reproductive losses in sheep herds. The goal of this study was to characterize infection with B. ovis field isolates in a murine model, and to evaluate protection induced by the candidate vaccine strain B. ovis ΔabcBA in mice challenged with these field isolates. B. ovis field strains were able to colonize and cause lesions in the liver and spleen of infected mice. After an initial screening, two strains were selected for further characterization (B. ovis 94 AV and B. ovis 266 L). Both strains had in vitro growth kinetics that was similar to that of the reference strain B. ovis ATCC 25840. Vaccination with B. ovis ΔabcBA encapsulated with 1% alginate was protective against the challenge with field strains, with the following protection indexes: 0.751, 1.736, and 2.746, for mice challenged with B. ovis ATCC25840, B. ovis 94 AV, and B. ovis 266 L, respectively. In conclusion, these results demonstrated that B. ovis field strains were capable of infecting and inducing lesions in experimentally infected mice. The attenuated vaccine strain B. ovis ΔabcBA induced protection in mice challenged with different B. ovis field isolates, resulting in higher protection indexes against more pathogenic strains.

scholarly journals Evaluation of Staphylococcal Activity of Garcinia kola Almonds

2019 ◽  
pp. 1-7
Author(s):  
Ouattara Karamoko ◽  
Dibi Koffi Saint Didier ◽  
Kone Monon ◽  
Ouattara Abou ◽  
Bagre Issa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Reference Strain ◽  
Susceptibility Testing ◽  
Traditional Society ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Cost Of Treatment ◽  
Garcinia Kola

The emergence of infectious diseases, particularly staphylococcal infections, treatment failures and the more high cost of treatment of infections caused by resistant staphylococci called to find other care alternatives. This study was initiated to evaluate the antibacterial activity of the aqueous extract from Garcinia kola almonds on the in vitro growth of Staphylococcus aureus strains. The methods of diffusion in agar and liquid media were used for susceptibility testing and MIC and MBC determination. The tests were performed on four strains of S. aureus and one reference strain. The minimum inhibitory concentrations of the extracts ranged from 3.12 mg/mL and 12.5 mg/mL and the minimum bactericidal concentrations between 6.25 mg/mL and 25 mg/mL. The lowest value of MIC and MBC was observed with S. aureus ATCC 29213 while the greatest value of these same parameters was obtained on S. aureus 993C/18 and S. aureus 1075C/18. The aqueous almonds extract of Garcinia kola had a bactericidal activity on all the strains of S. aureus studied. This could justify the use of Garcinia kola almonds in the treatment of various diseases in traditional society.

ANTIBODY-MEDIATED IN VITRO GROWTH INHIBITION OF FIELD ISOLATES OF PLASMODIUM FALCIPARUM FROM ASYMPTOMATIC CHILDREN IN BURKINA FASO

2003 ◽  
Vol 68 (6) ◽  
pp. 728-733 ◽  
Author(s):  
AHMED BOLAD ◽  
ALFRED TRAORE ◽  
KLAVS BERZINS ◽  
NADINE CUZIN-OUATTARA ◽  
ISSA NEBIÉ ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Burkina Faso ◽  
In Vitro Growth ◽  
Field Isolates ◽  
Asymptomatic Children

Inhibition of in vitro growth of Plasmodium falciparum field isolates mediated by human antibodies to Pf155/RESA and Pf332

1999 ◽  
Vol 21 (6) ◽  
pp. 331-334 ◽  
Author(s):  
BIRGITTA WAHLIN FLYG ◽  
ABU BAKAR SIDDIQUE ◽  
PETER PERLMANN ◽  
FULVIO ESPOSITO ◽  
KLAVS BERZINS
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Growth ◽  
Human Antibodies ◽  
Field Isolates

Effect of in Vivo Oxidized Cellulose on in Vitro Growth of Human Respiratory Mucosa and Sub-mucosa During Endoscopic Skull Base Approaches

2016 ◽  
Vol 77 (S 01) ◽  
Author(s):  
Ezequiel Goldschmidt ◽  
Jorge Rasmussen ◽  
Joseph Chabot ◽  
Monica Loressi ◽  
Marcelo Ielpi ◽  
...  
Keyword(s):  
Skull Base ◽  
Oxidized Cellulose ◽  
In Vitro Growth ◽  
Respiratory Mucosa

scholarly journals Sero- and apx-typing of German Actinobacillus pleuropneumoniae field isolates from 2010 to 2019 reveals a predominance of serovar 2 with regular apx-profile

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Lukas Schuwerk ◽  
Doris Hoeltig ◽  
Karl-Heinz Waldmann ◽  
Peter Valentin-Weigand ◽  
Judith Rohde
Keyword(s):  
Reference Strain ◽  
Actinobacillus Pleuropneumoniae ◽  
Etiological Agent ◽  
Toxin Gene ◽  
Gene Profile ◽  
Field Isolates ◽  
Porcine Pleuropneumonia ◽  
Virulent Strains ◽  
Highly Virulent

AbstractSerotyping is the most common method to characterize field isolates of Actinobacillus (A.) pleuropneumoniae, the etiological agent of porcine pleuropneumonia. Based on serology, many farms seem to be infected and antibodies against a wide variety of serovars are detectable, but, so far it is unknown to what degree respective serovars contribute to outbreaks of clinical manifest disease. In this study, 213 German A. pleuropneumoniae field isolates retrieved for diagnostic purposes from outbreaks of porcine pleuropneumonia between 2010 and 2019 were genetically serotyped and analyzed regarding their apx-toxin gene profile using molecular methods. Serotyping revealed a prominent role of serovar 2 in clinical cases (64% of all isolates) and an increase in the detection of this serovar since 2010 in German isolates. Serovar 9/11 followed as the second most frequent serovar with about 15% of the isolates. Furthermore, very recently described serovars 16 (n = 2) and 18 (n = 8) were detected. Most isolates (93.4%) showed apx-profiles typical for the respective serovar. However, this does not hold true for isolates of serovar 18, as 75% (n = 6) of all isolates of this serovar deviated uniformly from the “typical” apx-gene profile of the reference strain 7311555. Notably, isolates from systemic lesions such as joints or meninges did not harbor the complete apxICABD operon which is considered typical for highly virulent strains. Furthermore, the extremely low occurrence (n = 1) of NAD independent (biovar II) isolates in German A. pleuropneumoniae was evident in our collection of clinical isolates.

scholarly journals Kenyan Plasmodium falciparum Field Isolates: Correlation between Pyrimethamine and Chlorcycloguanil Activity In Vitro and Point Mutations in the Dihydrofolate Reductase Domain

1998 ◽  
Vol 42 (1) ◽  
pp. 164-169 ◽  
Author(s):  
A. Nzila-Mounda ◽  
E. K. Mberu ◽  
C. H. Sibley ◽  
C. V. Plowe ◽  
P. A. Winstanley ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Dihydrofolate Reductase ◽  
Drug Response ◽  
Point Mutations ◽  
Distinct Group ◽  
Wild Type ◽  
Field Isolates ◽  
Vitro Data ◽  
In Vitro Data

ABSTRACT Sixty-nine Kenyan Plasmodium falciparum field isolates were tested in vitro against pyrimethamine (PM), chlorcycloguanil (CCG), sulfadoxine (SD), and dapsone (DDS), and their dihydrofolate reductase (DHFR) genotypes were determined. The in vitro data show that CCG is more potent than PM and that DDS is more potent than SD. DHFR genotype is correlated with PM and CCG drug response. Isolates can be classified into three distinct groups based on their 50% inhibitory concentrations (IC50s) for PM and CCG (P< 0.01) and their DHFR genotypes. The first group consists of wild-type isolates with mean PM and CCG IC50s of 3.71 ± 6.94 and 0.24 ± 0.21 nM, respectively. The second group includes parasites which all have mutations at codon 108 alone or also at codons 51 or 59 and represents one homogeneous group for which 25- and 6-fold increases in PM and CCG IC50s, respectively, are observed. Parasites with mutations at codons 108, 51, and 59 (triple mutants) form a third distinct group for which nine- and eightfold increases in IC50s, respectively, of PM and CCG compared to the second group are observed. Surprisingly, there is a significant decrease (P < 0.01) of SD and DDS susceptibility in these triple mutants. Our data show that more than 92% of Kenyan field isolates have undergone at least one point mutation associated with a decrease in PM activity. These findings are of great concern because they may indicate imminent PM-SD failure, and there is no affordable antimalarial drug to replace PM-SD (Fansidar).

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