scholarly journals Cloning and expression of Neospora caninum rhoptry 2 and its potential as an immunobiological for neosporosis control

2021 ◽  
Vol 41 ◽  
Author(s):  
Alceu G. dos Santos Junior ◽  
Neida Lucia Conrad ◽  
Renan Eugênio A. Piraine ◽  
Rodrigo C. Cunha ◽  
Renato Andreotti ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Parasitophorous Vacuole ◽  
Cloning And Expression ◽  
Igg Antibodies ◽  
Subunit Vaccines ◽  
Expression In Escherichia Coli ◽  
Insoluble Form ◽  
Specific Igg ◽  
Specific Igg Antibodies ◽  
Antigenic Epitopes

ABSTRACT: The protozoan Neospora caninum is known worldwide as one of the main causes of abortion in cattle. During infection, rhoptry proteins present in the apical complex of the parasite play important roles in adhesion and parasitophorous vacuole formation. The use of N. caninum ROP2 in experimental vaccines has shown promising protective results. In our study we performed cloning and expression in Escherichia coli of an antigenic portion of N. caninum ROP2. The recombinant protein (rROP2) was obtained in insoluble form, and the purified protein showed a size of approximately 18kDa. Even being a small truncate NcROP2 region, it was possible to conserve the antigenic epitopes which were recognized by bovine serum naturally infected with N. caninum. Vaccination with rROP2 on aluminum hydroxide adjuvant induced high levels of rROP2-specific IgG antibodies capable of recognizing native protein in tachyzoite lysates. In conclusion, our approaches were effective in obtaining the rROP2 protein, which induced specific mouse immune response and was also recognized by sera from N. caninum naturally infected cattle. These results suggest that it is a promising antigen for the development of neosporosis subunit vaccines as well as a suitable antigen for use in immunodiagnosis.

scholarly journals Endogenously Produced SARS-CoV-2 Specific IgG Antibodies May Have a Limited Impact on Clearing Nasal Shedding of Virus during Primary Infection in Humans

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 516
Author(s):  
Shuyi Yang ◽  
Keith R. Jerome ◽  
Alexander L. Greninger ◽  
Joshua T. Schiffer ◽  
Ashish Goyal
Keyword(s):  
Production Rate ◽  
Neutralizing Antibodies ◽  
Primary Infection ◽  
Natural Infection ◽  
Viral Clearance ◽  
Clinical Severity ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
Specific Igg ◽  
Specific Igg Antibodies

While SARS-CoV-2 specific neutralizing antibodies have been developed for therapeutic purposes, the specific viral triggers that drive the generation of SARS-CoV-2 specific IgG and IgM antibodies remain only partially characterized. Moreover, it is unknown whether endogenously derived antibodies drive viral clearance that might result in mitigation of clinical severity during natural infection. We developed a series of non-linear mathematical models to investigate whether SARS-CoV-2 viral and antibody kinetics are coupled or governed by separate processes. Patients with severe disease had a higher production rate of IgG but not IgM antibodies. Maximal levels of both isotypes were governed by their production rate rather than different saturation levels between people. Our results suggest that an exponential surge in IgG levels occurs approximately 5–10 days after symptom onset with no requirement for continual antigenic stimulation. SARS-CoV-2 specific IgG antibodies appear to have limited to no effect on viral dynamics but may enhance viral clearance late during primary infection resulting from the binding effect of antibody to virus, rather than neutralization. In conclusion, SARS-CoV-2 specific IgG antibodies may play only a limited role in clearing infection from the nasal passages despite providing long-term immunity against infection following vaccination or prior infection.

P161 Kidney allograft outcomes associated with locus specific IgG antibodies to donor class I

2017 ◽  
Vol 78 ◽  
pp. 175
Author(s):  
Rex Friedlander ◽  
Essa Abuhelaiqa ◽  
Prabhakar Putheti ◽  
Arvind K. Menon ◽  
Vijay K. Sharma ◽  
...  
Keyword(s):  
Class I ◽  
Igg Antibodies ◽  
Kidney Allograft ◽  
Specific Igg ◽  
Specific Igg Antibodies

scholarly journals Neospora caninuminfection in birds: experimental infections in chicken and embryonated eggs

Parasitology ◽  
2007 ◽  
Vol 134 (14) ◽  
pp. 1931-1939 ◽  
Author(s):  
P. I. FURUTA ◽  
T. W. P. MINEO ◽  
A. O. T. CARRASCO ◽  
G. S. GODOY ◽  
A. A. PINTO ◽  
...  
Keyword(s):  
Laying Hens ◽  
Neospora Caninum ◽  
Parasite Burden ◽  
Asymptomatic Infection ◽  
Igg Antibodies ◽  
Intermediate Hosts ◽  
Susceptibility To Infection ◽  
Experimental Infections ◽  
Inoculum Dose ◽  
Specific Igg

SUMMARYNeospora caninumcauses economical impact in cattle-raising farms since it is implicated as the major cause of bovine abortions. Although infection by the parasite has been widely described in mammals, the role of birds in its life-cycle is still obscure. Therefore, this work aimed to evaluate the infection byN. caninumin different chicken models. Experimental infections were conducted in 7-day-old chicks, laying hens and embryonated eggs, where samples were analysed for parasite burden, IgG antibodies and lesions promoted. Chickens demonstrated an asymptomatic infection, although with seroconversion and systemic replication of the parasite. In laying hens, no signs of vertical transmission were observed. However, embryonated eggs inoculated by the allantoic cavity route demonstrated susceptibility to infection, with mortality rates around 50% independent of the inoculum dose. Additionally, dogs became infected after ingestion of different amounts of inoculated eggs, producing either oocysts or specific IgG antibodies. The results herein presented demonstrate that chickens may be intermediate hosts ofN. caninumand that embryonated eggs could be a useful model to study the parasite's biology.

scholarly journals Total serum IgE and parasite: specific IgG and IgA antibodies in human strongyloidiasis

1993 ◽  
Vol 35 (4) ◽  
pp. 361-365 ◽  
Author(s):  
Cláudio L. Rossi ◽  
Emilia E. H. Takahashi ◽  
Cláudia D. Partel ◽  
Lívia G.V.L. Teodoro ◽  
Luiz J. da Silva
Keyword(s):  
Elevated Serum ◽  
Clinical Manifestations ◽  
Case Series ◽  
Total Serum ◽  
Igg Antibodies ◽  
Serum Ige ◽  
Iga Antibodies ◽  
Specific Igg ◽  
Mean Concentration ◽  
Specific Igg Antibodies

Total serum IgE, and Strongyloides - specific IgG and IgA antibodies were studied in 27 patients with parasitologically proven strongyloidiasis. Clinical manifestations in this case series were investigated by a restrospective study of the patient's records. Total serum IgE levels were elevated (greater than 250 IU/ml) in 59% of the patients (mean concentration = 1364 IU/ml). Parasite - specific IgG and IgA antibodies were detected by ELISA in the serum of 23 (85.2%) and 21 (77.8%) patients, respectively. Elevated serum IgE and clinical manifestations were not useful indexes of the presence of strongyloidiasis. On the other hand, our results support the view that serologic tests, particularly ELISA for detecting Strongyloides - specific IgG antibodies, can be usefully exploited for diagnostic purposes in strongyloidiasis.

scholarly journals Antibodies to Protein but Not Glycolipid Structures Are Important for Host Defense againstMycoplasma pneumoniae

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Patrick M. Meyer Sauteur ◽  
Adrianus C. J. M. de Bruijn ◽  
Catarina Graça ◽  
Anne P. Tio-Gillen ◽  
Silvia C. Estevão ◽  
...  
Keyword(s):  
Neurological Disorders ◽  
Antibody Formation ◽  
Antibody Responses ◽  
Igg Antibodies ◽  
Wild Type ◽  
Content Type ◽  
Bruton Tyrosine Kinase ◽  
Specific Igg ◽  
Deficient Mice ◽  
Specific Igg Antibodies

ABSTRACTAntibody responses toMycoplasma pneumoniaecorrelate with pulmonaryM. pneumoniaeclearance. However,M. pneumoniae-specific IgG antibodies can cross-react with the myelin glycolipid galactocerebroside (GalC) and cause neurological disorders. We assessed whether antiglycolipid antibody formation is part of the physiological immune response toM. pneumoniae. We show that antibodies againstM. pneumoniaeproteins and glycolipids arise in serum ofM. pneumoniae-infected children and mice. Although antibodies toM. pneumoniaeglycolipids were mainly IgG, anti-GalC antibodies were only IgM. B-1a cells, shown to aid in protection against pathogen-derived glycolipids, are lacking in Bruton tyrosine kinase (Btk)-deficient mice.M. pneumoniae-infected Btk-deficient mice developedM. pneumoniae-specific IgG responses toM. pneumoniaeproteins but not toM. pneumoniaeglycolipids, including GalC. The equal recovery fromM. pneumoniaeinfection in Btk-deficient and wild-type mice suggests that pulmonaryM. pneumoniaeclearance is predominantly mediated by IgG reactive withM. pneumoniaeproteins and thatM. pneumoniaeglycolipid-specific IgG or IgM is not essential. These data will guide the development ofM. pneumoniae-targeting vaccines that avoid the induction of neurotoxic antibodies.

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