scholarly journals Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021 ◽  
Vol 28 (3) ◽  
Author(s):  
Rosa Armijos-González ◽  
Luisa Espinosa-Delgado ◽  
Augusta Cueva-Agila
Keyword(s):  
Shoot Regeneration ◽  
Somaclonal Variations

scholarly journals Three step protocol for regeneration of plantlets in Indian garlic varieties using root meristem

2018 ◽  
Author(s):  
Ashwini P. Benke ◽  
Punam Shelke ◽  
V. Mahajan
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Root Meristem ◽  
Normal Growth ◽  
Chemical Mutagen ◽  
Somaclonal Variations ◽  
Callus Proliferation

Protocol for plantlets regeneration through callus induction using root meristem was developed in present investigation. Different mediums were standardized for each step vis-a-via callus induction, callus proliferation and shoot regeneration. In first stage, B5 medium added with 0.7mg/l 2, 4-D and 1mg/l BAP showed 100% callusing while reduction in both growth regulators up to level of 0.5 mg/l 2,4-D and 0.1mg/l BAP results in significant callus proliferation. In last step, shoot regeneration was found to good and healthier in B5 medium supplemented with 0.1 mg/l 2, 4-D only. After regeneration normal growth in plantlets observed under plain B-5 medium, however no abnormality has been noted in developed population. Present protocol will be efficiently useful for chemical mutagen induction studies through callus, observing somaclonal variations and transgenic studies.

TDz Induced Multiple Shoot Regeneration and in Vitro Flowering From Nodal Explant in Withania Somnifera (L.)

2012 ◽  
Vol 3 (4) ◽  
pp. 50-52
Author(s):  
Nishesh Sharma ◽  
◽  
Manjul Dhiman ◽  
Eapen P Koshy
Keyword(s):  
Shoot Regeneration ◽  
Withania Somnifera ◽  
Multiple Shoot ◽  
In Vitro Flowering ◽  
Nodal Explant ◽  
Multiple Shoot Regeneration

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

Overexpression of Arabidopsis ESR1 Induces Initiation of Shoot Regeneration

2001 ◽  
Vol 13 (12) ◽  
pp. 2609-2618 ◽  
Author(s):  
H. Banno
Keyword(s):  
Shoot Regeneration

Stimulation of Shoot Regeneration of Vigna aconitifolia by Electrical Control

1987 ◽  
Vol 60 (4) ◽  
pp. 399-403 ◽  
Author(s):  
R. GILL ◽  
K. P. MISHRA ◽  
P. S. RAO
Keyword(s):  
Shoot Regeneration ◽  
Vigna Aconitifolia ◽  
Electrical Control ◽  
Stimulation Of

scholarly journals EFFECT OF GA3 AND PACLOBUTRAZOL ON ADVENTITIOUS SHOOT REGENERATION OF TWO PELARGONIUM SP.

2012 ◽  
pp. 187-194 ◽  
Author(s):  
L. Hamama ◽  
L. Voisine ◽  
A. Naouar ◽  
R. Gala ◽  
D. Cesbron ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Adventitious Shoot Regeneration

Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

2015 ◽  
Vol 39 (4) ◽  
pp. 975-984 ◽  
Author(s):  
Aung Htay Naing ◽  
Kyeung Il Park ◽  
Mi Young Chung ◽  
Ki Byung Lim ◽  
Chang Kil Kim
Keyword(s):  
Shoot Regeneration ◽  
Factors Affecting

scholarly journals Florigen governs shoot regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yaarit Kutsher ◽  
Michal Fisler ◽  
Adi Faigenboim ◽  
Moshe Reuveni
Keyword(s):  
Nitric Oxide ◽  
Shoot Regeneration ◽  
Ectopic Expression ◽  
Flowering Plants ◽  
Regeneration Capacity ◽  
No Donor ◽  
Tobacco Leaves ◽  
Age Related ◽  
Delayed Flowering

AbstractIt is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

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