Biological potential of Ascophyllum nodosum extract on rhizobial diversity in nodules of mothbean Vigna aconitifolia Jacq. via Amplified Ribosomal DNA Restriction Analysis

Aim: The aim of this study was to use Ascophyllum nodosum for potentially increasing the growth and rhizobial diversity in nodulating rhizobia in Vigna aconitifolia. Methodology: Different concentrations of Ascophyllum nodosum extracts (0.01%, 0.02%, 0.05%, 0.10% and 0.50%) were applied via foliar spray and on roots of Vigna aconitifolia. Growth characteristics and Amplified Ribosomal DNA Restriction Analysis were conducted to detect the morphological and molecular changes in rhizobial diversity. The restriction profiles thus obtained were used to study the rhizobial communities via Cluster analysis and Dendrogram using NTSYS-PC program and UPGMA constructed. Results: Roots treated with 0.05% Ascophyllum nodosum extract showed best growth of plants. This concentration not only proved best for the aggregation of nodules but also for obtaining enormous rhizobial diversity. Interpretation: Ascophyllum nodosum is a modern, cheap, non-toxic natural biofertilizer and Amplified Ribosomal DNA Restriction Analysis represents a favorable alternative to culture dependent method for assessing rhizobial diversity in nodulating bacteria.

Potential Use of Ascophyllum nodosum as a Biostimulant for Improving the Growth Performance of Vigna aconitifolia (Jacq.) Marechal

The fertilizers that are derived from seaweed are known as Seaweed Liquid Fertilizers (SLF). SLF is a modern, cheap, non-toxic, and natural bioactive fertilizer. Among different studied seaweeds, Ascophyllum nodosum is significant as having bioactive ingredients that potentially regulate the molecular, physiological, and biochemical processes of crop plants. In the present study, the effects of the application of different concentrations (0.00%, 0.01%, 0.02%, 0.05%, 0.10%, 0.50%, and 1.00%) of A. nodosum Extract (ANE) to the Vigna aconitifolia through roots (Pot Root Application, PRA) and on the leaves (Pot Foliar Application, PFA) were monitored via the plant growth. The lower concentrations of ANE in both the PRA and PFA experiments showed positive growth on V. aconitifolia. The 0.10% ANE stimulated the maximum shoot growth when applied through the roots, while 0.05% ANE in both PFA and PRA experiments led to an increase in the number of pods, nodules, organic content, and moisture percentage. The 0.10% ANE also increased the leaf numbers, leaf area, and photosynthetic pigments. Hence, the application of 0.05% and 0.10% of A. nodosum extract in two ways (i.e., Pot Foliar Application, PFA, and Pot Root Application, PRA) ameliorated the growth capabilities of V. aconitifolia.

Screening of Genotypes against Major Sucking Insect- Pests of Mothbean [Vigna aconitifolia] in Arid Region

Aim: Some genotypes of mothbean were screened for their comparative preference against jassids, whiteflies and thrips. Materials and Methods: The experiment was laid out during Kharif season in Randomized Block Design with three Replications. Ten genotypes (viz., RMO-225, RMO-40, RMO-423, RMO-435, RMO-257, RMO-25, RMO-141, RMO-20-36, RMO-04-01-28 and RMO-28-80) were screened against major sucking insect pests which were replicated thrice. Observations: The observations were recorded after two weeks of sowing at weekly intervals after two weeks of sowing. The pest populations were recorded on five randomly selected and tagged plants per plot in early hours when insect have minimum activity. Results: The data revealed that none of the genotypes ware found free from sucking insect pest attack. On the basis of peak population, the genotypes RMO-25 and RMO-141 were categorized as least preferred to jassids, whiteflies and thrips, whereas, RMO-435, RMO-225 and RMO-04-01-28 as highly preferred to jassids and whiteflies and RMO-435, RMO-225 and RMO-257 as highly resistant to thrips. Conclusion: It is well known that certain genotypes of crops are less attacked by a specific insect-pest than others because of natural resistance. In the integrated pest management programme, growing of varieties (RMO-25 and RMO-141) with less preference to sucking insect-pests is one of the most important tools without additional cost.

Destruction of Moth Bean (Vigna aconitifolia (jacq.) Marechal) Morphological Aspect and Biochemical Constituents during Dairy Effluent Irrigation

The current exploration is being fetched out to evaluate the consequence of dairy effluent on morphological aspect and biochemical constituents in Moth bean (Vigna aconitifolia (Jacq.) Marechal). For this, experimentation work dairy effluents were amassed from the outlet of milk processing plants. The diverse concentrations used for the effluent were Control (normal water), 5, 25, 50, 75 and 100%. The growth parameters viz., Shoot Length (SL), Roots Length (RL), Number of Leaves (NL), Total Leaf Area (TLA), Fresh Weight (FW) and Dry Weight (DW), and the biochemical issue like viz., chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, carotenoid, reducing, non-reducing sugar, total sugars, starch, amino acid, and protein content were analysed

ANALYSIS OF MEDICINAL PROPERTIES OF VIGNA ACONITIFOLIA (MATKI)

Objective: The seeds and sprouts of matki bean (Vigna aconitifolia), a common food, contain abundant nutrients with biological activities. This work was focused to analyze and compare the medicinal properties of seeds and sprouts of matki beans, with the goal of providing scientific evidence for better application of this commonly used food as a medicine. Methods: Using Soxhlet apparatus, ethanol extracts of matki bean sprouts and seeds were prepared. Anti-inflammatory activity (inhibition of protein denaturation, inhibition of heat induced hemolysis, and inhibition of protease activity), antioxidant activity, and antihypertensive activity were determined for both sprout and seed extracts. Results: The anti-inflammatory activity of sprout ethanol extract was found to be highest. Sprout ethanol extract inhibited the activity angiotensin-I-converting enzyme (ACE1) which was maximum as compared to seed extracts. Same was observed for the antioxidant activity, sprout ethanol extract showed higher activity than the seed extract. Conclusion: The results of the present study will help us to use Vigna aconitifolia as a healthy herbal alternative for the existing chemical remedies for various disorders and rule out the issue of side effects and resistance.