scholarly journals Embriogênese somática in vitro de cultivares de cenoura

Bragantia ◽  
1987 ◽  
Vol 46 (2) ◽  
pp. 303-314 ◽  
Author(s):  
Benedita Maria Rodrigues ◽  
Walter José Siqueira ◽  
João Baptista Fornasier ◽  
Rogério Salles Lisbão ◽  
Herculano Penna Medina Filho
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L

Objetivando a definição da metodologia básica para a obtenção em meio de cultura sólido de embriogênese somática, em explantes de hipocótilo e folha cotiledonar de cenoura (Daucus carota L.) cultivares Kuroda, Nantes e Brasília foi realizado um experimento em condições de laboratório, utilizando-se as concentrações de 0,5; 1,0; 2,5 e 5,0 mg/litro do ácido 2,4 diclorofenoxiacético (2,4-D) e 1,0 mg/litro do ácido beta-naftoxiacético (NOA). Pelos resultados obtidos, os explantes de hipocótilo foram em geral mais adequados para o desenvolvimento do processo de embriogênese somática nos três cultivares estudados. A melhor concentração de 2,4-D na indução da embriogênese foi de 0,5 mg/litro. O tratamento com o NOA apresentou maior freqüência de embriogênese comparativamente àqueles com o 2,4-D. Houve uma resposta diferencial dos cultivares e explantes utilizados em relação aos diferentes meios de cultura.

scholarly journals Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

2014 ◽  
Vol 38 ◽  
pp. 593-600 ◽  
Author(s):  
Krystyna GÓRECKA ◽  
Waldemar KISZCZAK ◽  
Dorota KRZYŻANOWSKA ◽  
Urszula KOWALSKA ◽  
Agata KAPUŚCIŃSKA
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L ◽  
Anther Cultures

In vitro bioaccessibility of β-carotene, Ca, Mg and Zn in landrace carrots (Daucus carota, L.)

2015 ◽  
Vol 166 ◽  
pp. 365-371 ◽  
Author(s):  
Fernanda Zaccari ◽  
María Cristina Cabrera ◽  
Ana Ramos ◽  
Ali Saadoun
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L ◽  
In Vitro Bioaccessibility ◽  
Β Carotene

scholarly journals DO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?

2020 ◽  
Vol 19 (5) ◽  
pp. 115-127
Author(s):  
Tugce OZSAN ◽  
Elif Gulsun VURAL ◽  
Ahmet Naci ONUS
Keyword(s):  
Jasmonic Acid ◽  
Daucus Carota ◽  
Activated Charcoal ◽  
Callus Formation ◽  
Formation Rate ◽  
Daucus Carota L ◽  
Positive Effects ◽  
Leaf Formation ◽  
Carrot Seed

Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.

Carrot (Daucus carota L.) In Vitro Regeneration

2010 ◽  
Vol 73 (1) ◽  
Author(s):  
Keramat Rabiei ◽  
Aleksey Polyakov ◽  
Mahmood Khodambashi ◽  
Olga Sharafova ◽  
Elena Kalashnikova ◽  
...  
Keyword(s):  
Daucus Carota ◽  
In Vitro Regeneration ◽  
Daucus Carota L

In Vitro Propagation of Carrot (Daucus Carota) L.

1970 ◽  
Vol 5 (5) ◽  
pp. 51-53 ◽  
Author(s):  
B Pant ◽  
S Manandhar
Keyword(s):  
Daucus Carota ◽  
Leaf Explants ◽  
Explant Culture ◽  
Multiple Shoot ◽  
Stem Explants ◽  
Ms Medium ◽  
Nodal Explant ◽  
Daucus Carota L ◽  
Single Shoot

In vitro method for rapid propagation of Daucus carota L. was developed. Root, stem, leaf and nodal explants were cultured on MS medium supplemented with different concentration and combinations of hormones. Multiple shoot were induced from the nodal explant of D. carota by culturing them in MS medium supplemented with 0.5 mg/l of 6-benzylaminopurine (BAP). Differentiation of shoot initiated after one week of culture, and after eight weeks of primary culture, an average of six plantlets were developed from a single shoot. The nodal explant also induced same number of multiple shoot in MS medium supplemented with 2 mg/l of BAP and 1 mg/l of α-nephthalene acetic acid (NAA). The shoots when sub-cultured in the medium supplemented with 1 mg/l NAA produced roots after five weeks of sub-culture. Root and leaf explants induced roots and callus when cultured on MS medium with NAA at the rate of 1 mg/l and 2 mg/ l. Similarly, stem explants also induced roots and callus in the same concentration of hormones whereas few multiple shoot were induced when cultured on MS supplemented with 2 mg/l of BAP and 1 mg/l of NAA. This result suggests that this methodology can be applied for the rapid and mass propagation of this species. Key words: In vitro; Propagation; MS medium; Explant; Culture. DOI: 10.3126/sw.v5i5.2656 Scientific World, Vol. 5, No. 5, July 2007 51-53

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