Peptic disease and Helicobacter pylori are highly prevalent in patients with the indeterminate form of chagas’ disease: report of 21 cases

Given that chagasic patients in the indeterminate form of this disease, can have abnormal motility of the digestive tract and immunologic abnormalities, we decided to assess the frequency of peptic disease and Helicobacter pylori (Hp) infection in these individuals. Twenty-one individuals, 13 males and 8 females, mean age 37.6 ± 11.1 years, were examined. Biopsies of the duodenum, antrum, lesser and greater gastric curvature and esophagus were performed. The endoscopic findings were of chronic gastritis in 20 (95.2%) patients, duodenal ulcer in 3 (14.3%), gastric and duodenal ulcer in 3 (14.3%), gastric ulcer alone in 1 (4.8%), esophagitis in 5 (23.8%), and duodenitis in 5 (23.8%). The diagnosis of infection by the Hp was done by the urease test and histologic examination. Hp infection was found in 20 (95.2%) individuals: in 20 out of them in the antrum, in 17 in the lesser curvature, and in 17 in the greater curvature. Hp was not found in the esophagus and duodenum. The only individual with no evidence of infection by Hp was also the only one with normal endoscopic and histologic examinations. The histologic examinations confirmed the diagnoses of gastric ulcer as peptic, chronic gastritis in 20 patients, duodenitis in 14, and esophagitis in 9. In this series the patients had a high frequency of peptic disease, which was closely associated with Hp infection

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Correlation Between Endoscopic and Histological Findings in Different Gastroduodenal Lesion and its Association with Helicobacter Pylori

Anwer Khan Modern Medical College Journal ◽

10.3329/akmmcj.v2i2.8164 ◽

1970 ◽

Vol 2 (2) ◽

pp. 6-10 ◽

Cited By ~ 1

Author(s):

A Sultana ◽

SM Badruddoza ◽

F Rahman

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Gastric Carcinoma ◽

Chronic Gastritis ◽

Normal Mucosa ◽

Endoscopic Biopsy ◽

Endoscopic Findings ◽

Duodenal Lesions ◽

H Pylori

This study had been planned to see the histomorphological pattern of gastric mucosal biopsy, demonstrate the organism H .Pylori and correlate with endoscopic findings. The study was carried out in the department of the Pathology Rajshahi Medical College, Rajshahi, Bangladesh, during the period from January 2006 to December 2007. Endoscopic biopsy specimens were obtained from 105 cases of gastro duodenal lesions. According to endoscopic findings out of 105 cases 69(65.7%) had gastric carcinoma, 06 (5.71%) had gastric ulcer, 05 (4.76%) had duodenal ulcer, 05 (476%) had gastritis and 20 (19.04%) had normal mucosa, reported as non-ulcer dyspepsia. Of the 69 endoscopically gastric carcinoma 59 (85.50%) were diagnosed histologically as adenocarcinoma and 10 (14.50%) were diagnosed histologically as chronic gastritis in which H.Pylori was positive in 27.5% and 14.50% positive in chronic gastritis. Of 06 gastric ulcer 03 (50%) was diagnosed histologically as gastritis and 03 (50%) were diagnosed histologically as gastric carcinoma. H. Pylori was positive in cent percent of gastritis. Of 05 duodenal ulcer 03 (60%) were diagnosed histologically as gastritis and 02 (40%) were diagnosed histologically as intestinal metaplasia H. Pylori was positive in 66.6% of duodenal ulcer. Of 05 gastritis were diagnosed histologically as gastritis 60% were postive for H.Pylori. Of 20 NUD 18 (90%) were diagnosed histologically as gastritis, 01 (05%) was diagnosed histologically as ulcer and 01 (05%) was diagnosed histologically as normal mucosa, all these cases no H. Pylori was found. Key Words: Helicobacter Pylori; Gastroduodenal Lesion; Association DOI: http://dx.doi.org/10.3329/akmmcj.v2i2.8164 AKMMC J 2011; 2(2): 06-10

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Prevalence of Helicobacter pylori infection, its correlation with gastroduodenal diseases and the incidence of gastric cancer in Nepal

Journal of Advances in Internal Medicine ◽

10.3126/jaim.v2i2.8777 ◽

2013 ◽

Vol 2 (2) ◽

pp. 52-60 ◽

Cited By ~ 1

Author(s):

Umid Kumar Shrestha ◽

Arnab Ghosh ◽

Vijay M Alurkar ◽

Suresh C Kohli ◽

Subash Sapkota

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Cancer Incidence ◽

Gastric Polyp ◽

Rapid Urease Test ◽

Asian Countries ◽

Urease Test ◽

H Pylori

Background and aims: The Helicobacter pylori (H. pylori) prevalence in Asian countries is highly variable, with higher seroprevalence shown in the previous studies of developing Asian countries. We aimed to determine the current H. pylori prevalence, correlate with gastroduodenal diseases and study gastric cancer incidence in Nepal. Methods: Among 3357 patients referred for endoscopy, 2820 eligible patients underwent upper gastrointestinal endoscopy with biopsy; H. pylori was considered positive when either of Rapid Urease Test (RUT) or histopathology showed positive result. Results: The H. pylori prevalence was 29.4% in overall distribution, 41.1% in gastritis and or duodenitis, 69.5% in gastric ulcer, 84.7% in duodenal ulcer, 20.8% in gastric polyp and 11.5% in gastric cancer. The H. pylori infection was significantly associated with gastritis and or duodenitis [P<0.001; Odds Ratio (OR) 1.53, 95% Confidence Interval (CI) 1.47-1.59], gastric ulcer (P<0.001; OR 18.62, 95% CI 12.40-27.81), duodenal ulcer (P<0.001; OR 48.89, 95% CI 25.23- 94.75), gastric polyp (P=0.001; OR 7.66, 95% CI 3.18-18.44) and gastric cancer (P=0.005; OR 3.78, 95% CI 1.82-7.86). The age-standardized (world) annual rate of gastric cancer in Kaski district of Nepal was 3.3 per 100,000. Conclusions: The H. pylori prevalence in Nepal was lower than that shown in the previous studies of developing Asian countries, but was significantly high in gastritis and or duodenitis, and peptic ulcers. Similarly, the gastric cancer incidence was also low in Nepal and was significantly associated with H. pylori. Further study is needed to establish the association of H. pylori with gastric cancer in Nepal. DOI: http://dx.doi.org/10.3126/jaim.v2i2.8777 Journal of Advances in Internal Medicine 2013;02(02):52-60

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Helicobacter pylori cagA gene Polymorphism in Patients with Gastroduodenal Diseases

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v43i1.35152 ◽

2018 ◽

Vol 43 (1) ◽

pp. 37-43

Author(s):

Mahbuba Chowdhury ◽

Sharmeen Ahmed ◽

A.F.M.A.L Masum Khan ◽

Shirin Tarafdar ◽

Ruhul Amin Miah

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Gastric Carcinoma ◽

Peptide Sequence ◽

Rapid Urease Test ◽

Caga Gene ◽

Urease Test ◽

H Pylori ◽

Epiya Motifs

Helicobacter pylori is a genetically diverse bacterial pathogen and its CagA gene is a major virulence factor that plays an important role in gastroduodenal pathologies. The biological function of cagA depends on tyrosine phosphorylation within the EPIYA (Glutamate-Proline-Isoleucine-Tyrosine-Alanine) motifs at the C-terminal region of the protein. This region may undergo polymorphism to give different types of EPIYA motifs. EPIYA motif diversity may provide a useful tool for prediction of H. pylori pathogenic activity and accurate determination of number and type of cagA EPIYA motifs could identify the virulent H. pylori. The aim of this study was to detect H. pylori cagA gene and its polymorphism in endoscopic gastroduodenal biopsy specimen from patients with gastroduodenal diseases in Bangladesh. This cross sectional study was carried out in the Department of Microbiology & Immunology, Bangabandhu Sheikh Mujib Medical University and Center for Advanced Research in Sciences, University of Dhaka during the period from March 2014 to February 2015. Gastric biopsies were collected from 78 patients with gastritis, duodenal ulcer, gastric ulcer and gastric carcinoma. H. pylori was identified by rapid urease test and ureC gene PCR. Presence of cagA gene and number and pattern of cagA EPIYA motif were determined by PCR. DNA sequencing was carried out to confirm the PCR detection method of cagA EPIYA motif and to analyse their peptide sequence. Among 31(39.7%) H. pylori positive cases, 19 (61.3%) were cagA gene positive in 11(55%) gastritis, 4(66.7%) duodenal ulcer, 2(66.7%) gastric ulcer and 2(100%) gastric carcinoma. A significant association was found between cagA gene and duodenal ulcer (p=˂0.05). EPIYA motif of all H. pylori cagA positive cases showed Western type cagA EPIYA ABC. No East Asian EPIYA ABD motif was found. Majority of gastroduodenal cases (57.9%) had 3 copies of EPIYA (ABC type), 26.3% had 4 copies (ABCC type) while remaining 10.5% had AC and 5.2% AB type EPIYA motif. EPIYA ABC was found in 75% of duodenal ulcer followed by 54.5% of gastritis and 50% of both gastric ulcer and gastric carcinoma patients. EPIYA ABCC motif was found in 50% of gastric ulcer and gastric carcinoma patients. Most of the EPIYA motif was EPIYA ABC and some were ABCC which has the risk of developing gastric carcinoma.

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A Clinical Significance of Helicobacter Pylori Infection in Hemophilic Children with Gastrointestinal Hemorrhage.

Blood ◽

10.1182/blood.v114.22.4442.4442 ◽

2009 ◽

Vol 114 (22) ◽

pp. 4442-4442

Author(s):

Ji Yoon Kim ◽

Yong-Mook Choi ◽

Kun Soo Lee

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Chronic Gastritis ◽

Gastrointestinal Hemorrhage ◽

Conflicts Of Interest ◽

Helicobacter Pylori Infection ◽

Rapid Urease Test ◽

Urease Test ◽

Life Threatening ◽

H Pylori

Abstract Abstract 4442 Purpose For hemophiliacs gastrointestinal hemorrhage is a life-threatening complication and can be caused by the Helicobacter pylori infection. In this study, the prevalence of H. pylori infection among child hemophiliacs with gastrointestinal hemorrhage and the recurrence rate after eradication H. pylori treatment was investigated. Patients and Methods Seven children with hemophilia A with hematemesis (age, 5.3∼17.0 year) were evaluated for the causes of gastrointestinal hemorrhage and the detection of H. pylori. Gastroendoscopy was done to find the bleeding focus and for further evaluation including rapid urease test and mucosal biopsy. Result: Four patients had dyspepsia and abdominal pain for several weeks or months prior to hematemesis. Three patients did not show any signs of bleeding. From gastroendoscopy, four patients were diagnosed as duodenal ulcer, one as H. pylori associated chronic gastritis and one as hemorrhagic gastritis. One patient showing a normal finding was diagnosed with adenoid hemorrhage after nasopharyngoscopy. H. pylori infection was found in four of six patients with GI bleeding (3, duodenal ulcer; 1, H. pylori associated chronic gastritis). The patients with H. pylori infection had an eradication treatment of triple therapy and no recurrence happened. Conclusion In child hemophiliacs, H. pylori should also be considered as an important cause of gastrointestinal hemorrhage. The recurrence of the infection and gastrointestinal hemorrhage can be prevented with eradication of H. pylori. Screening test for H. pylori would be needed in child hemophiliacs in endemic area. Disclosures: No relevant conflicts of interest to declare.

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Detection of Helicobacter Pylori in Endoscopic Biopsies in Sudan

Tropical Doctor ◽

10.1177/004947559402400407 ◽

1994 ◽

Vol 24 (4) ◽

pp. 161-163 ◽

Cited By ~ 13

Author(s):

Yousra A Azim Mirghani ◽

Salah Ahmed ◽

Mohamed Ahmed ◽

Mustafa O Ismail ◽

Sulieman Salih Fedail ◽

...

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Gastric Ulcer ◽

Normal Control ◽

Oesophageal Varices ◽

Rapid Urease Test ◽

Control Subjects ◽

Biopsy Specimens ◽

Urease Test ◽

H Pylori

We studied the prevalence of Helicobacter pylori in Sudanese subjects with gastroduodenal inflammation. H. pylori was looked for in biopsy specimens taken from the antrum by two methods: rapid urease test [Campylobacter-like organism (CLO) test] and culture using Skirrow's selective supplement. One hundred subjects were studied. H. pylori was found in 80% of patients with gastritis, 56% of patients with duodenal ulcer, 60% of patients with duodenitis and 16% of normal control subjects. It was neither detected in patients with gastric ulcer, nor in patients with oesophagitis or in those with oesophageal varices due to schistosomiasis, when using culture. However, it was found in 50% of patients with oesophagitis, when using CLO test.

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Association of IL1B -511C/-31T haplotype and Helicobacter pylori vacA genotypes with gastric ulcer and chronic gastritis

BMC Gastroenterology ◽

10.1186/1471-230x-10-126 ◽

2010 ◽

Vol 10 (1) ◽

Cited By ~ 38

Author(s):

Dinorah N Martínez-Carrillo ◽

Elvira Garza-González ◽

Reyes Betancourt-Linares ◽

Trinidad Mónico-Manzano ◽

Cuauhtémoc Antúnez-Rivera ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Ulcer ◽

Chronic Gastritis

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Helicobacter pyloriAnti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Canadian Journal of Gastroenterology ◽

10.1155/2002/589087 ◽

2002 ◽

Vol 16 (8) ◽

pp. 527-532 ◽

Cited By ~ 13

Author(s):

M Fatih Abasiyanik ◽

Ersan Sander ◽

Barik A Salih

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Chronic Gastritis ◽

Molecular Weights ◽

Cancer Antigens ◽

Gastroduodenal Disease ◽

H Pylori ◽

Asymptomatic Subjects

BACKGROUND: Several reports have shown the prevalence of anti-CagA antibodies to be associated with the development of peptic ulcer diseases, while others have indicated that there is no such association.AIM: To examine the prevalence of antibodies to CagA and otherHelicobacter pyloriantigens in symptomatic and asymptomatic subjects in Turkey.SUBJECTS AND METHODS: Sixty-six symptomatic subjects, 16 to 74 years of age, were examined forH pyloriby biopsy-based tests and ELISA. One hundred nineteen asymptomatic subjects, 20 to 65 years of age, were also tested serologically for the presence ofH pylori. Samples from both groups that were found to be positive forH pyloriby ELISA were then tested by immunoblotting.RESULTS: Fifty-four (82%) symptomatic subjects and 76 (64%) asymptomatic subjects were found to beH pylori-positive by ELISA. Samples from 30 symptomatic subjects who were found to beH pylori-positive by ELISA were analyzed by immunoblotting. Antibodies to CagA (116 kDa) antigen were detected in immunoblots of 11 of 14 (79%) with chronic gastritis, 12 of 13 (92%) with duodenal ulcer and three of three (100%) with gastric cancer. Antigens of the following molecular weights were also detected in these 30 subjects: 89 kDa (VacA) in 21 (70%), 37 kDa in 21 (70%), 35 kDa in 19 (63%), 30 kDa in 27 (90%) and 19.5 kDa in 19 (63%). Immunoblots of 40 ELISA-positive asymptomatic subjects showed that 33 (83%) had antibodies to CagA antigen, 26 (65%) to VacA antigen, 30 (75%) to a 37 kDa antigen, 30 (75%) to a 35 kDa antigen, 39 (98%) to a 30 kDa antigen and 36 (90%) to a 19.5 kDa antigen.CONCLUSIONS: Antibodies to CagA antigen were prevalent in both groups, regardless of the presence of gastroduodenal disease.

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Anti-Helicobacter pylori and Anti-Inflammatory Effects and Constituent Analysis of Modified Xiaochaihutang for the Treatment of Chronic Gastritis and Gastric Ulcer

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/6810369 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15 ◽

Cited By ~ 2

Author(s):

Xin Chen ◽

Lijuan Hu ◽

Huanhuan Wu ◽

Wei Liu ◽

Shuhe Chen ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Ulcer ◽

Chronic Gastritis ◽

Gastric Ulcers ◽

Etoac Extract ◽

Rat Paw Edema ◽

Edema Model ◽

Mouse Ear ◽

Anti Inflammatory ◽

Rat Paw

Chronic gastritis and gastric ulcers are prevalent throughout the world and are considered to be a global health problem. Modified Xiaochaihutang (MXCHT) prescription is broadly used in traditional medicine hospital for the treatment of gastritis. In order to assess the anti-Helicobacter pylori (H. pylori) effect of MXCHT, agar diffusion method in vitro and fluid dilution method for the minimal inhibitory concentration (MIC) were established. The anti-inflammatory effects were then evaluated using mouse ear edema model and rat paw edema model. The ethanol-induced gastric ulcer method was employed to verify the gastroprotective effect of active extracts in MXCHT. HPLC-TOF-MS/MS was used for analyzing the possible active constituents after oral administration of effective extracts in ethanol-induced gastric ulcer models. MXCHT and 4 different extracts of the bacterial inhibition diameter and MIC were dramatically decreased compared with control group, showing anti-Helicobacter pylori effects. High dose groups of MXCHT, water extract, EtOAc extract, and n-BuOH extract displayed significant anti-inflammatory effects in xylene-induced mouse ear edema model and carrageenan-induced rat paw edema model test. MXCHT and all active extracts exhibited gastroprotective activity and prevented gastric lesions induced by ethanol in rats. 4 prototype components and 4 metabolites were identified after oral administration of EtOAc extract. In addition, 6 prototype components and 6 metabolites were identified in n-BuOH extract. MXCHT, EtOAc extract, and n-BuOH extract demonstrate gastroprotective effects through anti-Helicobacter pylori and anti-inflammatory activities. Thus, this prescription may be a suitable natural source for the prevention and treatment of chronic gastritis and gastric ulcers.

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Statistical analysis of the сhronic gastritis in students

Wiadomości Lekarskie ◽

10.36740/wlek202002129 ◽

2020 ◽

Vol 73 (2) ◽

pp. 360-364

Author(s):

Aleksandr V. Kharchenko ◽

Nataliya V. Kharchenko ◽

Petro M. Makarenko ◽

Lyudmyla M. Sakharova ◽

Pavlo V. Khomenko ◽

...

Keyword(s):

Helicobacter Pylori ◽

Statistical Analysis ◽

Gastric Mucosa ◽

Atrophic Gastritis ◽

Mucous Membrane ◽

Chronic Gastritis ◽

Bacterial Contamination ◽

The Body ◽

Leukocyte Infiltration ◽

Lesser Curvature

The aim: The aim of the study is a statistical analysis of the mucosa of the stomach affected by Helicobacter pylori in young people studying at the university. Materials and methods: The work contains the results of the study of chronic gastritis of type B in university volunteer students. The study was attended by students of 1-4 courses, aged 17 to 25 years, a total of 50 people. Among them were 28 men and 22 women. Results: Various forms of chronic gastritis were found in the mucosa of the topographic-anatomical sections of the stomach, 90% of which were associated with Helicobacter pylori (HP). In all departments there is a different amount of common forms of chronic gastritis. In the pyloric section only atrophic gastritis was detected – 31.0 ± 8.5. Atrophic gastritis was also dominant on the lesser curvature – 32.3 ± 7.8, but its forms were significantly (p <0.5) less pronounced than in the pyloric section. In the area of the body, the above variants of chronic gastritis were found in 34.3 ± 8.7 cases, and the majority were flat erosive gastritis 51.0 ± 9.3. There is a tendency to reduce the degree of bacterial contamination of the gastric mucosa from its pyloric section and the lesser curvature to the walls of the body. With a decrease in the degree of bacterial contamination of the gastric mucosa, the degree of leukocyte infiltration also decreases. Between the degree of contamination of the mucous membrane of Helicobacter pylori and the degree of leukocyte infiltration of the mucous membrane, the Pearson correlation coefficient is rxy – 0,935, the correlation is very strong, the coefficient of determination is D=rxy^2 – 0,874, the statistically significant dependence on the probability is 0.99. Conclusions: Atrophic or hyperplastic gastritis associated with HP is found in the gastric mucosa, respectively, 90% of cases. The degree of bacterial contamination correlates with the degree of leukocyte infiltration of the gastric mucosa. Atrophic or hyperplastic gastritis Helicobacter pylori-associated is a common disease of people in young and working age.

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