A preliminary analysis of the population genetics and molecular phylogenetics of Onchocerca volvulus (Nematoda: Filarioidea) using nuclear ribosomal second internal transcribed spacer sequences

Summary The eggs recovered during faecal screening of Asiatic lions (kept at MC Zoological Park, Punjab, India) were delineated as Toxascaris leonina eggs based on morphometric and molecular studies (polymerase chain reaction targeting internal transcribed spacer sequences). Therapeutic management with fenbendazole @10 mg/kg body weight, once daily orally for three consecutive days proved ineffective with maximum faecal egg count reduction (FECR) on day 3 post treatments (69.35 %). But, therapeutic intervention with extended period dose schedule (5 consecutive days) with fenbendazole (@10 mg/kg body weight) proved effective and showed a maximum FECR of 95.34 % at day 7 post treatments. But, when ivermectin (@100μg/kg body weight) was given orally on three alternate days, proved effective as FECR of 95.74 % was recorded at day 7 post treatments. Thus, present study highlights the molecular confi rmation of T. leonina and its management using fenbendazole and ivermectin in Asiatic lions.

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Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762011000100001 ◽

2011 ◽

Vol 106 (1) ◽

pp. 01-08 ◽

Cited By ~ 16

Author(s):

Fabiana Tavares Vesgueiro ◽

Bruna Demari-Silva ◽

Rosely dos Santos Malafronte ◽

Maria Anice Mureb Sallum ◽

Mauro Toledo Marrelli

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Intragenomic Variation ◽

Second Internal Transcribed Spacer

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Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.

Genetic Resources and Crop Evolution ◽

10.1007/s10722-014-0086-5 ◽

2014 ◽

Vol 61 (5) ◽

pp. 909-925 ◽

Cited By ~ 8

Author(s):

Behrouz Shiran ◽

Soghra Kiani ◽

Deepmala Sehgal ◽

Akram Hafizi ◽

Tanvir ul-Hassan ◽

...

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Nuclear Ribosomal Dna ◽

Taxonomic History ◽

Internal Transcribed Spacer Sequences

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Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

Helminthologia ◽

10.2478/s11687-007-0002-z ◽

2007 ◽

Vol 44 (2) ◽

pp. 43-46 ◽

Cited By ~ 5

Author(s):

D. Kuznetsov ◽

N. Kuznetsova

Keyword(s):

Dna Sequence ◽

Intraspecific Variation ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Moscow Region ◽

Dna Sequence Data ◽

Interspecific Differences ◽

Second Internal Transcribed Spacer ◽

First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

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