scholarly journals Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods

2009 ◽  
Vol 104 (4) ◽  
pp. 576-579 ◽  
Author(s):  
AD Schlindwein ◽  
CMO Simões ◽  
CRM Barardi
2006 ◽  
Vol 97 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Khaoula Belguith ◽  
Abdennaceur Hassen ◽  
Mahjoub Aouni

1999 ◽  
Vol 39 (7) ◽  
pp. 243-250 ◽  
Author(s):  
Joana Azeredo ◽  
Valentina Lazarova ◽  
Rosário Oliveira

To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization. Glutaraldehyde is a bifunctional reagent that binds to cell walls avoiding their permeabilization and the biofilm matrix is solubilized in the solution.


2018 ◽  
Vol 5 (2) ◽  
pp. 38 ◽  
Author(s):  
David De la Torre ◽  
Luis Nuñez ◽  
Claudete Astolfi-Ferreira ◽  
Antonio Piantino Ferreira

2019 ◽  
Vol 120 ◽  
pp. 166-175 ◽  
Author(s):  
Małgorzata Wilk ◽  
Aneta Magdziarz ◽  
Kandasamy Jayaraman ◽  
Monika Szymańska-Chargot ◽  
Iskender Gökalp

2021 ◽  
pp. 107238
Author(s):  
Ana Luísa Alves ◽  
Francisco Javier Fraguas ◽  
Ana Cristina Carvalho ◽  
Jesús Valcárcel ◽  
Ricardo Isaac Pérez-Martín ◽  
...  

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