Activated sludge morphology characterization through an image analysis procedure

Quantification of filamentous bacteria in activated sludge systems can be made by manual counting under a microscope or by the application of various automated image analysis procedures. The latter has been significantly developed in the last two decades. In this work a new method based upon automated image analysis techniques was elaborated and presented. It consisted of three stages: (a) Neisser staining, (b) grabbing of microscopic images, and (c) digital image processing and analysis. This automated image analysis procedure possessed the features of novelty. It simultaneously delivered data about aggregates and filaments in an individual calculation routine, which is seldom met in the procedures described in the literature so far. What is more important, the macroprogram performing image processing and calculation of morphological parameters was written in the same software which was used for grabbing of images. Previously published procedures required using two different types of software, one for image grabbing and another one for image processing and analysis. Application of this new procedure for the quantification of filamentous bacteria in the full-scale as well as laboratory activated sludge systems proved that it was simple, fast and delivered reliable results.

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Development of an image analysis procedure for identifying protozoa and metazoa typical of activated sludge system

Water Research ◽

10.1016/j.watres.2007.02.006 ◽

2007 ◽

Vol 41 (12) ◽

pp. 2581-2589 ◽

Cited By ~ 26

Author(s):

Y.P. Ginoris ◽

A.L. Amaral ◽

A. Nicolau ◽

M.A.Z. Coelho ◽

E.C. Ferreira

Keyword(s):

Image Analysis ◽

Activated Sludge ◽

Analysis Procedure ◽

Activated Sludge System

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Optimization of an Image Analysis Procedure for Monitoring Activated Sludge Settleability

IFAC Proceedings Volumes ◽

10.1016/s1474-6670(17)32605-8 ◽

2004 ◽

Vol 37 (3) ◽

pp. 345-350 ◽

Cited By ~ 1

Author(s):

R. Jenné ◽

E.N. Banadda ◽

I.Y. Smets ◽

G. Gins ◽

M. Mys ◽

...

Keyword(s):

Image Analysis ◽

Activated Sludge ◽

Analysis Procedure ◽

Sludge Settleability

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Activated sludge image analysis system: monitoring settleability and effluent clarity

Water Science & Technology ◽

10.2166/wst.2005.0694 ◽

2005 ◽

Vol 52 (10-11) ◽

pp. 193-199 ◽

Cited By ~ 4

Author(s):

R. Jenné ◽

E.N. Banadda ◽

I.Y. Smets ◽

A. Bamelis ◽

L. Verdickt ◽

...

Keyword(s):

Image Analysis ◽

Activated Sludge ◽

Suspended Solids ◽

Analysis Procedure ◽

Volume Index ◽

Image Analysis System ◽

Sludge Volume Index ◽

Analysis System ◽

The One

A fully automized image analysis procedure is presented for fast and reliable characterization of the activated sludge composition. In previous research, relations between filament abundance and floc shape on the one hand, and the Sludge Volume Index on the other hand were sought. This work aims at demonstrating the use of the image analysis information as an indication for the amount of suspended solids in the effluent.

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End-face image analysis procedure for the calibration of optical fibre geometry test sets

10.3403/30347115 ◽

2017 ◽

Keyword(s):

Image Analysis ◽

Optical Fibre ◽

Face Image ◽

Analysis Procedure ◽

Face Image Analysis ◽

Test Sets ◽

Geometry Test

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Application of Image Analysis System for Study of Activated Sludge Flocs

Water Quality Research Journal ◽

10.2166/wqrj.1986.010 ◽

1986 ◽

Vol 21 (1) ◽

pp. 130-140 ◽

Cited By ~ 4

Author(s):

Da-hong Li ◽

J. J. Ganczarczyk

Keyword(s):

Image Analysis ◽

Activated Sludge ◽

Statistical Processing ◽

Equivalent Diameter ◽

Image Analysis System ◽

Sectional Area ◽

Cross Sectional ◽

Computerized Image Analysis ◽

Sludge Flocs ◽

Analysis System

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.

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Solids Concentrations in a Treatment Plant Effluent

Water Science & Technology ◽

10.2166/wst.1992.0783 ◽

1992 ◽

Vol 26 (9-11) ◽

pp. 2543-2546

Author(s):

M. Defrain ◽

F. Schmidt

Keyword(s):

Waste Water ◽

Activated Sludge ◽

Federal Republic ◽

Treatment Plant ◽

Analysis Procedure ◽

Process Water ◽

Federal Republic Of Germany ◽

Output Variable ◽

Sludge Treatment ◽

Sewerage Network

In the calculations customarily used to dimension nitrification and denitrification plants in the Federal Republic of Germany, solids in the influent of the biological stage represent a significant output variable. Numerous modified methods based on nationally and internationally standardized analysis procedures are available to determine solids concentrations in waste-water. Tests showed that different values are measured depending on the method of analysis employed. Harmonization of the analysis procedure is advisable to ensure comparable conditions for the calculation of activated sludge tank volumes. The solids reaching a treatment plant originate from two main sources. Filterable materials are passed to the treatment plant via the preceding sewerage network with its industrial and commercial users, but are also carried by process water from sludge treatment. The influence of process water on wastewater composition is illustrated by reference to the Wuppertal-Buchenhofen treatment plant.

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