scholarly journals Development of an image analysis procedure for identifying protozoa and metazoa typical of activated sludge system

2007 ◽  
Vol 41 (12) ◽  
pp. 2581-2589 ◽  
Author(s):  
Y.P. Ginoris ◽  
A.L. Amaral ◽  
A. Nicolau ◽  
M.A.Z. Coelho ◽  
E.C. Ferreira
2014 ◽  
Vol 70 (6) ◽  
pp. 955-963 ◽  
Author(s):  
Ewa Liwarska-Bizukojc ◽  
Marcin Bizukojc ◽  
Olga Andrzejczak

Quantification of filamentous bacteria in activated sludge systems can be made by manual counting under a microscope or by the application of various automated image analysis procedures. The latter has been significantly developed in the last two decades. In this work a new method based upon automated image analysis techniques was elaborated and presented. It consisted of three stages: (a) Neisser staining, (b) grabbing of microscopic images, and (c) digital image processing and analysis. This automated image analysis procedure possessed the features of novelty. It simultaneously delivered data about aggregates and filaments in an individual calculation routine, which is seldom met in the procedures described in the literature so far. What is more important, the macroprogram performing image processing and calculation of morphological parameters was written in the same software which was used for grabbing of images. Previously published procedures required using two different types of software, one for image grabbing and another one for image processing and analysis. Application of this new procedure for the quantification of filamentous bacteria in the full-scale as well as laboratory activated sludge systems proved that it was simple, fast and delivered reliable results.


2004 ◽  
Vol 37 (3) ◽  
pp. 345-350 ◽  
Author(s):  
R. Jenné ◽  
E.N. Banadda ◽  
I.Y. Smets ◽  
G. Gins ◽  
M. Mys ◽  
...  

2005 ◽  
Vol 52 (10-11) ◽  
pp. 193-199 ◽  
Author(s):  
R. Jenné ◽  
E.N. Banadda ◽  
I.Y. Smets ◽  
A. Bamelis ◽  
L. Verdickt ◽  
...  

A fully automized image analysis procedure is presented for fast and reliable characterization of the activated sludge composition. In previous research, relations between filament abundance and floc shape on the one hand, and the Sludge Volume Index on the other hand were sought. This work aims at demonstrating the use of the image analysis information as an indication for the amount of suspended solids in the effluent.


2018 ◽  
Author(s):  
Gede H Cahyana

As a part of activated sludge system, oxidation ditch has a special form like a canal without end point. Rotor is used to diffuse oxygen from air to the water.


1974 ◽  
Vol 9 (1) ◽  
pp. 235-249 ◽  
Author(s):  
S.G. Nutt ◽  
K.L. Murphy

Abstract Conventional wastewater parameters are accepted as inadequate estimates of the condition of activated sludge but numerous other indices have been suggested as specific measurements of the activity and viability of the biomass. Literature in the related fields of microbiology and biochemistry were reviewed in order to select the most appropriate activity parameters for application to a heterogeneous biological material. Modified analytical methods were applied to a well-controlled biological system containing a single predominant bacterial species to evaluate the relative merit of each as an indicator of viability and activity. The potential of each parameter in a complex heterogeneous system was determined by monitoring each index in a bench activated sludge system. The predominant culture studies indicated that the ATP content of the biomass and the dehydrogenase activity were potential indicators of cell viability in a simple system. However, in the complex activated sludge system, only the ATP content showed significant correlation to the organic carbon removal rate.


1986 ◽  
Vol 21 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Da-hong Li ◽  
J. J. Ganczarczyk

Abstract The computerized image analysis system has been successfully used for determination and statistical processing of the following geometric characteristics of activated sludge flocs: longest dimension, breadth, equivalent diameter, cross-sectional area, perimeter, elongation, and circularity. These parameters could be effectively and precisely determined by the system applied. In addition, the studied method, as compared to direct microscope observation and photography floc-sizing methods, was found to be more accurate, less time-consuming, and less dependent on the investigators.


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