scholarly journals Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence

2006 ◽  
Vol 29 (1) ◽  
pp. 79-82
Author(s):  
Lígia S.L.S. Mota ◽  
Rogério A. Curi ◽  
Darío A. Palmieri ◽  
Alexandre S. Borges ◽  
Catalina R. Lopes ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bubalus Bubalis ◽  
Myostatin Gene ◽  
Sequence Characterization ◽  
Coding Regions ◽  
Murrah Buffaloes

scholarly journals Molecular cloning and sequence characterization of two genes, DQA1 and DQA2,from the Chinese yakow (Bos grunniens × Bos taurus)

2017 ◽  
Vol 41 ◽  
pp. 136-141
Author(s):  
Dongmei XI ◽  
Sameeullah MEMON ◽  
Guozhi LI ◽  
Xiangying LIU ◽  
Chao SU ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Bos Taurus ◽  
Sequence Characterization ◽  
Bos Grunniens

Molecular Characterization of the TLR2 Gene in Datong Yak

2019 ◽  
Author(s):  
Shuai Peng ◽  
Lang Chen ◽  
Tian Yu Zheng ◽  
Li Zhang ◽  
Zhuo Li ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Amino Acid Sequences ◽  
Regulation Mechanism ◽  
Coding Region ◽  
Sequence Characterization ◽  
Tir Domain ◽  
Tlr2 Gene ◽  
Nucleotide Mutation ◽  
Leucine Rich Repeats

The coding region of Datong yak’s TLR2 gene was amplified and subjected to sequence characterization. The coding region of the Datong yak TLR2 gene comprised a single ORF of 2355 nucleotides that coded for 784 amino acids with translatable products. The coding region of the TLR2 gene of the Datong yak contained two nucleotide mutation sites, namely, G677A and G1587A. G677A exhibited a missense mutation. After comparing nucleotide and amino acid sequences among related species and constructing the phylogenetic relationships, Datong yak sequences were shown to be highly similar to those of Bos taurus. The Datong yak TLR2 protein simultaneously possessed leucine-rich repeats, a TIR domain and an aldehyde dehydrogenase active site. Results showed that the protein plays an important role in the body’s immune regulation mechanism.

Isolation and Sequence Characterization of Mammary Derived Growth Inhibitor Gene of Riverine Buffalo (Bubalus Bubalis)

2007 ◽  
Vol 18 (2) ◽  
pp. 123-130 ◽  
Author(s):  
M. Mukesh ◽  
B. P. Mishra ◽  
R. S. Kataria ◽  
S. P. S. Ahlawat ◽  
R. C. Sobti
Keyword(s):  
Growth Inhibitor ◽  
Bubalus Bubalis ◽  
Sequence Characterization ◽  
Inhibitor Gene

scholarly journals First molecular characterization of Cryptosporidium and Giardia from bovines (Bos taurus and Bubalus bubalis) in Sri Lanka: unexpected absence of C. parvum from pre-weaned calves

2014 ◽  
Vol 7 (1) ◽  
pp. 75 ◽  
Author(s):  
Harshanie Abeywardena ◽  
Aaron R Jex ◽  
Anson V Koehler ◽  
RPV Rajapakse ◽  
Kanchana Udayawarna ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Molecular Characterization ◽  
Bos Taurus ◽  
Bubalus Bubalis

scholarly journals Possible Mutations Expressed in Double Musculature Phenotype

2021 ◽  
pp. 1-5
Author(s):  
María Angélica Reyes Páez ◽  
◽  
Natalia Johana Rojas Tovar ◽  
Geovanny MendozaSánchez ◽  
Marcela Fernández Muñoz ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Muscle Development ◽  
Bos Taurus ◽  
Interspecific Variation ◽  
Bubalus Bubalis ◽  
Holstein Cattle ◽  
Myostatin Gene ◽  
Double Muscle ◽  
The 19Th Century ◽  
Normal Controls

Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.

Identification and sequence characterization of melanocortin 1 receptor gene (MC1R) in Bos indicus versus (Bos taurus X Bos indicus)

2019 ◽  
Vol 31 (4) ◽  
pp. 283-294 ◽  
Author(s):  
Talla Sridhar Goud ◽  
Ramesh Chandra Upadhyay ◽  
Suneel Kumar Onteru ◽  
Vijaya Bhaskar Reddy Pichili ◽  
Kiranmai Chadipiralla
Keyword(s):  
Bos Taurus ◽  
Receptor Gene ◽  
Bos Indicus ◽  
Melanocortin 1 Receptor ◽  
Sequence Characterization

Sequence Characterization of Forebrain Embryonic Zinc Finger-like (FEZL) Gene in Indian Zebu (Bos indicus) Cattle and their Crossbreds

2021 ◽  
Author(s):  
S. Rajesh Kumar ◽  
I.D. Gupta ◽  
S. Goyal ◽  
Kathiravan Periasamy ◽  
A. Verma ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Neuronal Development ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Complete Sequence ◽  
Gene Markers ◽  
Sequence Characterization ◽  
Exon 1 ◽  
Karan Fries

Background: Forebrain embryonic zinc finger-like (FEZL) gene is an important candidate associated with mastitis resistance in dairy cattle. FEZL is involved in transcriptional regulation of neuronal development and there exists a crosstalk between neuronal development and immunity via downstream cytokine expression. A single glycine insertion into glycine stretch of FEZL gene has large effect on downstream cytokine pathway making the cows susceptible to mastitis. The present study was aimed to sequence characterize FEZL gene in Sahiwal (Bos indicus) and Karan Fries (Bos inidcus X Bos taurus) cattle.Methods: Sequence characterization of bovine FEZL gene was carried out by primer walking method. Ten sets of oligonucleotide primers were designed to synthesize overlapping fragments and generate the complete sequence of about 3.7 kb covering all exons and 5’ upstream regulatory and flanking regions.Result: A total of eight nucleotide variations including three INDELS and five substitution mutations were observed among FEZL gene sequences of Bos taurus, Bos indicus (Sahiwal) and Bos taurus X Bos indicus (Karan Fries) cattle. The conceptualized amino acid sequence of bovine FEZL gene in Sahiwal and Karan Fries cattle was found to have 13 tandem Glycine residues and a serine to proline change within exon 1 region. The percent identity of FEZL gene of Sahiwal and Karan Fries cattle was 99% with that of Bos taurus, 95% with dog, horse and pig, 94% with human, 93% with rabbit, 92% with marmoset, 89% with rat and 79% with chicken. Sequence characterization of ~0.7 kb 5’ flanking region showed that it is highly conserved among bovines and resulted in prediction of six putative sites for binding of transcription factors (including Elk-1, Oct-1, HNF4, Lmo2 complex, GATA-3 and Nkx2-5). Elucidation of Bos indicus FEZL gene will further form the basis to identify candidate gene markers for association with mastitis resistance/susceptibility in cattle.

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