Sequence Characterization of Forebrain Embryonic Zinc Finger-like (FEZL) Gene in Indian Zebu (Bos indicus) Cattle and their Crossbreds

2021 ◽  
Author(s):  
S. Rajesh Kumar ◽  
I.D. Gupta ◽  
S. Goyal ◽  
Kathiravan Periasamy ◽  
A. Verma ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Neuronal Development ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Complete Sequence ◽  
Gene Markers ◽  
Sequence Characterization ◽  
Exon 1 ◽  
Karan Fries

Background: Forebrain embryonic zinc finger-like (FEZL) gene is an important candidate associated with mastitis resistance in dairy cattle. FEZL is involved in transcriptional regulation of neuronal development and there exists a crosstalk between neuronal development and immunity via downstream cytokine expression. A single glycine insertion into glycine stretch of FEZL gene has large effect on downstream cytokine pathway making the cows susceptible to mastitis. The present study was aimed to sequence characterize FEZL gene in Sahiwal (Bos indicus) and Karan Fries (Bos inidcus X Bos taurus) cattle.Methods: Sequence characterization of bovine FEZL gene was carried out by primer walking method. Ten sets of oligonucleotide primers were designed to synthesize overlapping fragments and generate the complete sequence of about 3.7 kb covering all exons and 5’ upstream regulatory and flanking regions.Result: A total of eight nucleotide variations including three INDELS and five substitution mutations were observed among FEZL gene sequences of Bos taurus, Bos indicus (Sahiwal) and Bos taurus X Bos indicus (Karan Fries) cattle. The conceptualized amino acid sequence of bovine FEZL gene in Sahiwal and Karan Fries cattle was found to have 13 tandem Glycine residues and a serine to proline change within exon 1 region. The percent identity of FEZL gene of Sahiwal and Karan Fries cattle was 99% with that of Bos taurus, 95% with dog, horse and pig, 94% with human, 93% with rabbit, 92% with marmoset, 89% with rat and 79% with chicken. Sequence characterization of ~0.7 kb 5’ flanking region showed that it is highly conserved among bovines and resulted in prediction of six putative sites for binding of transcription factors (including Elk-1, Oct-1, HNF4, Lmo2 complex, GATA-3 and Nkx2-5). Elucidation of Bos indicus FEZL gene will further form the basis to identify candidate gene markers for association with mastitis resistance/susceptibility in cattle.

Identification and sequence characterization of melanocortin 1 receptor gene (MC1R) in Bos indicus versus (Bos taurus X Bos indicus)

2019 ◽  
Vol 31 (4) ◽  
pp. 283-294 ◽  
Author(s):  
Talla Sridhar Goud ◽  
Ramesh Chandra Upadhyay ◽  
Suneel Kumar Onteru ◽  
Vijaya Bhaskar Reddy Pichili ◽  
Kiranmai Chadipiralla
Keyword(s):  
Bos Taurus ◽  
Receptor Gene ◽  
Bos Indicus ◽  
Melanocortin 1 Receptor ◽  
Sequence Characterization

scholarly journals Molecular cloning and sequence characterization of two genes, DQA1 and DQA2,from the Chinese yakow (Bos grunniens × Bos taurus)

2017 ◽  
Vol 41 ◽  
pp. 136-141
Author(s):  
Dongmei XI ◽  
Sameeullah MEMON ◽  
Guozhi LI ◽  
Xiangying LIU ◽  
Chao SU ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Bos Taurus ◽  
Sequence Characterization ◽  
Bos Grunniens

Characterization of Exon 3 of PROP1 gene and Screening of H173R polymorphism in Karan Fries bulls

2016 ◽  
Author(s):  
M. R. Vineeth ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Ankit Magotra ◽  
Rakesh Kumar ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Pcr Amplification ◽  
Multiple Alignment ◽  
Reference Sequence ◽  
Target Region ◽  
Region Sequence ◽  
Blast Analysis ◽  
Prop1 Gene ◽  
Karan Fries

Present study was done in thirty Karan Fries bulls to characterize the Exon 3 of PROP1 gene and to screen for the H173R polymorphism as well as other variations including reported and novel SNPs in the targeted region. The exon 3 was characterized by sequencing the amplicons obtained after PCR amplification using custom designed primers. The BLAST analysis of the obtained sequence yielded 100% and 99% homology with sequences of Bos taurus and bison respectively. The multiple alignment of the target region sequence with Bos taurus reference sequence revealed that the bulls under the study were free of H173R mutations. No variations were observed thus giving the targeted region a highly conserved one.

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

Molecular characterization and SNP identification in HSPB6 gene in Karan Fries (Bos taurus x Bos indicus) cattle

2017 ◽  
Vol 49 (5) ◽  
pp. 1059-1063
Author(s):  
Rakesh Kumar ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Ragini Kumari ◽  
Nishant Verma
Keyword(s):  
Molecular Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Karan Fries

Molecular Characterization of the TLR2 Gene in Datong Yak

2019 ◽  
Author(s):  
Shuai Peng ◽  
Lang Chen ◽  
Tian Yu Zheng ◽  
Li Zhang ◽  
Zhuo Li ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Amino Acid Sequences ◽  
Regulation Mechanism ◽  
Coding Region ◽  
Sequence Characterization ◽  
Tir Domain ◽  
Tlr2 Gene ◽  
Nucleotide Mutation ◽  
Leucine Rich Repeats

The coding region of Datong yak’s TLR2 gene was amplified and subjected to sequence characterization. The coding region of the Datong yak TLR2 gene comprised a single ORF of 2355 nucleotides that coded for 784 amino acids with translatable products. The coding region of the TLR2 gene of the Datong yak contained two nucleotide mutation sites, namely, G677A and G1587A. G677A exhibited a missense mutation. After comparing nucleotide and amino acid sequences among related species and constructing the phylogenetic relationships, Datong yak sequences were shown to be highly similar to those of Bos taurus. The Datong yak TLR2 protein simultaneously possessed leucine-rich repeats, a TIR domain and an aldehyde dehydrogenase active site. Results showed that the protein plays an important role in the body’s immune regulation mechanism.

Phenotypic characterization of the Saudi Arabian Hassawi cattle breed

1997 ◽  
Vol 21 ◽  
pp. 35-42 ◽  
Author(s):  
T. A. Mohammed
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Cattle Breed ◽  
Farming System ◽  
Phenotypic Characterization ◽  
Zebu Cattle ◽  
Body Conformation ◽  
High Feed ◽  
Farming Families

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.

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