scholarly journals Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)

2005 ◽  
Vol 36 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Carla Roberta Freschi ◽  
Luiz Fernando de Oliveira e Silva Carvalho ◽  
Celso José Bruno de Oliveira
Keyword(s):  
Polymerase Chain Reaction ◽  
Salmonella Typhimurium ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Swine Feces ◽  
Polymerase Chain ◽  
Dna Extraction Methods

scholarly journals Evaluation of DNA Extraction Methods for Detection of <i>Leishmania</i> by Polymerase Chain Reaction

2020 ◽  
Vol 10 (04) ◽  
pp. 265-272
Author(s):  
Letícia Surian Batalini ◽  
Silvana de Oliveira Castro ◽  
Carla Geórgia Rodrigues G. ◽  
Herintha Coeto Neitzke-Abreu ◽  
Manoel Sebastião da Costa L.
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Dna Extraction Methods

scholarly journals Comparison of Three DNA Extraction Methods for Detection of Erysipelothrix Rhusiopathiae in Chicken Blood by Polymerase Chain Reaction

2009 ◽  
Vol 21 (3) ◽  
pp. 354-358 ◽  
Author(s):  
Kazuki Harada ◽  
Mariko Uchiyama ◽  
Teruyuki Hoshi ◽  
Toshio Takahashi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Erysipelothrix Rhusiopathiae ◽  
Chain Reaction ◽  
Detection Techniques ◽  
Chicken Isolate ◽  
Polymerase Chain ◽  
Dna Extraction Methods ◽  
Chicken Blood

A previously reported Erysipelothrix-specific polymerase chain reaction (PCR) was used to detect Erysipelothrix bacteremia in chickens. The sensitivity of PCR using 3 DNA extraction methods (boiling method, commercial gene matrix, and DNA extractor kit) was compared by using a serial 10-fold dilution of a chicken isolate of Erysipelothrix rhusiopathiae strain in chicken blood. Of the techniques used, the DNA extractor kit, followed by PCR, provided the most sensitive method for the detection of the E. rhusiopathiae strain in chicken blood (approximately 100 CFU/0.1 ml of blood). Two E. rhusiopathiae infection experiments were then attempted. In a total of 10 inoculated chickens, bacteremia developed in 9 chickens, consisting of all 5 chickens used in the first trial (ranging from 5.1 × 101 to 2.0 × 103 CFU/0.1 ml of blood) and 4 of the 5 chickens used in the second trial (ranging from 1.0 × 100 to 3.3 × 102 CFU/0.1 ml of blood). In the second trial, the 3 detection techniques were applied to the chickens with bacteremia, and the organism could be detected by using the DNA extractor kit in blood specimens from the 3 chickens exhibiting bacteremia of ≥4.2 × 101 CFU/0.1 ml of blood. This observation suggests that most E. rhusiopathiae–infected chickens develop more critical bacteremia than the detectable level by PCR with the DNA extractor kit, and the PCR detection method can be used as a first-line screening of avian erysipelas.

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