scholarly journals Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

2013 ◽  
Vol 73 (2) ◽  
pp. 425-429 ◽  
Author(s):  
JD Biller-Takahashi ◽  
LS Takahashi ◽  
MV Saita ◽  
RY Gimbo ◽  
EC Urbinati
Keyword(s):  
Respiratory Burst ◽  
Aeromonas Hydrophila ◽  
Innate Immune System ◽  
Innate Immune ◽  
Burst Activity ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Neotropical Fish ◽  
Blood Leukocytes ◽  
Experimental Conditions

The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.

scholarly journals Innate immune responses of whiteleg shrimp (Penaeus vannamei) experimentally infected with acute hepatopancreas necrosis disease-causing Vibbrio parahaemolyticus

2019 ◽  
Vol 18 (01) ◽  
pp. 80-88
Author(s):  
Tuan V. Vo
Keyword(s):  
Immune Responses ◽  
Respiratory Burst ◽  
Innate Immune ◽  
Burst Activity ◽  
Penaeus Vannamei ◽  
Innate Immune Responses ◽  
Respiratory Burst Activity ◽  
Phenoloxidase Activity ◽  
Whiteleg Shrimp ◽  
Total Haemocyte Count

The innate immune responses of the whiteleg shrimps (Penaeus vannamei) experimentally challenged with V. parahaemolyticus by immersion were investigated for a period of 120 h. The results showed that the lethal dose 50% (LD50) of shrimps (2 - 3 g) challenged with V. parahaemolyticus was 4.7 × 106 CFU/mL. No significant differences in immune parameters were observed between the control and challenged group right after challenge (0 hpi). However, the total haemocyte count, phenoloxidase activity and respiratory burst activity were decreased in the challenged shrimps after 24 and 48 hpi and significantly different from those in the control shrimps (P < 0,05). At 72, 96 and 120 hpi, there were no significant differences in the total haemocyte count, phenoloxidase activity and respiratory burst activity between two treatments. The observations of this study showed that the innate immune responses of the whiteleg shrimp were decreased due to the infection by V. parahaemolyticus.

scholarly journals Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Luis A. Videla ◽  
Pamela Cornejo ◽  
Pamela Romanque ◽  
Catherine Santibáñez ◽  
Iván Castillo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Kupffer Cell ◽  
Respiratory Burst ◽  
Oxidase Inhibitor ◽  
Burst Activity ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Nadph Oxidase Inhibitor ◽  
Nrf2 Activation

L-3,3′,5-triiodothyronine (T3) administration upregulates nuclear factor-E2-related factor 2 (Nrf2) in rat liver, which is redox-sensitive transcription factor mediating cytoprotection. In this work, we studied the role of Kupffer cell respiratory burst activity, a process related to reactive oxygen species generation and liver homeostasis, in Nrf2 activation using the macrophage inactivator gadolinium chloride (GdCl3; 10 mg/kg i.v. 72 h before T3[0.1 mg/kg i.p.]) or NADPH oxidase inhibitor apocynin (1.5 mmol/L added to the drinking water for 7 days before T3), and determinations were performed 2 h after T3. T3increased nuclear/cytosolic Nrf2 content ratio and levels of heme oxygenase 1 (HO-1), catalytic subunit of glutamate cysteine ligase, and thioredoxin (Western blot) over control values, proteins whose gene transcription is induced by Nrf2. These changes were suppressed by GdCl3treatment prior to T3, an agent-eliciting Kupffer-cell depletion, inhibition of colloidal carbon phagocytosis, and the associated respiratory burst activity, with enhancement in nuclear inhibitor of Nrf2 kelch-like ECH-associated protein 1 (Keap1)/Nrf2 content ratios suggesting Nrf2 degradation. Under these conditions, T3-induced tumor necrosis factor-α(TNF-α) response was eliminated by previous GdCl3administration. Similar to GdCl3, apocynin given before T3significantly reduced liver Nrf2 activation and HO-1 expression, a NADPH oxidase inhibitor eliciting abolishment of colloidal carbon-induced respiratory burst activity without altering carbon phagocytosis. It is concluded that Kupffer cell functioning is essential for upregulation of liver Nrf2-signaling pathway by T3. This contention is supported by suppression of the respiratory burst activity of Kupffer cells and the associated reactive oxygen species production by GdCl3or apocynin given prior to T3, thus hindering Nrf2 activation.

scholarly journals The respiratory explosion activity of neutrophils in blood from patients with Graves' disease manifestation

2017 ◽  
Vol 63 (1) ◽  
pp. 4-8
Author(s):  
Sergey A. Dogadin ◽  
Margarita A. Dudina ◽  
Andrey A. Savchenko ◽  
Vladimir A. Mankovskiy ◽  
Ivan I. Gvozdev
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Area Under The Curve ◽  
Reactive Oxygen ◽  
Maximum Level ◽  
Burst Activity ◽  
Graves Disease ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Disease Manifestation

  Objective. To study respiratory burst activity in neutrophilic granulocytes in the onset of Graves’ disease (GD). Material and methods. Twenty-six females aged 18—55 years (mean age, 40.7±13.2 years), with the diagnosis of Graves’ disease verified for the first time, were enrolled in this study. Spontaneous and zymosan-induced chemiluminescence (CL) was assessed using a CL3606 36-channel analyzer for 90 min. The following parameters of the chemiluminescence curve were determined: time to attain the maximum (Tmax), the maximum intensity (Imax), and the area under the curve (S). Results. Patients with GD and lucigenin-dependent CL of neutrophils exhibited decreased zymosan-induced CL and the increased Imax of luminol-dependent spontaneous and zymosan-induced CL of neutrophils. Association between the anti-TPO level in blood serum with Tmax (r=–0,70; р=0.036) and Imax of luminol-dependent induced CL (r=–0.72; р=0.030) was found. The maximum level of synthesis of secondary reactive oxygen species (ROS) was found to be elevated in patients with GP both at relative rest and upon antigen-induced respiratory burst. Conclusion. In patients with GD, respiratory burst activity in neutrophils increases due to synthesis of secondary reactive oxygen species. Upon the onset of GD, already at the stage of subclinical thyrotoxicosis, the association between the indices of respiratory burst in neutrophils and thyroid hormones is lost but there emerges a relationship between the CL response and anti-TPO concentration.

scholarly journals Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation

2018 ◽  
Vol 12 (10) ◽  
pp. 894-903
Author(s):  
Marina Žekić-Stošić ◽  
Zdenko Kanački ◽  
Dragica Stojanović ◽  
Dejan Bugarski ◽  
Miodrag Lazarević ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Respiratory Burst ◽  
Transition Period ◽  
Hematological Parameters ◽  
Optimal Level ◽  
Burst Activity ◽  
Early Lactation ◽  
Health Production ◽  
Respiratory Burst Activity ◽  
Entire Study

Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 ± 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 ± 5.22% at the beginning of the study to 89.54 ± 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with β-hydroxybutyrat and non-esterified fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.

Ketamine inhibits polymorphonuclear leucocyte CD11b expression and respiratory burst activity in endotoxemic rats

2007 ◽  
Vol 56 (4) ◽  
pp. 149-153 ◽  
Author(s):  
Z. Q. Zhou ◽  
Y. Q. Yu ◽  
S. W. Feng ◽  
M. Yu ◽  
H. J. Liu ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Polymorphonuclear Leucocyte ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Cd11b Expression
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