Interspecific Hybridization as a Source of Novel Genetic Markers for the Sterile Insect Technique in Bactrocera tryoni (Diptera: Tephritidae)

2010 ◽  
Vol 103 (4) ◽  
pp. 1071-1079 ◽  
Author(s):  
D. C. A. Shearman ◽  
M. Frommer ◽  
J. L. Morrow ◽  
K. A. Raphael ◽  
A. S. Gilchrist
Keyword(s):  
Genetic Markers ◽  
Interspecific Hybridization ◽  
Sterile Insect Technique ◽  
Bactrocera Tryoni

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

Effect of natural predators on Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) control by sterile insect technique (SIT)

2019 ◽  
Vol 75 (12) ◽  
pp. 3356-3362 ◽  
Author(s):  
Darshana N Rathnayake ◽  
Elizabeth C Lowe ◽  
Polychronis Rempoulakis ◽  
Marie E Herberstein
Keyword(s):  
Sterile Insect Technique ◽  
Fruit Fly ◽  
Bactrocera Tryoni

Short- and long-range dispersal of the Queensland fruit fly, Bactrocera tryoni and its relevance to invasive potential, sterile insect technique and surveillance trapping

2008 ◽  
Vol 48 (9) ◽  
pp. 1237 ◽  
Author(s):  
A. Meats ◽  
J. E. Edgerton
Keyword(s):  
Sterile Insect Technique ◽  
Single Point ◽  
Cauchy Distribution ◽  
Fruit Fly ◽  
Bactrocera Tryoni ◽  
Scale Invariant ◽  
Long Distance ◽  
Negative Power ◽  
Catch Rates ◽  
Cauchy Model

Dispersal of immature and sexually mature Queensland fruit fly, Bactrocera tryoni (Froggatt) from releases made at a single point was assessed from recapture rates obtained by using arrays of traps. The recapture data (pertaining to distances up to 480 m) fitted both logarithmic and Cauchy models although the fits for the releases of immature flies were inferior because of high variability in catches at certain distances. When combined with data previously published for longer distances, a Cauchy model fitted data for releases of immature flies well and indicated that the median distance dispersed after emerging from the puparium was ~120 m and that 90% of flies would displace less than 800 m despite the fact that a consistent trend in declining catch rates can be obtained up to at least 85 km. This is consistent with the tail of the Cauchy distribution having a slope congruent with a negative power curve and thus being scale invariant for longer distances. The distribution of recaptured flies that were released as adults also fitted a Cauchy model with a tail of the same slope, suggesting that the spatial distribution of long-distance dispersers is not only scale invariant but also age invariant. This has significance to the ability of surveillance trapping arrays to detect infestations and also to methods of distributing insects for the sterile insect technique. Whereas the spread of invading propagules in the first generation is likely to be limited by a decline to non-viable density within 1 km or less of the incursion point, the influence of larger infestations on nearby uninfested regions would be limited by the longevity of the dispersers.

scholarly journals Desiccation resistance of wild and mass-reared Bactrocera tryoni (Diptera: Tephritidae)

2013 ◽  
Vol 103 (6) ◽  
pp. 690-699 ◽  
Author(s):  
C.W. Weldon ◽  
S. Yap ◽  
P.W. Taylor
Keyword(s):  
Water Stress ◽  
Sterile Insect Technique ◽  
Reproductive Development ◽  
Desiccation Resistance ◽  
Sharp Decline ◽  
Bactrocera Tryoni ◽  
Dehydration Tolerance ◽  
Dry Conditions ◽  
Access To Food ◽  
Adult Mass

AbstractIn pest management programmes that incorporate the sterile insect technique (SIT), the ability of mass-reared insects to tolerate dry conditions may influence their survival after release in the field. In the present study, desiccation resistance of adult mass-reared Queensland fruit flies, Bactrocera tryoni (Frogatt) (Diptera: Tephritidae), that are routinely released in SIT programmes was compared with that of wild flies at 1, 10 and 20 days after adult eclosion. Under dry conditions without access to food or water, longevity of mass-reared B. tryoni was significantly less than that of their wild counterparts. Desiccation resistance of mass-reared flies declined monotonically with age, but this was not the case for wild flies. The sharp decline in desiccation resistance of mass-reared flies as they aged was likely explained by decreased dehydration tolerance. As in an earlier study, desiccation resistance of females was significantly lower than that of males but this was particularly pronounced in mass-reared females. Female susceptibility to dry conditions corresponded with declining dehydration tolerance with age and associated patterns of reproductive development, which suggests that water content of their oocyte load is not available for survival during periods of water stress.

scholarly journals Semiochemical mediated enhancement of males to complement sterile insect technique in management of the tephritid pest Bactrocera tryoni (Froggatt)

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohammed Abul Monjur Khan ◽  
Nicholas C. Manoukis ◽  
Terry Osborne ◽  
Idris M. Barchia ◽  
Geoff M. Gurr ◽  
...  
Keyword(s):  
Sterile Insect Technique ◽  
Bactrocera Tryoni

Visibility and Persistence of Fluorescent Dyes, and Impacts on Emergence, Quality, and Survival of Sterile Queensland Fruit Fly Bactrocera tryoni (Diptera: Tephritidae)

2020 ◽  
Vol 113 (6) ◽  
pp. 2800-2807
Author(s):  
Humayra Akter ◽  
Phillip W Taylor ◽  
Peter Crisp
Keyword(s):  
Sterile Insect Technique ◽  
Fluorescent Dyes ◽  
Survival Rates ◽  
Adult Emergence ◽  
Fruit Fly ◽  
Bactrocera Tryoni ◽  
Similar Dose ◽  
Monitoring Traps ◽  
Dose Levels ◽  
Dose Dependent

Abstract Tephritid flies released in sterile insect technique pest management programs are usually marked with fluorescent dyes so that they can be distinguished from wild flies in monitoring traps. Dyes can have adverse effects on emergence, quality, and survival, which can impact sterile insect technique (SIT) success, and so it is important to identify dyes and doses that maximize marking efficacy while minimizing deleterious effects on fly quality. This study examines the effects of five fluorescent dye products, Fluoro Pink, Fluoro Orange, Stella Green, Arc Chrome, and Astral Pink applied at four dose levels (1, 2, 3, and 4 g/liter) on Queensland fruit fly. All dye products caused a similar dose-dependent reduction in percentage of adult emergence. Incidence of morphological deformity of emerged adults increased with dose, and this trend was similar for all dye products. No effects of dye product or dose were found on survival rates over the first 35 d of adulthood, although females tended to have higher survival than males. Visibility varied with dose and dye product; 1 g/liter dye was less visible than 2, 3, or 4 g/liter, and Stella green had lower visibility than other dyes. All of the tested dyes except for Stella green were similar in all assessed metrics of fly performance and are recommended for use in SIT programs.

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