Genetic Structure and Gene Flow Among Brazilian Populations of Heliothis virescens (Lepidoptera: Noctuidae)

2012 ◽  
Vol 105 (6) ◽  
pp. 2136-2146 ◽  
Author(s):  
Felipe A. Domingues ◽  
Karina L. Silva-Brandão ◽  
Aluana G. Abreu ◽  
Omaththage P. Perera ◽  
Carlos A. Blanco ◽  
...  

2017 ◽  
Vol 107 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Danilo A. dos Santos Pereira ◽  
Paulo C. Ceresini ◽  
Vanina L. Castroagudín ◽  
Lina M. Ramos-Molina ◽  
Edisson Chavarro-Mesa ◽  
...  

The fungus Rhizoctonia oryzae-sativae is an important pathogen that causes the aggregated sheath spot disease on rice. In this study, we investigated the genetic structure of rice-adapted populations of R. oryzae-sativae sampled from traditional rice-cropping areas from the Paraíba Valley, São Paulo, Brazil, and from Meta, in the Colombian Llanos, in South America. We used five microsatellite loci to measure population differentiation and infer the pathogen’s reproductive system. Gene flow was detected among the three populations of R. oryzae-sativae from lowland rice in Brazil and Colombia. In contrast, a lack of gene flow was observed between the lowland and the upland rice populations of the pathogen. Evidence of sexual reproduction including low clonality, Hardy-Weinberg equilibrium within loci and gametic equilibrium between loci, indicated the predominance of a mixed reproductive system in all populations. In addition, we assessed the adaptive potential of the Brazilian populations of R. oryzae-sativae to emerge as a pathogen to Urochloa spp. (signalgrass) based on greenhouse aggressiveness assays. The Brazilian populations of R. oryzae-sativae were probably only incipiently adapted as a pathogen to Urochloa spp. Comparison between RST and QST showed the predominance of diversifying selection in the divergence between the two populations of R. oryzae-sativae from Brazil.



2015 ◽  
Vol 8 (5) ◽  
pp. 476-485 ◽  
Author(s):  
Karina Lucas Silva‐Brandão ◽  
Oscar Arnaldo Batista Neto e Silva ◽  
Marcelo Mendes Brandão ◽  
Celso Omoto ◽  
Felix A. H. Sperling


2019 ◽  
Vol 9 (23) ◽  
pp. 13426-13437
Author(s):  
Mei‐Mei Li ◽  
Bo‐Liao Li ◽  
Shi‐Xiong Jiang ◽  
Yu‐Wan Zhao ◽  
Xiang‐Li Xu ◽  
...  


2017 ◽  
Vol 108 (5) ◽  
pp. 636-644 ◽  
Author(s):  
W.-C. Zhu ◽  
J.-T. Sun ◽  
J. Dai ◽  
J.-R. Huang ◽  
L. Chen ◽  
...  

AbstractAthetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a new outbreak pest in China. Consequently, it is unclear whether the emergence and spread of the outbreak of this pest are triggered by rapid in situ population size increases in each outbreak area, or by immigrants from a potential source area in China. In order to explore the outbreak process of this pest through a population genetics approach, we developed ten novel polymorphic expressed sequence tags (EST)-derived microsatellites. These new microsatellites had moderately high levels of polymorphism in the tested population. The number of alleles per locus ranged from 3 to 19, with an average of 8.6, and the expected heterozygosity ranged from 0.269 to 0.783. A preliminary population genetic analysis using these new microsatellites revealed a lack of population genetic structure in natural populations of A. lepigone. The estimates of recent migration rate revealed strong gene flow among populations. In conclusion, our study developed the first set of EST-microsatellite markers and shed a new light on the population genetic structure of this pest in China.



1993 ◽  
Vol 86 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Amy K. Korman ◽  
James Mallet ◽  
John L. Goodenough ◽  
Jerry B. Graves ◽  
Jane L. Hayes ◽  
...  


PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0197378 ◽  
Author(s):  
Karina Lucas Silva-Brandão ◽  
Aline Peruchi ◽  
Noemy Seraphim ◽  
Natália Faraj Murad ◽  
Renato Assis Carvalho ◽  
...  


Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 643-655 ◽  
Author(s):  
Kenneth G Ross ◽  
Michael J B Krieger ◽  
D DeWayne Shoemaker ◽  
Edward L Vargo ◽  
Laurent Keller

We describe genetic structure at various scales in native populations of the fire ant Solenopsis invicta using two classes of nuclear markers, allozymes and microsatellites, and markers of the mitochondrial genome. Strong structure was found at the nest level in both the monogyne (single queen) and polygyne (multiple queen) social forms using allozymes. Weak but significant microgeographic structure was detected above the nest level in polygyne populations but not in monogyne populations using both classes of nuclear markers. Pronounced mitochondrial DNA (mtDNA) differentiation was evident also at this level in the polygyne form only. These microgeographic patterns are expected because polygyny in ants is associated with restricted local gene flow due mainly to limited vagility of queens. Weak but significant nuclear differentiation was detected between sympatric social forms, and strong mtDNA differentiation also was found at this level. Thus, queens of each form seem unable to establish themselves in nests of the alternate type, and some degree of assortative mating by form may exist as well. Strong differentiation was found between the two study regions usinga all three sets of markers. Phylogeographic analyses of the mtDNA suggest that recent limitations on gene flow rather than longstanding barriers to dispersal are responsible for this large-scale structure.



Genetics ◽  
1997 ◽  
Vol 147 (4) ◽  
pp. 1843-1854 ◽  
Author(s):  
Nancy N FitzSimmons ◽  
Craig Moritz ◽  
Colin J Limpus ◽  
Lisa Pope ◽  
Robert Prince

Abstract The genetic structure of green turtle (Chelonia mydas) rookeries located around the Australian coast was assessed by (1) comparing the structure found within and among geographic regions, (2) comparing microsatellite loci vs. restriction fragment length polymorphism analyses of anonymous single copy nuclear DNA (ascnDNA) loci, and (3) comparing the structure found at nuclear DNA markers to that of previously analyzed mitochondrial (mtDNA) control region sequences. Significant genetic structure was observed over all regions at both sets of nuclear markers, though the microsatellite data provided greater resolution in identifying significant genetic differences in pairwise tests between regions. Inferences about population structure and migration rates from the microsatellite data varied depending on whether statistics were based on the stepwise mutation or infinite allele model, with the latter being more congruent with geography. Estimated rates of gene flow were generally higher than expected for nuclear DNA (nDNA) in comparison to mtDNA, and this difference was most pronounced in comparisons between the northern and southern Great Barrier Reef (GBR). The genetic data combined with results from physical tagging studies indicate that the lack of nuclear gene divergence through the GBR is likely due to the migration of sGBR turtles through the courtship area of the nGBR population, rather than male-biased dispersal. This example highlights the value of combining comparative studies of molecular variation with ecological data to infer population processes.







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