scholarly journals Microsatellite‐based analysis of genetic structure and gene flow of Mythimna separata (Walker) (Lepidoptera: Noctuidae) in China

2019 ◽  
Vol 9 (23) ◽  
pp. 13426-13437
Author(s):  
Mei‐Mei Li ◽  
Bo‐Liao Li ◽  
Shi‐Xiong Jiang ◽  
Yu‐Wan Zhao ◽  
Xiang‐Li Xu ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Mythimna Separata ◽  
Lepidoptera Noctuidae

Genetic Structure and Gene Flow Among Brazilian Populations of Heliothis virescens (Lepidoptera: Noctuidae)

2012 ◽  
Vol 105 (6) ◽  
pp. 2136-2146 ◽  
Author(s):  
Felipe A. Domingues ◽  
Karina L. Silva-Brandão ◽  
Aluana G. Abreu ◽  
Omaththage P. Perera ◽  
Carlos A. Blanco ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Heliothis Virescens ◽  
Lepidoptera Noctuidae ◽  
Brazilian Populations

New microsatellites revealed strong gene flow among populations of a new outbreak pest, Athetis lepigone (Möschler)

2017 ◽  
Vol 108 (5) ◽  
pp. 636-644 ◽  
Author(s):  
W.-C. Zhu ◽  
J.-T. Sun ◽  
J. Dai ◽  
J.-R. Huang ◽  
L. Chen ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Natural Populations ◽  
Source Area ◽  
Population Genetic Analysis ◽  
Outbreak Area ◽  
Lepidoptera Noctuidae

AbstractAthetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a new outbreak pest in China. Consequently, it is unclear whether the emergence and spread of the outbreak of this pest are triggered by rapid in situ population size increases in each outbreak area, or by immigrants from a potential source area in China. In order to explore the outbreak process of this pest through a population genetics approach, we developed ten novel polymorphic expressed sequence tags (EST)-derived microsatellites. These new microsatellites had moderately high levels of polymorphism in the tested population. The number of alleles per locus ranged from 3 to 19, with an average of 8.6, and the expected heterozygosity ranged from 0.269 to 0.783. A preliminary population genetic analysis using these new microsatellites revealed a lack of population genetic structure in natural populations of A. lepigone. The estimates of recent migration rate revealed strong gene flow among populations. In conclusion, our study developed the first set of EST-microsatellite markers and shed a new light on the population genetic structure of this pest in China.

Transcriptional identification of differentially expressed genes during the prepupal–pupal transition in the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae)

2021 ◽  
pp. 1-14
Author(s):  
Peirong Li ◽  
Xinru Li ◽  
Wei Wang ◽  
Xiaoling Tan ◽  
Xiaoqi Wang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Metabolic Pathways ◽  
Developmental Stages ◽  
Kegg Pathway ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Mythimna Separata ◽  
Kegg Pathway Analysis ◽  
Oriental Armyworm ◽  
Lepidoptera Noctuidae

Abstract The oriental armyworm, Mythimna separata (Walker) is a serious pest of agriculture that does particular damage to Gramineae crops in Asia, Europe, and Oceania. Metamorphosis is a key developmental stage in insects, although the genes underlying the metamorphic transition in M. separata remain largely unknown. Here, we sequenced the transcriptomes of five stages; mature larvae (ML), wandering (W), and pupation (1, 5, and 10 days after pupation, designated P1, P5, and P10) to identify transition-associated genes. Four libraries were generated, with 22,884, 23,534, 26,643, and 33,238 differentially expressed genes (DEGs) for the ML-vs-W, W-vs-P1, P1-vs-P5, and P5-vs-P10, respectively. Gene ontology enrichment analysis of DEGs showed that genes regulating the biosynthesis of the membrane and integral components of the membrane, which includes the cuticular protein (CP), 20-hydroxyecdysone (20E), and juvenile hormone (JH) biosynthesis, were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that DEGs were enriched in the metabolic pathways. Of these DEGs, thirty CP, seventeen 20E, and seven JH genes were differentially expressed across the developmental stages. For transcriptome validation, ten CP, 20E, and JH-related genes were selected and verified by real-time PCR quantitative. Collectively, our results provided a basis for further studies of the molecular mechanism of metamorphosis in M. separata.

scholarly journals Hierarchical Analysis of Genetic Structure in Native Fire Ant Populations: Results From Three Classes of Molecular Markers

Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 643-655 ◽  
Author(s):  
Kenneth G Ross ◽  
Michael J B Krieger ◽  
D DeWayne Shoemaker ◽  
Edward L Vargo ◽  
Laurent Keller
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Solenopsis Invicta ◽  
Large Scale ◽  
Fire Ant ◽  
Nuclear Markers ◽  
Social Forms ◽  
Native Populations ◽  
Nest Level ◽  
Nuclear Differentiation

We describe genetic structure at various scales in native populations of the fire ant Solenopsis invicta using two classes of nuclear markers, allozymes and microsatellites, and markers of the mitochondrial genome. Strong structure was found at the nest level in both the monogyne (single queen) and polygyne (multiple queen) social forms using allozymes. Weak but significant microgeographic structure was detected above the nest level in polygyne populations but not in monogyne populations using both classes of nuclear markers. Pronounced mitochondrial DNA (mtDNA) differentiation was evident also at this level in the polygyne form only. These microgeographic patterns are expected because polygyny in ants is associated with restricted local gene flow due mainly to limited vagility of queens. Weak but significant nuclear differentiation was detected between sympatric social forms, and strong mtDNA differentiation also was found at this level. Thus, queens of each form seem unable to establish themselves in nests of the alternate type, and some degree of assortative mating by form may exist as well. Strong differentiation was found between the two study regions usinga all three sets of markers. Phylogeographic analyses of the mtDNA suggest that recent limitations on gene flow rather than longstanding barriers to dispersal are responsible for this large-scale structure.

Biogenetic cantharidin is a promising leading compound to manage insecticide resistance of Mythimna separata (Lepidoptera: Noctuidae)

2021 ◽  
pp. 104769
Author(s):  
Yifan Li ◽  
Hong Sun ◽  
Hassan Yasoob ◽  
Zhen Tian ◽  
Yue Li ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Mythimna Separata ◽  
Lepidoptera Noctuidae

scholarly journals Geographic Structure of Mitochondrial and Nuclear Gene Polymorphisms in Australian Green Turtle Populations and Male-Biased Gene Flow

Genetics ◽  
1997 ◽  
Vol 147 (4) ◽  
pp. 1843-1854 ◽  
Author(s):  
Nancy N FitzSimmons ◽  
Craig Moritz ◽  
Colin J Limpus ◽  
Lisa Pope ◽  
Robert Prince
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Green Turtle ◽  
Nuclear Dna ◽  
Nuclear Gene ◽  
Chelonia Mydas ◽  
Single Copy ◽  
Microsatellite Data ◽  
Ecological Data ◽  
Infinite Allele Model

Abstract The genetic structure of green turtle (Chelonia mydas) rookeries located around the Australian coast was assessed by (1) comparing the structure found within and among geographic regions, (2) comparing microsatellite loci vs. restriction fragment length polymorphism analyses of anonymous single copy nuclear DNA (ascnDNA) loci, and (3) comparing the structure found at nuclear DNA markers to that of previously analyzed mitochondrial (mtDNA) control region sequences. Significant genetic structure was observed over all regions at both sets of nuclear markers, though the microsatellite data provided greater resolution in identifying significant genetic differences in pairwise tests between regions. Inferences about population structure and migration rates from the microsatellite data varied depending on whether statistics were based on the stepwise mutation or infinite allele model, with the latter being more congruent with geography. Estimated rates of gene flow were generally higher than expected for nuclear DNA (nDNA) in comparison to mtDNA, and this difference was most pronounced in comparisons between the northern and southern Great Barrier Reef (GBR). The genetic data combined with results from physical tagging studies indicate that the lack of nuclear gene divergence through the GBR is likely due to the migration of sGBR turtles through the courtship area of the nGBR population, rather than male-biased dispersal. This example highlights the value of combining comparative studies of molecular variation with ecological data to infer population processes.

Population genetic structure and gene flow in the Japanese sea cucumber Apostichopus japonicus across Toyama Bay, Japan

2012 ◽  
Vol 78 (4) ◽  
pp. 775-783 ◽  
Author(s):  
Taha Soliman ◽  
Manami Kanno ◽  
Akihiro Kijima ◽  
Yuji Yamazaki
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Sea Cucumber ◽  
Apostichopus Japonicus ◽  
Toyama Bay ◽  
Japanese Sea Cucumber

scholarly journals Population Genetic Structure of the Cotton Bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in India as Inferred from EPIC-PCR DNA Markers

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53448 ◽  
Author(s):  
Gajanan Tryambak Behere ◽  
Wee Tek Tay ◽  
Derek Alan Russell ◽  
Keshav Raj Kranthi ◽  
Philip Batterham
Keyword(s):  
Genetic Structure ◽  
Helicoverpa Armigera ◽  
Population Genetic Structure ◽  
Dna Markers ◽  
Population Genetic ◽  
Cotton Bollworm ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae ◽  
Helicoverpa Armigera Hubner
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